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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
ROCK1
Full Name:
Rho-associated protein kinase 1
Alias:
Renal carcinoma antigen NY-REN-35;Rho-associated, coiled-coil-containing protein kinase 1;p160 ROCK-1
Type:
Mass (Da):
158157
Number AA:
1354
UniProt ID:
Q13464
International Prot ID:
IPI00641659
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005622
GO:0044464
GO:0000139
Uniprot
OncoNet
Molecular Function:
GO:0000166
GO:0003824
GO:0004672
PhosphoSite+
KinaseNET
Biological Process:
GO:0000910
GO:0006464
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
Rho-associated protein kinase 1 Y913 phosphosite-specific antibody AB-PK798#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK798#Rock1Subtide - ROCK1 protein kinase substrate peptide - Powder PE-01BJD99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-01BJD99#Rho-associated protein kinase 1 (E910-L916, human) pY913 phosphopeptide - Powder PE-04APL99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APL99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S6
_
_
M
S
T
G
D
S
F
E
T
R
F
E
K
Site 2
S23
N
L
L
R
D
P
K
S
E
V
N
S
D
C
L
Site 3
S27
D
P
K
S
E
V
N
S
D
C
L
L
D
G
L
Site 4
Y76
L
R
M
K
A
E
D
Y
E
V
V
K
V
I
G
Site 5
S97
V
Q
L
V
R
H
K
S
T
R
K
V
Y
A
M
Site 6
Y102
H
K
S
T
R
K
V
Y
A
M
K
L
L
S
K
Site 7
S118
E
M
I
K
R
S
D
S
A
F
F
W
E
E
R
Site 8
Y148
Y
A
F
Q
D
D
R
Y
L
Y
M
V
M
E
Y
Site 9
Y150
F
Q
D
D
R
Y
L
Y
M
V
M
E
Y
M
P
Site 10
S166
G
D
L
V
N
L
M
S
N
Y
D
V
P
E
K
Site 11
Y168
L
V
N
L
M
S
N
Y
D
V
P
E
K
W
A
Site 12
Y178
P
E
K
W
A
R
F
Y
T
A
E
V
V
L
A
Site 13
S209
D
N
M
L
L
D
K
S
G
H
L
K
L
A
D
Site 14
T219
L
K
L
A
D
F
G
T
C
M
K
M
N
K
E
Site 15
T233
E
G
M
V
R
C
D
T
A
V
G
T
P
D
Y
Site 16
T237
R
C
D
T
A
V
G
T
P
D
Y
I
S
P
E
Site 17
Y240
T
A
V
G
T
P
D
Y
I
S
P
E
V
L
K
Site 18
S242
V
G
T
P
D
Y
I
S
P
E
V
L
K
S
Q
Site 19
S248
I
S
P
E
V
L
K
S
Q
G
G
D
G
Y
Y
Site 20
Y254
K
S
Q
G
G
D
G
Y
Y
G
R
E
C
D
W
Site 21
Y255
S
Q
G
G
D
G
Y
Y
G
R
E
C
D
W
W
Site 22
S282
D
T
P
F
Y
A
D
S
L
V
G
T
Y
S
K
Site 23
T286
Y
A
D
S
L
V
G
T
Y
S
K
I
M
N
H
Site 24
T298
M
N
H
K
N
S
L
T
F
P
D
D
N
D
I
Site 25
T351
A
W
E
T
L
R
D
T
V
A
P
V
V
P
D
Site 26
S360
A
P
V
V
P
D
L
S
S
D
I
D
T
S
N
Site 27
S361
P
V
V
P
D
L
S
S
D
I
D
T
S
N
F
Site 28
T365
D
L
S
S
D
I
D
T
S
N
F
D
D
L
E
Site 29
S366
L
S
S
D
I
D
T
S
N
F
D
D
L
E
E
Site 30
Y399
L
P
F
V
G
F
T
Y
Y
S
N
R
R
Y
L
Site 31
Y400
P
F
V
G
F
T
Y
Y
S
N
R
R
Y
L
S
Site 32
S401
F
V
G
F
T
Y
Y
S
N
R
R
Y
L
S
S
Site 33
Y405
T
Y
Y
S
N
R
R
Y
L
S
S
A
N
P
N
Site 34
S407
Y
S
N
R
R
Y
L
S
S
A
N
P
N
D
N
Site 35
S408
S
N
R
R
Y
L
S
S
A
N
P
N
D
N
R
Site 36
T416
A
N
P
N
D
N
R
T
S
S
N
A
D
K
S
Site 37
S417
N
P
N
D
N
R
T
S
S
N
A
D
K
S
L
Site 38
S418
P
N
D
N
R
T
S
S
N
A
D
K
S
L
Q
Site 39
S423
T
S
S
N
A
D
K
S
L
Q
E
S
L
Q
K
Site 40
S427
A
D
K
S
L
Q
E
S
L
Q
K
T
I
Y
K
Site 41
T431
L
Q
E
S
L
Q
K
T
I
Y
K
L
E
E
Q
Site 42
Y433
E
S
L
Q
K
T
I
Y
K
L
E
E
Q
L
H
Site 43
T455
E
M
E
Q
K
C
R
T
S
N
I
K
L
D
K
Site 44
S456
M
E
Q
K
C
R
T
S
N
I
K
L
D
K
I
Site 45
S479
N
Q
R
R
N
L
E
S
T
V
S
Q
I
E
K
Site 46
T480
Q
R
R
N
L
E
S
T
V
S
Q
I
E
K
E
Site 47
S482
R
N
L
E
S
T
V
S
Q
I
E
K
E
K
M
Site 48
Y498
L
Q
H
R
I
N
E
Y
Q
R
K
A
E
Q
E
Site 49
S517
R
N
V
E
N
E
V
S
T
L
K
D
Q
L
E
Site 50
S530
L
E
D
L
K
K
V
S
Q
N
S
Q
L
A
N
Site 51
S533
L
K
K
V
S
Q
N
S
Q
L
A
N
E
K
L
Site 52
S558
N
D
L
L
R
T
E
S
D
T
A
V
R
L
R
Site 53
T560
L
L
R
T
E
S
D
T
A
V
R
L
R
K
S
Site 54
S567
T
A
V
R
L
R
K
S
H
T
E
M
S
K
S
Site 55
T569
V
R
L
R
K
S
H
T
E
M
S
K
S
I
S
Site 56
S572
R
K
S
H
T
E
M
S
K
S
I
S
Q
L
E
Site 57
S574
S
H
T
E
M
S
K
S
I
S
Q
L
E
S
L
Site 58
S576
T
E
M
S
K
S
I
S
Q
L
E
S
L
N
R
Site 59
S580
K
S
I
S
Q
L
E
S
L
N
R
E
L
Q
E
Site 60
S595
R
N
R
I
L
E
N
S
K
S
Q
T
D
K
D
Site 61
S597
R
I
L
E
N
S
K
S
Q
T
D
K
D
Y
Y
Site 62
T599
L
E
N
S
K
S
Q
T
D
K
D
Y
Y
Q
L
Site 63
Y603
K
S
Q
T
D
K
D
Y
Y
Q
L
Q
A
I
L
Site 64
Y604
S
Q
T
D
K
D
Y
Y
Q
L
Q
A
I
L
E
Site 65
S621
R
R
D
R
G
H
D
S
E
M
I
G
D
L
Q
Site 66
T632
G
D
L
Q
A
R
I
T
S
L
Q
E
E
V
K
Site 67
S633
D
L
Q
A
R
I
T
S
L
Q
E
E
V
K
H
Site 68
Y675
N
L
E
I
D
L
N
Y
K
L
K
S
L
Q
Q
Site 69
S679
D
L
N
Y
K
L
K
S
L
Q
Q
R
L
E
Q
Site 70
T694
E
V
N
E
H
K
V
T
K
A
R
L
T
D
K
Site 71
T699
K
V
T
K
A
R
L
T
D
K
H
Q
S
I
E
Site 72
S710
Q
S
I
E
E
A
K
S
V
A
M
C
E
M
E
Site 73
S751
L
D
V
D
L
K
Q
S
Q
Q
K
L
E
H
L
Site 74
Y851
D
Q
L
E
A
E
Q
Y
F
S
T
L
Y
K
T
Site 75
S853
L
E
A
E
Q
Y
F
S
T
L
Y
K
T
Q
V
Site 76
Y856
E
Q
Y
F
S
T
L
Y
K
T
Q
V
K
E
L
Site 77
T858
Y
F
S
T
L
Y
K
T
Q
V
K
E
L
K
E
Site 78
T887
E
L
Q
N
E
K
E
T
L
A
T
Q
L
D
L
Site 79
T890
N
E
K
E
T
L
A
T
Q
L
D
L
A
E
T
Site 80
T897
T
Q
L
D
L
A
E
T
K
A
E
S
E
Q
L
Site 81
Y913
R
G
L
L
E
E
Q
Y
F
E
L
T
Q
E
S
Rho-associated protein kinase 1 Y913 phosphosite-specific antibody AB-PK798#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK798#Rho-associated protein kinase 1 (E910-L916, human) pY913 phosphopeptide - Powder PE-04APL99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04APL99
Site 82
T937
E
I
T
D
K
D
H
T
V
S
R
L
E
E
A
Site 83
S946
S
R
L
E
E
A
N
S
M
L
T
K
D
I
E
Site 84
T963
R
R
E
N
E
E
L
T
E
K
M
K
K
A
E
Site 85
T1024
I
D
R
K
K
A
N
T
Q
D
L
R
K
K
E
Site 86
S1098
R
A
K
L
L
D
L
S
D
S
T
S
V
A
S
Site 87
S1100
K
L
L
D
L
S
D
S
T
S
V
A
S
F
P
Site 88
T1101
L
L
D
L
S
D
S
T
S
V
A
S
F
P
S
Site 89
S1102
L
D
L
S
D
S
T
S
V
A
S
F
P
S
A
Site 90
S1105
S
D
S
T
S
V
A
S
F
P
S
A
D
E
T
Site 91
S1108
T
S
V
A
S
F
P
S
A
D
E
T
D
G
N
Site 92
T1112
S
F
P
S
A
D
E
T
D
G
N
L
P
E
S
Site 93
S1119
T
D
G
N
L
P
E
S
R
I
E
G
W
L
S
Site 94
S1126
S
R
I
E
G
W
L
S
V
P
N
R
G
N
I
Site 95
Y1142
R
Y
G
W
K
K
Q
Y
V
V
V
S
S
K
K
Site 96
Y1153
S
S
K
K
I
L
F
Y
N
D
E
Q
D
K
E
Site 97
T1180
L
F
H
V
R
P
V
T
Q
G
D
V
Y
R
A
Site 98
Y1185
P
V
T
Q
G
D
V
Y
R
A
E
T
E
E
I
Site 99
T1189
G
D
V
Y
R
A
E
T
E
E
I
P
K
I
F
Site 100
Y1200
P
K
I
F
Q
I
L
Y
A
N
E
G
E
C
R
Site 101
T1234
K
G
H
E
F
I
P
T
L
Y
H
F
P
A
N
Site 102
Y1287
I
C
P
C
K
V
S
Y
D
V
T
S
A
R
D
Site 103
S1301
D
M
L
L
L
A
C
S
Q
D
E
Q
K
K
W
Site 104
S1322
K
I
P
K
N
P
P
S
G
F
V
R
A
S
P
Site 105
S1328
P
S
G
F
V
R
A
S
P
R
T
L
S
T
R
Site 106
T1331
F
V
R
A
S
P
R
T
L
S
T
R
S
T
A
Site 107
S1333
R
A
S
P
R
T
L
S
T
R
S
T
A
N
Q
Site 108
T1334
A
S
P
R
T
L
S
T
R
S
T
A
N
Q
S
Site 109
S1336
P
R
T
L
S
T
R
S
T
A
N
Q
S
F
R
Site 110
T1337
R
T
L
S
T
R
S
T
A
N
Q
S
F
R
K
Site 111
S1341
T
R
S
T
A
N
Q
S
F
R
K
V
V
K
N
Site 112
S1350
R
K
V
V
K
N
T
S
G
K
T
S
_
_
_
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation