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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
MELK
Full Name:
Maternal embryonic leucine zipper kinase
Alias:
EC 2.7.11.1; KIAA0175; PEg3 kinase; PK38; Protein kinase PK38
Type:
Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1;
CAMK
group; CAMKL family; MELK subfamily
Mass (Da):
74642
Number AA:
651
UniProt ID:
Q14680
International Prot ID:
IPI00006471
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
GO:0005737
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0005515
GO:0004674
PhosphoSite+
KinaseNET
Biological Process:
GO:0006468
Phosida
TranscriptoNet
STRING
Kinexus Products
Maternal embryonic leucine zipper kinase pan-specific antibody AB-NK229-1#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK229-1#Maternal embryonic leucine zipper kinase pan-specific antibody AB-NK229-2#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK229-2#Maternal embryonic leucine zipper kinase pan-specific antibody AB-NK229-2P#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK229-2P#Maternal embryonic leucine zipper kinase pan-specific antibody AB-NK229-3#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-NK229-3#Maternal embryonic leucine zipper kinase Y438 phosphosite-specific antibody AB-PK701#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK701#Maternal embryonic leucine zipper kinase (G159-G170, human) pY163+pT167 phosphopeptide - Powder PE-04AWJ90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AWJ90
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y4
_
_
_
_
M
K
D
Y
D
E
L
L
K
Y
Y
Site 2
Y10
D
Y
D
E
L
L
K
Y
Y
E
L
H
E
T
I
Site 3
Y11
Y
D
E
L
L
K
Y
Y
E
L
H
E
T
I
G
Site 4
T16
K
Y
Y
E
L
H
E
T
I
G
T
G
G
F
A
Site 5
T46
I
K
I
M
D
K
N
T
L
G
S
D
L
P
R
Site 6
S49
M
D
K
N
T
L
G
S
D
L
P
R
I
K
T
Site 7
T56
S
D
L
P
R
I
K
T
E
I
E
A
L
K
N
Site 8
Y73
H
Q
H
I
C
Q
L
Y
H
V
L
E
T
A
N
Site 9
Y88
K
I
F
M
V
L
E
Y
C
P
G
G
E
L
F
Site 10
Y97
P
G
G
E
L
F
D
Y
I
I
S
Q
D
R
L
Site 11
S100
E
L
F
D
Y
I
I
S
Q
D
R
L
S
E
E
Site 12
S105
I
I
S
Q
D
R
L
S
E
E
E
T
R
V
V
Site 13
T109
D
R
L
S
E
E
E
T
R
V
V
F
R
Q
I
Site 14
S125
S
A
V
A
Y
V
H
S
Q
G
Y
A
H
R
D
Site 15
Y143
E
N
L
L
F
D
E
Y
H
K
L
K
L
I
D
Site 16
Y163
K
P
K
G
N
K
D
Y
H
L
Q
T
C
C
G
Maternal embryonic leucine zipper kinase (G159-G170, human) pY163+pT167 phosphopeptide - Powder PE-04AWJ90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AWJ90
Site 17
T167
N
K
D
Y
H
L
Q
T
C
C
G
S
L
A
Y
Maternal embryonic leucine zipper kinase (G159-G170, human) pY163+pT167 phosphopeptide - Powder PE-04AWJ90#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AWJ90
Site 18
S171
H
L
Q
T
C
C
G
S
L
A
Y
A
A
P
E
Site 19
S184
P
E
L
I
Q
G
K
S
Y
L
G
S
E
A
D
Site 20
S188
Q
G
K
S
Y
L
G
S
E
A
D
V
W
S
M
Site 21
Y218
D
D
N
V
M
A
L
Y
K
K
I
M
R
G
K
Site 22
Y226
K
K
I
M
R
G
K
Y
D
V
P
K
W
L
S
Site 23
S253
V
D
P
K
K
R
I
S
M
K
N
L
L
N
H
Site 24
Y267
H
P
W
I
M
Q
D
Y
N
Y
P
V
E
W
Q
Site 25
Y269
W
I
M
Q
D
Y
N
Y
P
V
E
W
Q
S
K
Site 26
T288
H
L
D
D
D
C
V
T
E
L
S
V
H
H
R
Site 27
T300
H
H
R
N
N
R
Q
T
M
E
D
L
I
S
L
Site 28
Y310
D
L
I
S
L
W
Q
Y
D
H
L
T
A
T
Y
Site 29
S335
K
P
V
R
L
R
L
S
S
F
S
C
G
Q
A
Site 30
S336
P
V
R
L
R
L
S
S
F
S
C
G
Q
A
S
Site 31
S338
R
L
R
L
S
S
F
S
C
G
Q
A
S
A
T
Site 32
S343
S
F
S
C
G
Q
A
S
A
T
P
F
T
D
I
Site 33
T345
S
C
G
Q
A
S
A
T
P
F
T
D
I
K
S
Site 34
T348
Q
A
S
A
T
P
F
T
D
I
K
S
N
N
W
Site 35
S352
T
P
F
T
D
I
K
S
N
N
W
S
L
E
D
Site 36
S356
D
I
K
S
N
N
W
S
L
E
D
V
T
A
S
Site 37
T361
N
W
S
L
E
D
V
T
A
S
D
K
N
Y
V
Site 38
S363
S
L
E
D
V
T
A
S
D
K
N
Y
V
A
G
Site 39
Y367
V
T
A
S
D
K
N
Y
V
A
G
L
I
D
Y
Site 40
Y374
Y
V
A
G
L
I
D
Y
D
W
C
E
D
D
L
Site 41
S382
D
W
C
E
D
D
L
S
T
G
A
A
T
P
R
Site 42
T387
D
L
S
T
G
A
A
T
P
R
T
S
Q
F
T
Site 43
T390
T
G
A
A
T
P
R
T
S
Q
F
T
K
Y
W
Site 44
S391
G
A
A
T
P
R
T
S
Q
F
T
K
Y
W
T
Site 45
T394
T
P
R
T
S
Q
F
T
K
Y
W
T
E
S
N
Site 46
Y396
R
T
S
Q
F
T
K
Y
W
T
E
S
N
G
V
Site 47
T398
S
Q
F
T
K
Y
W
T
E
S
N
G
V
E
S
Site 48
S400
F
T
K
Y
W
T
E
S
N
G
V
E
S
K
S
Site 49
S405
T
E
S
N
G
V
E
S
K
S
L
T
P
A
L
Site 50
S407
S
N
G
V
E
S
K
S
L
T
P
A
L
C
R
Site 51
T409
G
V
E
S
K
S
L
T
P
A
L
C
R
T
P
Site 52
T415
L
T
P
A
L
C
R
T
P
A
N
K
L
K
N
Site 53
Y427
L
K
N
K
E
N
V
Y
T
P
K
S
A
V
K
Site 54
T428
K
N
K
E
N
V
Y
T
P
K
S
A
V
K
N
Site 55
S431
E
N
V
Y
T
P
K
S
A
V
K
N
E
E
Y
Site 56
Y438
S
A
V
K
N
E
E
Y
F
M
F
P
E
P
K
Maternal embryonic leucine zipper kinase Y438 phosphosite-specific antibody AB-PK701#http://www.kinexusproducts.ca/ProductInfo_Antibody.aspx?Product_Number=AB-PK701
Site 57
T446
F
M
F
P
E
P
K
T
P
V
N
K
N
Q
H
Site 58
T459
Q
H
K
R
E
I
L
T
T
P
N
R
Y
T
T
Site 59
T460
H
K
R
E
I
L
T
T
P
N
R
Y
T
T
P
Site 60
Y464
I
L
T
T
P
N
R
Y
T
T
P
S
K
A
R
Site 61
T465
L
T
T
P
N
R
Y
T
T
P
S
K
A
R
N
Site 62
T466
T
T
P
N
R
Y
T
T
P
S
K
A
R
N
Q
Site 63
S468
P
N
R
Y
T
T
P
S
K
A
R
N
Q
C
L
Site 64
T478
R
N
Q
C
L
K
E
T
P
I
K
I
P
V
N
Site 65
S486
P
I
K
I
P
V
N
S
T
G
T
D
K
L
M
Site 66
T494
T
G
T
D
K
L
M
T
G
V
I
S
P
E
R
Site 67
S498
K
L
M
T
G
V
I
S
P
E
R
R
C
R
S
Site 68
S505
S
P
E
R
R
C
R
S
V
E
L
D
L
N
Q
Site 69
T518
N
Q
A
H
M
E
E
T
P
K
R
K
G
A
K
Site 70
S529
K
G
A
K
V
F
G
S
L
E
R
G
L
D
K
Site 71
T539
R
G
L
D
K
V
I
T
V
L
T
R
S
K
R
Site 72
S544
V
I
T
V
L
T
R
S
K
R
K
G
S
A
R
Site 73
S549
T
R
S
K
R
K
G
S
A
R
D
G
P
R
R
Site 74
Y561
P
R
R
L
K
L
H
Y
N
V
T
T
T
R
L
Site 75
T564
L
K
L
H
Y
N
V
T
T
T
R
L
V
N
P
Site 76
T600
G
Y
T
L
K
C
Q
T
Q
S
D
F
G
K
V
Site 77
Y638
L
K
G
D
A
W
V
Y
K
R
L
V
E
D
I
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation