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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
NKCC2
Full Name:
Solute carrier family 12 member 1
Alias:
Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; Kidney-specific Na-K-Cl symporter; S12A1; SLC12A1; Solute carrier family 12 (sodium/potassium/chloride transporters) member 1
Type:
Transport protein, facilitator
Mass (Da):
121450
Number AA:
1099
UniProt ID:
Q13621
International Prot ID:
IPI00443898
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0016021
GO:0005624
GO:0005886
Uniprot
OncoNet
Molecular Function:
GO:0030955
GO:0031402
GO:0008511
PhosphoSite+
KinaseNET
Biological Process:
GO:0006821
GO:0006813
GO:0006814
Phosida
TranscriptoNet
STRING
Kinexus Products
Solute carrier family 12 member 1 pan-specific antibody AB-NN320-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NN320-1
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
S6
_
_
M
S
L
N
N
S
S
N
V
F
L
D
S
Site 2
S13
S
S
N
V
F
L
D
S
V
P
S
N
T
N
R
Site 3
S16
V
F
L
D
S
V
P
S
N
T
N
R
F
Q
V
Site 4
T18
L
D
S
V
P
S
N
T
N
R
F
Q
V
S
V
Site 5
S32
V
I
N
E
N
H
E
S
S
A
A
A
D
D
N
Site 6
T40
S
A
A
A
D
D
N
T
D
P
P
H
Y
E
E
Site 7
Y45
D
N
T
D
P
P
H
Y
E
E
T
S
F
G
D
Site 8
S49
P
P
H
Y
E
E
T
S
F
G
D
E
A
Q
K
Site 9
S61
A
Q
K
R
L
R
I
S
F
R
P
G
N
Q
E
Site 10
Y70
R
P
G
N
Q
E
C
Y
D
N
F
L
Q
S
G
Site 11
T82
Q
S
G
E
T
A
K
T
D
A
S
F
H
A
Y
Site 12
S85
E
T
A
K
T
D
A
S
F
H
A
Y
D
S
H
Site 13
Y89
T
D
A
S
F
H
A
Y
D
S
H
T
N
T
Y
Site 14
S91
A
S
F
H
A
Y
D
S
H
T
N
T
Y
Y
L
Site 15
T95
A
Y
D
S
H
T
N
T
Y
Y
L
Q
T
F
G
Site 16
Y96
Y
D
S
H
T
N
T
Y
Y
L
Q
T
F
G
H
Site 17
Y97
D
S
H
T
N
T
Y
Y
L
Q
T
F
G
H
N
Site 18
T100
T
N
T
Y
Y
L
Q
T
F
G
H
N
T
M
D
Site 19
T105
L
Q
T
F
G
H
N
T
M
D
A
V
P
K
I
Site 20
Y114
D
A
V
P
K
I
E
Y
Y
R
N
T
G
S
I
Site 21
Y115
A
V
P
K
I
E
Y
Y
R
N
T
G
S
I
S
Site 22
T118
K
I
E
Y
Y
R
N
T
G
S
I
S
G
P
K
Site 23
S120
E
Y
Y
R
N
T
G
S
I
S
G
P
K
V
N
Site 24
S122
Y
R
N
T
G
S
I
S
G
P
K
V
N
R
P
Site 25
S130
G
P
K
V
N
R
P
S
L
L
E
I
H
E
Q
Site 26
S148
N
V
A
V
T
P
S
S
A
D
R
V
A
N
G
Site 27
S249
G
G
A
Y
Y
L
I
S
R
S
L
G
P
E
F
Site 28
S251
A
Y
Y
L
I
S
R
S
L
G
P
E
F
G
G
Site 29
S292
D
L
L
K
E
S
D
S
M
M
V
D
P
T
N
Site 30
T298
D
S
M
M
V
D
P
T
N
D
I
R
I
I
G
Site 31
S355
P
S
N
N
E
K
K
S
R
G
F
F
N
Y
Q
Site 32
Y361
K
S
R
G
F
F
N
Y
Q
A
S
I
F
A
E
Site 33
T375
E
N
F
G
P
R
F
T
K
G
E
G
F
F
S
Site 34
T448
A
T
G
N
M
N
D
T
I
I
S
G
M
N
C
Site 35
Y465
S
A
A
C
G
L
G
Y
D
F
S
R
C
R
H
Site 36
Y591
F
S
C
F
H
A
S
Y
A
K
S
P
G
W
R
Site 37
S594
F
H
A
S
Y
A
K
S
P
G
W
R
P
A
Y
Site 38
S653
K
P
D
V
N
W
G
S
S
T
Q
A
L
S
Y
Site 39
S654
P
D
V
N
W
G
S
S
T
Q
A
L
S
Y
V
Site 40
T655
D
V
N
W
G
S
S
T
Q
A
L
S
Y
V
S
Site 41
S659
G
S
S
T
Q
A
L
S
Y
V
S
A
L
D
N
Site 42
Y660
S
S
T
Q
A
L
S
Y
V
S
A
L
D
N
A
Site 43
T688
R
P
Q
C
I
V
L
T
G
G
P
M
T
R
P
Site 44
T701
R
P
A
L
L
D
I
T
H
A
F
T
K
N
S
Site 45
T776
L
G
R
M
K
P
N
T
L
V
I
G
Y
K
K
Site 46
Y781
P
N
T
L
V
I
G
Y
K
K
N
W
R
K
A
Site 47
T791
N
W
R
K
A
P
L
T
E
I
E
N
Y
V
G
Site 48
S822
I
S
Q
G
F
D
I
S
Q
V
L
Q
V
Q
E
Site 49
T872
G
K
L
N
I
T
K
T
T
P
K
K
D
G
S
Site 50
T873
K
L
N
I
T
K
T
T
P
K
K
D
G
S
I
Site 51
S879
T
T
P
K
K
D
G
S
I
N
T
S
Q
S
M
Site 52
S883
K
D
G
S
I
N
T
S
Q
S
M
H
V
G
E
Site 53
S885
G
S
I
N
T
S
Q
S
M
H
V
G
E
F
N
Site 54
T931
L
L
I
P
Y
I
L
T
L
R
K
K
W
K
D
Site 55
Y944
K
D
C
K
L
R
I
Y
V
G
G
K
I
N
R
Site 56
S1003
E
P
Y
R
L
H
E
S
C
K
D
L
T
T
A
Site 57
T1008
H
E
S
C
K
D
L
T
T
A
E
K
L
K
R
Site 58
T1017
A
E
K
L
K
R
E
T
P
W
K
I
T
D
A
Site 59
S1033
L
E
A
V
K
E
K
S
Y
R
Q
V
R
L
N
Site 60
S1047
N
E
L
L
Q
E
H
S
R
A
A
N
L
I
V
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation