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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
TOP2B
Full Name:
DNA topoisomerase 2-beta
Alias:
EC 5.99.1.3; Topoisomerase (DNA) II beta 180kDa; TP2B; TPB2
Type:
Topoisomerase; EC 5.99.1.3; Cell development/differentiation; Motility/polarity/chemotaxis; DNA binding protein
Mass (Da):
183267
Number AA:
1626
UniProt ID:
Q02880
International Prot ID:
IPI00027280
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0000785
GO:0000792
GO:0005634
Uniprot
OncoNet
Molecular Function:
GO:0000166
GO:0003676
GO:0003677
PhosphoSite+
KinaseNET
Biological Process:
GO:0001764
GO:0006139
GO:0006259
Phosida
TranscriptoNet
STRING
Kinexus Products
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S37
N
A
A
K
K
E
E
S
E
T
A
N
K
N
D
Site 2
T39
A
K
K
E
E
S
E
T
A
N
K
N
D
S
S
Site 3
S45
E
T
A
N
K
N
D
S
S
K
K
L
S
V
E
Site 4
S46
T
A
N
K
N
D
S
S
K
K
L
S
V
E
R
Site 5
S50
N
D
S
S
K
K
L
S
V
E
R
V
Y
Q
K
Site 6
Y55
K
L
S
V
E
R
V
Y
Q
K
K
T
Q
L
E
Site 7
T70
H
I
L
L
R
P
D
T
Y
I
G
S
V
E
P
Site 8
S74
R
P
D
T
Y
I
G
S
V
E
P
L
T
Q
F
Site 9
Y85
L
T
Q
F
M
W
V
Y
D
E
D
V
G
M
N
Site 10
T97
G
M
N
C
R
E
V
T
F
V
P
G
L
Y
K
Site 11
Y103
V
T
F
V
P
G
L
Y
K
I
F
D
E
I
L
Site 12
T124
K
Q
R
D
K
N
M
T
C
I
K
V
S
I
D
Site 13
Y157
E
H
K
V
E
K
V
Y
V
P
A
L
I
F
G
Site 14
S170
F
G
Q
L
L
T
S
S
N
Y
D
D
D
E
K
Site 15
T180
D
D
D
E
K
K
V
T
G
G
R
N
G
Y
G
Site 16
Y186
V
T
G
G
R
N
G
Y
G
A
K
L
C
N
I
Site 17
T199
N
I
F
S
T
K
F
T
V
E
T
A
C
K
E
Site 18
Y235
K
H
F
D
G
E
D
Y
T
C
I
T
F
Q
P
Site 19
T239
G
E
D
Y
T
C
I
T
F
Q
P
D
L
S
K
Site 20
S245
I
T
F
Q
P
D
L
S
K
F
K
M
E
K
L
Site 21
Y265
A
L
M
T
R
R
A
Y
D
L
A
G
S
C
R
Site 22
S270
R
A
Y
D
L
A
G
S
C
R
G
V
K
V
M
Site 23
Y291
P
V
N
G
F
R
S
Y
V
D
L
Y
V
K
D
Site 24
Y295
F
R
S
Y
V
D
L
Y
V
K
D
K
L
D
E
Site 25
T323
E
R
W
D
V
C
L
T
L
S
E
K
G
F
Q
Site 26
S333
E
K
G
F
Q
Q
I
S
F
V
N
S
I
A
T
Site 27
S337
Q
Q
I
S
F
V
N
S
I
A
T
T
K
G
G
Site 28
Y349
K
G
G
R
H
V
D
Y
V
V
D
Q
V
V
G
Site 29
S371
K
K
N
K
A
G
V
S
V
K
P
F
Q
V
K
Site 30
T393
N
C
L
I
E
N
P
T
F
D
S
Q
T
K
E
Site 31
S396
I
E
N
P
T
F
D
S
Q
T
K
E
N
M
T
Site 32
T403
S
Q
T
K
E
N
M
T
L
Q
P
K
S
F
G
Site 33
S408
N
M
T
L
Q
P
K
S
F
G
S
K
C
Q
L
Site 34
S411
L
Q
P
K
S
F
G
S
K
C
Q
L
S
E
K
Site 35
S416
F
G
S
K
C
Q
L
S
E
K
F
F
K
A
A
Site 36
S424
E
K
F
F
K
A
A
S
N
C
G
I
V
E
S
Site 37
S431
S
N
C
G
I
V
E
S
I
L
N
W
V
K
F
Site 38
S449
T
Q
L
N
K
K
C
S
S
V
K
Y
S
K
I
Site 39
S450
Q
L
N
K
K
C
S
S
V
K
Y
S
K
I
K
Site 40
Y453
K
K
C
S
S
V
K
Y
S
K
I
K
G
I
P
Site 41
S454
K
C
S
S
V
K
Y
S
K
I
K
G
I
P
K
Site 42
S473
N
D
A
G
G
K
H
S
L
E
C
T
L
I
L
Site 43
T477
G
K
H
S
L
E
C
T
L
I
L
T
E
G
D
Site 44
S488
T
E
G
D
S
A
K
S
L
A
V
S
G
L
G
Site 45
S492
S
A
K
S
L
A
V
S
G
L
G
V
I
G
R
Site 46
Y502
G
V
I
G
R
D
R
Y
G
V
F
P
L
R
G
Site 47
S518
I
L
N
V
R
E
A
S
H
K
Q
I
M
E
N
Site 48
Y539
I
K
I
V
G
L
Q
Y
K
K
S
Y
D
D
A
Site 49
S542
V
G
L
Q
Y
K
K
S
Y
D
D
A
E
S
L
Site 50
S548
K
S
Y
D
D
A
E
S
L
K
T
L
R
Y
G
Site 51
T551
D
D
A
E
S
L
K
T
L
R
Y
G
K
I
M
Site 52
Y554
E
S
L
K
T
L
R
Y
G
K
I
M
I
M
T
Site 53
S568
T
D
Q
D
Q
D
G
S
H
I
K
G
L
L
I
Site 54
S609
S
K
N
K
Q
E
L
S
F
Y
S
I
P
E
F
Site 55
Y611
N
K
Q
E
L
S
F
Y
S
I
P
E
F
D
E
Site 56
S612
K
Q
E
L
S
F
Y
S
I
P
E
F
D
E
W
Site 57
Y633
Q
K
A
W
K
I
K
Y
Y
K
G
L
G
T
S
Site 58
Y634
K
A
W
K
I
K
Y
Y
K
G
L
G
T
S
T
Site 59
T639
K
Y
Y
K
G
L
G
T
S
T
A
K
E
A
K
Site 60
S640
Y
Y
K
G
L
G
T
S
T
A
K
E
A
K
E
Site 61
Y648
T
A
K
E
A
K
E
Y
F
A
D
M
E
R
H
Site 62
Y661
R
H
R
I
L
F
R
Y
A
G
P
E
D
D
A
Site 63
T671
P
E
D
D
A
A
I
T
L
A
F
S
K
K
K
Site 64
Y707
G
L
P
E
Q
F
L
Y
G
T
A
T
K
H
L
Site 65
T715
G
T
A
T
K
H
L
T
Y
N
D
F
I
N
K
Site 66
S728
N
K
E
L
I
L
F
S
N
S
D
N
E
R
S
Site 67
S730
E
L
I
L
F
S
N
S
D
N
E
R
S
I
P
Site 68
S735
S
N
S
D
N
E
R
S
I
P
S
L
V
D
G
Site 69
S738
D
N
E
R
S
I
P
S
L
V
D
G
F
K
P
Site 70
T753
G
Q
R
K
V
L
F
T
C
F
K
R
N
D
K
Site 71
S776
A
G
S
V
A
E
M
S
A
Y
H
H
G
E
Q
Site 72
Y778
S
V
A
E
M
S
A
Y
H
H
G
E
Q
A
L
Site 73
S823
H
G
G
K
D
A
A
S
P
R
Y
I
F
T
M
Site 74
Y826
K
D
A
A
S
P
R
Y
I
F
T
M
L
S
T
Site 75
Y851
D
N
L
L
K
F
L
Y
D
D
N
Q
R
V
E
Site 76
T879
N
G
A
E
G
I
G
T
G
W
A
C
K
L
P
Site 77
Y888
W
A
C
K
L
P
N
Y
D
A
R
E
I
V
N
Site 78
Y913
P
H
P
M
L
P
N
Y
K
N
F
K
G
T
I
Site 79
Y928
Q
E
L
G
Q
N
Q
Y
A
V
S
G
E
I
F
Site 80
T953
E
L
P
V
R
T
W
T
Q
V
Y
K
E
Q
V
Site 81
T971
M
L
N
G
T
D
K
T
P
A
L
I
S
D
Y
Site 82
S976
D
K
T
P
A
L
I
S
D
Y
K
E
Y
H
T
Site 83
Y978
T
P
A
L
I
S
D
Y
K
E
Y
H
T
D
T
Site 84
T985
Y
K
E
Y
H
T
D
T
T
V
K
F
V
V
K
Site 85
T986
K
E
Y
H
T
D
T
T
V
K
F
V
V
K
M
Site 86
Y1033
H
M
G
C
L
K
K
Y
E
T
V
Q
D
I
L
Site 87
T1035
G
C
L
K
K
Y
E
T
V
Q
D
I
L
K
E
Site 88
S1049
E
F
F
D
L
R
L
S
Y
Y
G
L
R
K
E
Site 89
Y1050
F
F
D
L
R
L
S
Y
Y
G
L
R
K
E
W
Site 90
Y1051
F
D
L
R
L
S
Y
Y
G
L
R
K
E
W
L
Site 91
S1090
K
I
T
I
E
N
R
S
K
K
D
L
I
Q
M
Site 92
Y1103
Q
M
L
V
Q
R
G
Y
E
S
D
P
V
K
A
Site 93
T1124
K
A
A
E
E
D
E
T
Q
N
Q
H
D
D
S
Site 94
S1131
T
Q
N
Q
H
D
D
S
S
S
D
S
G
T
P
Site 95
S1132
Q
N
Q
H
D
D
S
S
S
D
S
G
T
P
S
Site 96
S1133
N
Q
H
D
D
S
S
S
D
S
G
T
P
S
G
Site 97
S1135
H
D
D
S
S
S
D
S
G
T
P
S
G
P
D
Site 98
T1137
D
S
S
S
D
S
G
T
P
S
G
P
D
F
N
Site 99
S1139
S
S
D
S
G
T
P
S
G
P
D
F
N
Y
I
Site 100
Y1145
P
S
G
P
D
F
N
Y
I
L
N
M
S
L
W
Site 101
S1180
V
N
D
L
K
R
K
S
P
S
D
L
W
K
E
Site 102
S1182
D
L
K
R
K
S
P
S
D
L
W
K
E
D
L
Site 103
S1201
E
E
L
D
K
V
E
S
Q
E
R
E
D
V
L
Site 104
S1212
E
D
V
L
A
G
M
S
G
K
A
I
K
G
K
Site 105
T1233
K
K
L
Q
L
E
E
T
M
P
S
P
Y
G
R
Site 106
S1236
Q
L
E
E
T
M
P
S
P
Y
G
R
R
I
I
Site 107
Y1238
E
E
T
M
P
S
P
Y
G
R
R
I
I
P
E
Site 108
T1267
K
K
K
G
D
L
D
T
A
A
V
K
V
E
F
Site 109
T1292
G
A
G
E
E
A
L
T
P
S
V
P
I
N
K
Site 110
S1294
G
E
E
A
L
T
P
S
V
P
I
N
K
G
P
Site 111
T1312
R
E
K
K
E
P
G
T
R
V
R
K
T
P
T
Site 112
T1317
P
G
T
R
V
R
K
T
P
T
S
S
G
K
P
Site 113
T1319
T
R
V
R
K
T
P
T
S
S
G
K
P
S
A
Site 114
S1320
R
V
R
K
T
P
T
S
S
G
K
P
S
A
K
Site 115
S1321
V
R
K
T
P
T
S
S
G
K
P
S
A
K
K
Site 116
S1325
P
T
S
S
G
K
P
S
A
K
K
V
K
K
R
Site 117
S1336
V
K
K
R
N
P
W
S
D
D
E
S
K
S
E
Site 118
S1340
N
P
W
S
D
D
E
S
K
S
E
S
D
L
E
Site 119
S1342
W
S
D
D
E
S
K
S
E
S
D
L
E
E
T
Site 120
S1344
D
D
E
S
K
S
E
S
D
L
E
E
T
E
P
Site 121
S1358
P
V
V
I
P
R
D
S
L
L
R
R
A
A
A
Site 122
Y1370
A
A
A
E
R
P
K
Y
T
F
D
F
S
E
E
Site 123
T1371
A
A
E
R
P
K
Y
T
F
D
F
S
E
E
E
Site 124
S1375
P
K
Y
T
F
D
F
S
E
E
E
D
D
D
A
Site 125
S1400
E
E
L
K
V
K
A
S
P
I
T
N
D
G
E
Site 126
T1403
K
V
K
A
S
P
I
T
N
D
G
E
D
E
F
Site 127
S1413
G
E
D
E
F
V
P
S
D
G
L
D
K
D
E
Site 128
Y1421
D
G
L
D
K
D
E
Y
T
F
S
P
G
K
S
Site 129
T1422
G
L
D
K
D
E
Y
T
F
S
P
G
K
S
K
Site 130
S1424
D
K
D
E
Y
T
F
S
P
G
K
S
K
A
T
Site 131
S1428
Y
T
F
S
P
G
K
S
K
A
T
P
E
K
S
Site 132
T1431
S
P
G
K
S
K
A
T
P
E
K
S
L
H
D
Site 133
S1435
S
K
A
T
P
E
K
S
L
H
D
K
K
S
Q
Site 134
S1441
K
S
L
H
D
K
K
S
Q
D
F
G
N
L
F
Site 135
S1449
Q
D
F
G
N
L
F
S
F
P
S
Y
S
Q
K
Site 136
S1452
G
N
L
F
S
F
P
S
Y
S
Q
K
S
E
D
Site 137
Y1453
N
L
F
S
F
P
S
Y
S
Q
K
S
E
D
D
Site 138
S1454
L
F
S
F
P
S
Y
S
Q
K
S
E
D
D
S
Site 139
S1457
F
P
S
Y
S
Q
K
S
E
D
D
S
A
K
F
Site 140
S1461
S
Q
K
S
E
D
D
S
A
K
F
D
S
N
E
Site 141
S1466
D
D
S
A
K
F
D
S
N
E
E
D
S
A
S
Site 142
S1471
F
D
S
N
E
E
D
S
A
S
V
F
S
P
S
Site 143
S1473
S
N
E
E
D
S
A
S
V
F
S
P
S
F
G
Site 144
S1476
E
D
S
A
S
V
F
S
P
S
F
G
L
K
Q
Site 145
S1478
S
A
S
V
F
S
P
S
F
G
L
K
Q
T
D
Site 146
T1491
T
D
K
V
P
S
K
T
V
A
A
K
K
G
K
Site 147
S1500
A
A
K
K
G
K
P
S
S
D
T
V
P
K
P
Site 148
S1501
A
K
K
G
K
P
S
S
D
T
V
P
K
P
K
Site 149
T1503
K
G
K
P
S
S
D
T
V
P
K
P
K
R
A
Site 150
S1522
K
V
V
E
A
V
N
S
D
S
D
S
E
F
G
Site 151
S1524
V
E
A
V
N
S
D
S
D
S
E
F
G
I
P
Site 152
S1526
A
V
N
S
D
S
D
S
E
F
G
I
P
K
K
Site 153
T1534
E
F
G
I
P
K
K
T
T
T
P
K
G
K
G
Site 154
T1536
G
I
P
K
K
T
T
T
P
K
G
K
G
R
G
Site 155
S1550
G
A
K
K
R
K
A
S
G
S
E
N
E
G
D
Site 156
S1552
K
K
R
K
A
S
G
S
E
N
E
G
D
Y
N
Site 157
Y1558
G
S
E
N
E
G
D
Y
N
P
G
R
K
T
S
Site 158
T1564
D
Y
N
P
G
R
K
T
S
K
T
T
S
K
K
Site 159
S1565
Y
N
P
G
R
K
T
S
K
T
T
S
K
K
P
Site 160
T1567
P
G
R
K
T
S
K
T
T
S
K
K
P
K
K
Site 161
S1569
R
K
T
S
K
T
T
S
K
K
P
K
K
T
S
Site 162
T1575
T
S
K
K
P
K
K
T
S
F
D
Q
D
S
D
Site 163
S1576
S
K
K
P
K
K
T
S
F
D
Q
D
S
D
V
Site 164
S1581
K
T
S
F
D
Q
D
S
D
V
D
I
F
P
S
Site 165
S1588
S
D
V
D
I
F
P
S
D
F
P
T
E
P
P
Site 166
T1592
I
F
P
S
D
F
P
T
E
P
P
S
L
P
R
Site 167
S1596
D
F
P
T
E
P
P
S
L
P
R
T
G
R
A
Site 168
T1600
E
P
P
S
L
P
R
T
G
R
A
R
K
E
V
Site 169
Y1609
R
A
R
K
E
V
K
Y
F
A
E
S
D
E
E
Site 170
S1613
E
V
K
Y
F
A
E
S
D
E
E
E
D
D
V
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation