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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
NEK2
Full Name:
Serine/threonine-protein kinase Nek2
Alias:
EC 2.7.11.1; HSPK 21; Kinase Nek2; NEK2A; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1
Type:
Protein kinase, tyrosine (non-receptor); Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Other group; NEK family
Mass (Da):
51763
Number AA:
445
UniProt ID:
P51955
International Prot ID:
IPI00021331
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005813
GO:0005829
GO:0005730
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0000287
GO:0019903
PhosphoSite+
KinaseNET
Biological Process:
GO:0051301
GO:0051299
GO:0007126
Phosida
TranscriptoNet
STRING
Kinexus Products
NIMA (never-in-mitosis)-related protein-serine kinase 2 S171 phosphosite-specific antibody AB-PK732#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK732#NIMA (never-in-mitosis)-related protein-serine kinase 2 T170+S171 phosphosite-specific antibody AB-PK733#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK733#NIMA (never-in-mitosis)-related protein-serine kinase 2 (H168-K174, human) pT170+pS171 phosphopeptide - Powder PE-04ABO90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ABO90#NIMA (never-in-mitosis)-related protein-serine kinase 2 (H168-K174, human) pS171 phosphopeptide - Powder PE-04ABP99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ABP99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y8
M
P
S
R
A
E
D
Y
E
V
L
Y
T
I
G
Site 2
Y12
A
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
Site 3
T13
E
D
Y
E
V
L
Y
T
I
G
T
G
S
Y
G
Site 4
S18
L
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
Site 5
Y19
Y
T
I
G
T
G
S
Y
G
R
C
Q
K
I
R
Site 6
S29
C
Q
K
I
R
R
K
S
D
G
K
I
L
V
W
Site 7
Y41
L
V
W
K
E
L
D
Y
G
S
M
T
E
A
E
Site 8
S43
W
K
E
L
D
Y
G
S
M
T
E
A
E
K
Q
Site 9
Y70
K
H
P
N
I
V
R
Y
Y
D
R
I
I
D
R
Site 10
Y71
H
P
N
I
V
R
Y
Y
D
R
I
I
D
R
T
Site 11
T78
Y
D
R
I
I
D
R
T
N
T
T
L
Y
I
V
Site 12
T80
R
I
I
D
R
T
N
T
T
L
Y
I
V
M
E
Site 13
Y83
D
R
T
N
T
T
L
Y
I
V
M
E
Y
C
E
Site 14
S96
C
E
G
G
D
L
A
S
V
I
T
K
G
T
K
Site 15
Y107
K
G
T
K
E
R
Q
Y
L
D
E
E
F
V
L
Site 16
S131
L
K
E
C
H
R
R
S
D
G
G
H
T
V
L
Site 17
T136
R
R
S
D
G
G
H
T
V
L
H
R
D
L
K
Site 18
T170
A
R
I
L
N
H
D
T
S
F
A
K
T
F
V
NIMA (never-in-mitosis)-related protein-serine kinase 2 T170+S171 phosphosite-specific antibody AB-PK733#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK733#NIMA (never-in-mitosis)-related protein-serine kinase 2 (H168-K174, human) pT170+pS171 phosphopeptide - Powder PE-04ABO90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ABO90
Site 19
S171
R
I
L
N
H
D
T
S
F
A
K
T
F
V
G
NIMA (never-in-mitosis)-related protein-serine kinase 2 S171 phosphosite-specific antibody AB-PK732#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK732#NIMA (never-in-mitosis)-related protein-serine kinase 2 T170+S171 phosphosite-specific antibody AB-PK733#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK733#NIMA (never-in-mitosis)-related protein-serine kinase 2 (H168-K174, human) pT170+pS171 phosphopeptide - Powder PE-04ABO90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ABO90#NIMA (never-in-mitosis)-related protein-serine kinase 2 (H168-K174, human) pS171 phosphopeptide - Powder PE-04ABP99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04ABP99
Site 20
T175
H
D
T
S
F
A
K
T
F
V
G
T
P
Y
Y
Site 21
T179
F
A
K
T
F
V
G
T
P
Y
Y
M
S
P
E
Site 22
Y181
K
T
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
Site 23
Y182
T
F
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
Site 24
S184
V
G
T
P
Y
Y
M
S
P
E
Q
M
N
R
M
Site 25
S192
P
E
Q
M
N
R
M
S
Y
N
E
K
S
D
I
Site 26
Y193
E
Q
M
N
R
M
S
Y
N
E
K
S
D
I
W
Site 27
S201
N
E
K
S
D
I
W
S
L
G
C
L
L
Y
E
Site 28
S220
M
P
P
F
T
A
F
S
Q
K
E
L
A
G
K
Site 29
Y238
G
K
F
R
R
I
P
Y
R
Y
S
D
E
L
N
Site 30
Y240
F
R
R
I
P
Y
R
Y
S
D
E
L
N
E
I
Site 31
S241
R
R
I
P
Y
R
Y
S
D
E
L
N
E
I
I
Site 32
Y257
R
M
L
N
L
K
D
Y
H
R
P
S
V
E
E
Site 33
S261
L
K
D
Y
H
R
P
S
V
E
E
I
L
E
N
Site 34
S296
Q
L
G
E
P
E
K
S
Q
D
S
S
P
V
L
Site 35
S299
E
P
E
K
S
Q
D
S
S
P
V
L
S
E
L
Site 36
S300
P
E
K
S
Q
D
S
S
P
V
L
S
E
L
K
Site 37
S304
Q
D
S
S
P
V
L
S
E
L
K
L
K
E
I
Site 38
S356
E
N
L
L
K
N
Y
S
L
L
K
E
R
K
F
Site 39
S365
L
K
E
R
K
F
L
S
L
A
S
N
P
E
L
Site 40
S368
R
K
F
L
S
L
A
S
N
P
E
L
L
N
L
Site 41
S377
P
E
L
L
N
L
P
S
S
V
I
K
K
K
V
Site 42
S378
E
L
L
N
L
P
S
S
V
I
K
K
K
V
H
Site 43
S387
I
K
K
K
V
H
F
S
G
E
S
K
E
N
I
Site 44
S397
S
K
E
N
I
M
R
S
E
N
S
E
S
Q
L
Site 45
S400
N
I
M
R
S
E
N
S
E
S
Q
L
T
S
K
Site 46
S402
M
R
S
E
N
S
E
S
Q
L
T
S
K
S
K
Site 47
T405
E
N
S
E
S
Q
L
T
S
K
S
K
C
K
D
Site 48
S406
N
S
E
S
Q
L
T
S
K
S
K
C
K
D
L
Site 49
S408
E
S
Q
L
T
S
K
S
K
C
K
D
L
K
K
Site 50
S428
Q
L
R
A
Q
A
L
S
D
I
E
K
N
Y
Q
Site 51
Y434
L
S
D
I
E
K
N
Y
Q
L
K
S
R
Q
I
Site 52
S438
E
K
N
Y
Q
L
K
S
R
Q
I
L
G
M
R
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation