KinATLAS
TranscriptoNET
PhosphoNET
OncoNET
KinaseNET
DrugKiNET
DrugProNET
KiNET-AM
Kinetica Online
Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
Home
|
Kinexus
|
Contact
|
Credits
Protein Info
Short Name:
PIK3R1
Full Name:
Phosphatidylinositol 3-kinase regulatory subunit alpha
Alias:
GRB1; P85; P85A; P85-ALPHA; Phosphatidylinositol 3-kinase regulatory alpha subunit; Phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3K; PI3K p85-alpha; PI3-kinase p85-alpha; PtdIns-3-kinase p85-alpha
Type:
Regulator, inositol phosphate metabolism
Mass (Da):
83598
Number AA:
724
UniProt ID:
P27986
International Prot ID:
IPI00021448
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005943
Uniprot
OncoNet
Molecular Function:
GO:0043125
GO:0043559
GO:0005158
PhosphoSite+
KinaseNET
Biological Process:
GO:0060396
GO:0008286
GO:0048009
Phosida
TranscriptoNet
STRING
Kinexus Products
Phosphatidylinositol 3-kinase regulatory subunit alpha Y467 phosphosite-specific antibody AB-PN526#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN526#Phosphatidylinositol 3-kinase regulatory subunit alpha Y580 phosphosite-specific antibody AB-PN527#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN527#Phosphatidylinositol 3-kinase regulatory subunit alpha (R577-W583, human) pY580 phosphopeptide - Powder PE-04AEX85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AEX85#Phosphatidylinositol 3-kinase regulatory subunit alpha (D464-Y470, human) pY467 phosphopeptide - Powder PE-04AHI99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AHI99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y8
M
S
A
E
G
Y
Q
Y
R
A
L
Y
D
Y
K
Site 2
Y12
G
Y
Q
Y
R
A
L
Y
D
Y
K
K
E
R
E
Site 3
Y14
Q
Y
R
A
L
Y
D
Y
K
K
E
R
E
E
D
Site 4
Y59
E
I
G
W
L
N
G
Y
N
E
T
T
G
E
R
Site 5
T72
E
R
G
D
F
P
G
T
Y
V
E
Y
I
G
R
Site 6
Y73
R
G
D
F
P
G
T
Y
V
E
Y
I
G
R
K
Site 7
Y76
F
P
G
T
Y
V
E
Y
I
G
R
K
K
I
S
Site 8
S83
Y
I
G
R
K
K
I
S
P
P
T
P
K
P
R
Site 9
T86
R
K
K
I
S
P
P
T
P
K
P
R
P
P
R
Site 10
S101
P
L
P
V
A
P
G
S
S
K
T
E
A
D
V
Site 11
S147
E
K
K
G
L
E
C
S
T
L
Y
R
T
Q
S
Site 12
T148
K
K
G
L
E
C
S
T
L
Y
R
T
Q
S
S
Site 13
Y150
G
L
E
C
S
T
L
Y
R
T
Q
S
S
S
N
Site 14
T152
E
C
S
T
L
Y
R
T
Q
S
S
S
N
L
A
Site 15
S154
S
T
L
Y
R
T
Q
S
S
S
N
L
A
E
L
Site 16
S156
L
Y
R
T
Q
S
S
S
N
L
A
E
L
R
Q
Site 17
T169
R
Q
L
L
D
C
D
T
P
S
V
D
L
E
M
Site 18
Y189
L
A
D
A
F
K
R
Y
L
L
D
L
P
N
P
Site 19
Y203
P
V
I
P
A
A
V
Y
S
E
M
I
S
L
A
Site 20
S216
L
A
P
E
V
Q
S
S
E
E
Y
I
Q
L
L
Site 21
S229
L
L
K
K
L
I
R
S
P
S
I
P
H
Q
Y
Site 22
S231
K
K
L
I
R
S
P
S
I
P
H
Q
Y
W
L
Site 23
Y236
S
P
S
I
P
H
Q
Y
W
L
T
L
Q
Y
L
Site 24
T239
I
P
H
Q
Y
W
L
T
L
Q
Y
L
L
K
H
Site 25
S251
L
K
H
F
F
K
L
S
Q
T
S
S
K
N
L
Site 26
S255
F
K
L
S
Q
T
S
S
K
N
L
L
N
A
R
Site 27
S265
L
L
N
A
R
V
L
S
E
I
F
S
P
M
L
Site 28
S276
S
P
M
L
F
R
F
S
A
A
S
S
D
N
T
Site 29
S279
L
F
R
F
S
A
A
S
S
D
N
T
E
N
L
Site 30
S280
F
R
F
S
A
A
S
S
D
N
T
E
N
L
I
Site 31
T283
S
A
A
S
S
D
N
T
E
N
L
I
K
V
I
Site 32
S295
K
V
I
E
I
L
I
S
T
E
W
N
E
R
Q
Site 33
T316
P
K
P
P
K
P
T
T
V
A
N
N
G
M
N
Site 34
Y334
S
L
Q
D
A
E
W
Y
W
G
D
I
S
R
E
Site 35
T350
V
N
E
K
L
R
D
T
A
D
G
T
F
L
V
Site 36
T354
L
R
D
T
A
D
G
T
F
L
V
R
D
A
S
Site 37
S361
T
F
L
V
R
D
A
S
T
K
M
H
G
D
Y
Site 38
Y368
S
T
K
M
H
G
D
Y
T
L
T
L
R
K
G
Site 39
T369
T
K
M
H
G
D
Y
T
L
T
L
R
K
G
G
Site 40
T371
M
H
G
D
Y
T
L
T
L
R
K
G
G
N
N
Site 41
Y390
I
F
H
R
D
G
K
Y
G
F
S
D
P
L
T
Site 42
S393
R
D
G
K
Y
G
F
S
D
P
L
T
F
S
S
Site 43
Y408
V
V
E
L
I
N
H
Y
R
N
E
S
L
A
Q
Site 44
S412
I
N
H
Y
R
N
E
S
L
A
Q
Y
N
P
K
Site 45
Y416
R
N
E
S
L
A
Q
Y
N
P
K
L
D
V
K
Site 46
Y426
K
L
D
V
K
L
L
Y
P
V
S
K
Y
Q
Q
Site 47
S429
V
K
L
L
Y
P
V
S
K
Y
Q
Q
D
Q
V
Site 48
Y431
L
L
Y
P
V
S
K
Y
Q
Q
D
Q
V
V
K
Site 49
Y452
V
G
K
K
L
H
E
Y
N
T
Q
F
Q
E
K
Site 50
T454
K
K
L
H
E
Y
N
T
Q
F
Q
E
K
S
R
Site 51
S460
N
T
Q
F
Q
E
K
S
R
E
Y
D
R
L
Y
Site 52
Y463
F
Q
E
K
S
R
E
Y
D
R
L
Y
E
E
Y
Site 53
Y467
S
R
E
Y
D
R
L
Y
E
E
Y
T
R
T
S
Phosphatidylinositol 3-kinase regulatory subunit alpha Y467 phosphosite-specific antibody AB-PN526#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN526#Phosphatidylinositol 3-kinase regulatory subunit alpha (D464-Y470, human) pY467 phosphopeptide - Powder PE-04AHI99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AHI99
Site 54
Y470
Y
D
R
L
Y
E
E
Y
T
R
T
S
Q
E
I
Site 55
T471
D
R
L
Y
E
E
Y
T
R
T
S
Q
E
I
Q
Site 56
T473
L
Y
E
E
Y
T
R
T
S
Q
E
I
Q
M
K
Site 57
S474
Y
E
E
Y
T
R
T
S
Q
E
I
Q
M
K
R
Site 58
T490
A
I
E
A
F
N
E
T
I
K
I
F
E
E
Q
Site 59
T500
I
F
E
E
Q
C
Q
T
Q
E
R
Y
S
K
E
Site 60
Y504
Q
C
Q
T
Q
E
R
Y
S
K
E
Y
I
E
K
Site 61
Y508
Q
E
R
Y
S
K
E
Y
I
E
K
F
K
R
E
Site 62
Y528
I
Q
R
I
M
H
N
Y
D
K
L
K
S
R
I
Site 63
S533
H
N
Y
D
K
L
K
S
R
I
S
E
I
I
D
Site 64
S536
D
K
L
K
S
R
I
S
E
I
I
D
S
R
R
Site 65
S541
R
I
S
E
I
I
D
S
R
R
R
L
E
E
D
Site 66
Y556
L
K
K
Q
A
A
E
Y
R
E
I
D
K
R
M
Site 67
S565
E
I
D
K
R
M
N
S
I
K
P
D
L
I
Q
Site 68
T576
D
L
I
Q
L
R
K
T
R
D
Q
Y
L
M
W
Site 69
Y580
L
R
K
T
R
D
Q
Y
L
M
W
L
T
Q
K
Phosphatidylinositol 3-kinase regulatory subunit alpha Y580 phosphosite-specific antibody AB-PN527#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PN527#Phosphatidylinositol 3-kinase regulatory subunit alpha (R577-W583, human) pY580 phosphopeptide - Powder PE-04AEX85#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AEX85
Site 70
T603
E
W
L
G
N
E
N
T
E
D
Q
Y
S
L
V
Site 71
Y607
N
E
N
T
E
D
Q
Y
S
L
V
E
D
D
E
Site 72
S608
E
N
T
E
D
Q
Y
S
L
V
E
D
D
E
D
Site 73
T623
L
P
H
H
D
E
K
T
W
N
V
G
S
S
N
Site 74
S628
E
K
T
W
N
V
G
S
S
N
R
N
K
A
E
Site 75
S629
K
T
W
N
V
G
S
S
N
R
N
K
A
E
N
Site 76
T645
L
R
G
K
R
D
G
T
F
L
V
R
E
S
S
Site 77
S651
G
T
F
L
V
R
E
S
S
K
Q
G
C
Y
A
Site 78
S652
T
F
L
V
R
E
S
S
K
Q
G
C
Y
A
C
Site 79
Y657
E
S
S
K
Q
G
C
Y
A
C
S
V
V
V
D
Site 80
Y679
I
N
K
T
A
T
G
Y
G
F
A
E
P
Y
N
Site 81
Y685
G
Y
G
F
A
E
P
Y
N
L
Y
S
S
L
K
Site 82
Y688
F
A
E
P
Y
N
L
Y
S
S
L
K
E
L
V
Site 83
S690
E
P
Y
N
L
Y
S
S
L
K
E
L
V
L
H
Site 84
Y698
L
K
E
L
V
L
H
Y
Q
H
T
S
L
V
Q
Site 85
S709
S
L
V
Q
H
N
D
S
L
N
V
T
L
A
Y
Site 86
T713
H
N
D
S
L
N
V
T
L
A
Y
P
V
Y
A
Site 87
Y716
S
L
N
V
T
L
A
Y
P
V
Y
A
Q
Q
R
Site 88
Y719
V
T
L
A
Y
P
V
Y
A
Q
Q
R
R
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation