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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
eNOS
Full Name:
Nitric oxide synthase, endothelial
Alias:
CNOS; Constitutive NOS; ECNOS; EC-NOS; Endothelial nitric oxide synthase; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric-oxide synthase, endothelial; NOS type III;NOSIII;Endothelial NOS;eNOS;Constitutive NOS;cNOS; NOS, type III; NOS3; NOSIII
Type:
Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39
Mass (Da):
133289
Number AA:
1203
UniProt ID:
P29474
International Prot ID:
IPI00218845
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0000139
GO:0005901
GO:0005856
Uniprot
OncoNet
Molecular Function:
GO:0050660
GO:0010181
GO:0050661
PhosphoSite+
KinaseNET
Biological Process:
GO:0006916
GO:0006527
GO:0001974
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S6
_
_
M
G
N
L
K
S
V
A
Q
E
P
G
P
Site 2
T33
C
G
K
Q
G
P
A
T
P
A
P
E
P
S
R
Site 3
S39
A
T
P
A
P
E
P
S
R
A
P
A
S
L
L
Site 4
S44
E
P
S
R
A
P
A
S
L
L
P
P
A
P
E
Site 5
S53
L
P
P
A
P
E
H
S
P
P
S
S
P
L
T
Site 6
S56
A
P
E
H
S
P
P
S
S
P
L
T
Q
P
P
Site 7
S57
P
E
H
S
P
P
S
S
P
L
T
Q
P
P
E
Site 8
T60
S
P
P
S
S
P
L
T
Q
P
P
E
G
P
K
Site 9
S78
V
K
N
W
E
V
G
S
I
T
Y
D
T
L
S
Site 10
Y81
W
E
V
G
S
I
T
Y
D
T
L
S
A
Q
A
Site 11
T83
V
G
S
I
T
Y
D
T
L
S
A
Q
A
Q
Q
Site 12
S85
S
I
T
Y
D
T
L
S
A
Q
A
Q
Q
D
G
Site 13
T95
A
Q
Q
D
G
P
C
T
P
R
R
C
L
G
S
Site 14
S102
T
P
R
R
C
L
G
S
L
V
F
P
R
K
L
Site 15
S114
R
K
L
Q
G
R
P
S
P
G
P
P
A
P
E
Site 16
S125
P
A
P
E
Q
L
L
S
Q
A
R
D
F
I
N
Site 17
Y134
A
R
D
F
I
N
Q
Y
Y
S
S
I
K
R
S
Site 18
Y135
R
D
F
I
N
Q
Y
Y
S
S
I
K
R
S
G
Site 19
S136
D
F
I
N
Q
Y
Y
S
S
I
K
R
S
G
S
Site 20
S137
F
I
N
Q
Y
Y
S
S
I
K
R
S
G
S
Q
Site 21
S141
Y
Y
S
S
I
K
R
S
G
S
Q
A
H
E
Q
Site 22
S143
S
S
I
K
R
S
G
S
Q
A
H
E
Q
R
L
Site 23
S168
G
T
Y
Q
L
R
E
S
E
L
V
F
G
A
K
Site 24
Y217
Y
I
C
N
H
I
K
Y
A
T
N
R
G
N
L
Site 25
T219
C
N
H
I
K
Y
A
T
N
R
G
N
L
R
S
Site 26
S226
T
N
R
G
N
L
R
S
A
I
T
V
F
P
Q
Site 27
T229
G
N
L
R
S
A
I
T
V
F
P
Q
R
C
P
Site 28
S260
G
Y
R
Q
Q
D
G
S
V
R
G
D
P
A
N
Site 29
Y357
A
A
P
F
S
G
W
Y
M
S
T
E
I
G
T
Site 30
T387
A
V
C
M
D
L
D
T
R
T
T
S
S
L
W
Site 31
T389
C
M
D
L
D
T
R
T
T
S
S
L
W
K
D
Site 32
T390
M
D
L
D
T
R
T
T
S
S
L
W
K
D
K
Site 33
S391
D
L
D
T
R
T
T
S
S
L
W
K
D
K
A
Site 34
S392
L
D
T
R
T
T
S
S
L
W
K
D
K
A
A
Site 35
T457
P
P
I
S
G
S
L
T
P
V
F
H
Q
E
M
Site 36
Y467
F
H
Q
E
M
V
N
Y
F
L
S
P
A
F
R
Site 37
S470
E
M
V
N
Y
F
L
S
P
A
F
R
Y
Q
P
Site 38
S483
Q
P
D
P
W
K
G
S
A
A
K
G
T
G
I
Site 39
T495
T
G
I
T
R
K
K
T
F
K
E
V
A
N
A
Site 40
S506
V
A
N
A
V
K
I
S
A
S
L
M
G
T
V
Site 41
S508
N
A
V
K
I
S
A
S
L
M
G
T
V
M
A
Site 42
T512
I
S
A
S
L
M
G
T
V
M
A
K
R
V
K
Site 43
Y524
R
V
K
A
T
I
L
Y
G
S
E
T
G
R
A
Site 44
T528
T
I
L
Y
G
S
E
T
G
R
A
Q
S
Y
A
Site 45
S533
S
E
T
G
R
A
Q
S
Y
A
Q
Q
L
G
R
Site 46
Y534
E
T
G
R
A
Q
S
Y
A
Q
Q
L
G
R
L
Site 47
Y556
R
V
L
C
M
D
E
Y
D
V
V
S
L
E
H
Site 48
S560
M
D
E
Y
D
V
V
S
L
E
H
E
T
L
V
Site 49
S585
D
P
P
E
N
G
E
S
F
A
A
A
L
M
E
Site 50
S594
A
A
A
L
M
E
M
S
G
P
Y
N
S
S
P
Site 51
S599
E
M
S
G
P
Y
N
S
S
P
R
P
E
Q
H
Site 52
S600
M
S
G
P
Y
N
S
S
P
R
P
E
Q
H
K
Site 53
S608
P
R
P
E
Q
H
K
S
Y
K
I
R
F
N
S
Site 54
Y609
R
P
E
Q
H
K
S
Y
K
I
R
F
N
S
I
Site 55
S615
S
Y
K
I
R
F
N
S
I
S
C
S
D
P
L
Site 56
S617
K
I
R
F
N
S
I
S
C
S
D
P
L
V
S
Site 57
S619
R
F
N
S
I
S
C
S
D
P
L
V
S
S
W
Site 58
S624
S
C
S
D
P
L
V
S
S
W
R
R
K
R
K
Site 59
S625
C
S
D
P
L
V
S
S
W
R
R
K
R
K
E
Site 60
S633
W
R
R
K
R
K
E
S
S
N
T
D
S
A
G
Site 61
S634
R
R
K
R
K
E
S
S
N
T
D
S
A
G
A
Site 62
T636
K
R
K
E
S
S
N
T
D
S
A
G
A
L
G
Site 63
S638
K
E
S
S
N
T
D
S
A
G
A
L
G
T
L
Site 64
Y657
F
G
L
G
S
R
A
Y
P
H
F
C
A
F
A
Site 65
S725
A
A
A
R
D
I
F
S
P
K
R
S
W
K
R
Site 66
S729
D
I
F
S
P
K
R
S
W
K
R
Q
R
Y
R
Site 67
Y735
R
S
W
K
R
Q
R
Y
R
L
S
A
Q
A
E
Site 68
S738
K
R
Q
R
Y
R
L
S
A
Q
A
E
G
L
Q
Site 69
T762
R
R
K
M
F
Q
A
T
I
R
S
V
E
N
L
Site 70
S765
M
F
Q
A
T
I
R
S
V
E
N
L
Q
S
S
Site 71
S771
R
S
V
E
N
L
Q
S
S
K
S
T
R
A
T
Site 72
S774
E
N
L
Q
S
S
K
S
T
R
A
T
I
L
V
Site 73
T778
S
S
K
S
T
R
A
T
I
L
V
R
L
D
T
Site 74
T785
T
I
L
V
R
L
D
T
G
G
Q
E
G
L
Q
Site 75
Y793
G
G
Q
E
G
L
Q
Y
Q
P
G
D
H
I
G
Site 76
S836
V
E
Q
L
E
K
G
S
P
G
G
P
P
P
G
Site 77
T854
D
P
R
L
P
P
C
T
L
R
Q
A
L
T
F
Site 78
S878
P
Q
L
L
R
L
L
S
T
L
A
E
E
P
R
Site 79
T879
Q
L
L
R
L
L
S
T
L
A
E
E
P
R
E
Site 80
S894
Q
Q
E
L
E
A
L
S
Q
D
P
R
R
Y
E
Site 81
Y900
L
S
Q
D
P
R
R
Y
E
E
W
K
W
F
R
Site 82
Y939
L
P
L
L
Q
P
R
Y
Y
S
V
S
S
A
P
Site 83
Y940
P
L
L
Q
P
R
Y
Y
S
V
S
S
A
P
S
Site 84
S941
L
L
Q
P
R
Y
Y
S
V
S
S
A
P
S
T
Site 85
S943
Q
P
R
Y
Y
S
V
S
S
A
P
S
T
H
P
Site 86
S944
P
R
Y
Y
S
V
S
S
A
P
S
T
H
P
G
Site 87
S947
Y
S
V
S
S
A
P
S
T
H
P
G
E
I
H
Site 88
T948
S
V
S
S
A
P
S
T
H
P
G
E
I
H
L
Site 89
S981
G
V
C
S
T
W
L
S
Q
L
K
P
G
D
P
Site 90
S998
C
F
I
R
G
A
P
S
F
R
L
P
P
D
P
Site 91
S1034
E
R
L
H
D
I
E
S
K
G
L
Q
P
T
P
Site 92
T1040
E
S
K
G
L
Q
P
T
P
M
T
L
V
F
G
Site 93
Y1057
C
S
Q
L
D
H
L
Y
R
D
E
V
Q
N
A
Site 94
T1075
G
V
F
G
R
V
L
T
A
F
S
R
E
P
D
Site 95
S1078
G
R
V
L
T
A
F
S
R
E
P
D
N
P
K
Site 96
T1086
R
E
P
D
N
P
K
T
Y
V
Q
D
I
L
R
Site 97
Y1087
E
P
D
N
P
K
T
Y
V
Q
D
I
L
R
T
Site 98
Y1155
V
L
R
D
Q
Q
R
Y
H
E
D
I
F
G
L
Site 99
T1163
H
E
D
I
F
G
L
T
L
R
T
Q
E
V
T
Site 100
T1170
T
L
R
T
Q
E
V
T
S
R
I
R
T
Q
S
Site 101
S1171
L
R
T
Q
E
V
T
S
R
I
R
T
Q
S
F
Site 102
T1175
E
V
T
S
R
I
R
T
Q
S
F
S
L
Q
E
Site 103
S1177
T
S
R
I
R
T
Q
S
F
S
L
Q
E
R
Q
Site 104
S1179
R
I
R
T
Q
S
F
S
L
Q
E
R
Q
L
R
Site 105
S1198
W
A
F
D
P
P
G
S
D
T
N
S
P
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation