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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated November 2019
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Protein Info
Short Name:
Hpk1
Full Name:
Mitogen-activated protein kinase kinase kinase kinase 1
Alias:
EC 2.7.11.1; Hematopoietic progenitor kinase; Hematopoietic progenitor kinase 1; HPK1; Kinase HPK1; M4K1; MAP4K1
Type:
EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor);
STE
group; STE20 family; KHS subfamily
Mass (Da):
91296
Number AA:
833
UniProt ID:
Q92918
International Prot ID:
IPI00020258
Sequence:
Retrieve full protein sequence
GO Terms
External Links
Internal Links
Cellular Component:
Uniprot
OncoNet
Molecular Function:
GO:0005524
GO:0008349
GO:0005515
PhosphoSite+
KinaseNET
Biological Process:
GO:0007257
GO:0000185
GO:0018105
Phosida
TranscriptoNet
STRING
Kinexus Products
Mitogen-activated protein kinase kinase kinase kinase 1 (I162-G174, human) pT165+pS171 phosphopeptide - Powder PE-04AUV99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AUV99
Info Box
A variety of parameters were considered in the selection of putative P-sites. Confirmed P-Sites have lower Hydrophobicity Scores. The P-site Similarity Score is lower the more that it resembles typical confirmed corresponding P-Ser, P-Thr or P-Tyr sites. The Maximum KInase Score provides the calculated score for highest match of 500 human protein kinases for the amino acid sequence surrounding the target P-site as determined with Kinase Substrate Predictor V2. The Sum KInase Score provides the additive sum of the positive individual Kinase Substrate Predictor V2 scores from 500 human protein kinases. The Conservation Score is the average of the percent similarity of the human P-site with the equivalent P-site in 20 other diverse species. Click the coloured buttons below to retrieve this and other information in the Info Box, or click the orange buttons for relevant links.
Phosphosites
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Expt. conf.
Effect
Kinase
PPase
Kinexus Products
Ref.
Evol.
Kinase Pred.
P-site Match
Site 1
Y17
N
R
D
P
R
D
H
Y
D
L
L
Q
R
L
G
Site 2
Y28
Q
R
L
G
G
G
T
Y
G
E
V
F
K
A
R
Site 3
S57
M
E
P
D
D
D
V
S
T
L
Q
K
E
I
L
Site 4
T58
E
P
D
D
D
V
S
T
L
Q
K
E
I
L
I
Site 5
Y77
R
H
A
N
I
V
A
Y
H
G
S
Y
L
W
L
Site 6
Y103
A
G
S
L
Q
D
I
Y
Q
V
T
G
S
L
S
Site 7
S108
D
I
Y
Q
V
T
G
S
L
S
E
L
Q
I
S
Site 8
Y127
E
V
L
Q
G
L
A
Y
L
H
S
Q
K
K
I
Site 9
S130
Q
G
L
A
Y
L
H
S
Q
K
K
I
H
R
D
Site 10
T165
I
S
A
Q
I
G
A
T
L
A
R
R
L
S
F
Mitogen-activated protein kinase kinase kinase kinase 1 (I162-G174, human) pT165+pS171 phosphopeptide - Powder PE-04AUV99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AUV99
Site 11
S171
A
T
L
A
R
R
L
S
F
I
G
T
P
Y
W
Mitogen-activated protein kinase kinase kinase kinase 1 (I162-G174, human) pT165+pS171 phosphopeptide - Powder PE-04AUV99#http://www.kinexusproducts.ca/ProductInfo_Peptide.aspx?Product_Number=PE-04AUV99
Site 12
T175
R
R
L
S
F
I
G
T
P
Y
W
M
A
P
E
Site 13
Y232
F
L
M
T
K
S
G
Y
Q
P
P
R
L
K
E
Site 14
T254
F
H
N
F
I
K
V
T
L
T
K
S
P
K
K
Site 15
T256
N
F
I
K
V
T
L
T
K
S
P
K
K
R
P
Site 16
S258
I
K
V
T
L
T
K
S
P
K
K
R
P
S
A
Site 17
S264
K
S
P
K
K
R
P
S
A
T
K
M
L
S
H
Site 18
T266
P
K
K
R
P
S
A
T
K
M
L
S
H
Q
L
Site 19
S270
P
S
A
T
K
M
L
S
H
Q
L
V
S
Q
P
Site 20
S275
M
L
S
H
Q
L
V
S
Q
P
G
L
N
R
G
Site 21
S320
A
I
P
R
R
I
R
S
T
H
R
S
S
S
L
Site 22
T321
I
P
R
R
I
R
S
T
H
R
S
S
S
L
G
Site 23
S324
R
I
R
S
T
H
R
S
S
S
L
G
I
P
D
Site 24
S325
I
R
S
T
H
R
S
S
S
L
G
I
P
D
A
Site 25
S326
R
S
T
H
R
S
S
S
L
G
I
P
D
A
D
Site 26
T349
R
K
L
R
G
M
E
T
R
P
P
A
N
T
A
Site 27
T355
E
T
R
P
P
A
N
T
A
R
L
Q
P
P
R
Site 28
S366
Q
P
P
R
D
L
R
S
S
S
P
R
K
Q
L
Site 29
S367
P
P
R
D
L
R
S
S
S
P
R
K
Q
L
S
Site 30
S368
P
R
D
L
R
S
S
S
P
R
K
Q
L
S
E
Site 31
S374
S
S
P
R
K
Q
L
S
E
S
S
D
D
D
Y
Site 32
S376
P
R
K
Q
L
S
E
S
S
D
D
D
Y
D
D
Site 33
S377
R
K
Q
L
S
E
S
S
D
D
D
Y
D
D
V
Site 34
Y381
S
E
S
S
D
D
D
Y
D
D
V
D
I
P
T
Site 35
T388
Y
D
D
V
D
I
P
T
P
A
E
D
T
P
P
Site 36
T393
I
P
T
P
A
E
D
T
P
P
P
L
P
P
K
Site 37
S405
P
P
K
P
K
F
R
S
P
S
D
E
G
P
G
Site 38
S407
K
P
K
F
R
S
P
S
D
E
G
P
G
S
M
Site 39
S413
P
S
D
E
G
P
G
S
M
G
D
D
G
Q
L
Site 40
S421
M
G
D
D
G
Q
L
S
P
G
V
L
V
R
C
Site 41
S430
G
V
L
V
R
C
A
S
G
P
P
P
N
S
P
Site 42
S436
A
S
G
P
P
P
N
S
P
R
P
G
P
P
P
Site 43
S444
P
R
P
G
P
P
P
S
T
S
S
P
H
L
T
Site 44
T445
R
P
G
P
P
P
S
T
S
S
P
H
L
T
A
Site 45
S446
P
G
P
P
P
S
T
S
S
P
H
L
T
A
H
Site 46
S447
G
P
P
P
S
T
S
S
P
H
L
T
A
H
S
Site 47
T451
S
T
S
S
P
H
L
T
A
H
S
E
P
S
L
Site 48
S454
S
P
H
L
T
A
H
S
E
P
S
L
W
N
P
Site 49
S457
L
T
A
H
S
E
P
S
L
W
N
P
P
S
R
Site 50
S463
P
S
L
W
N
P
P
S
R
E
L
D
K
P
P
Site 51
Y546
P
S
R
T
T
W
V
Y
S
I
N
N
V
L
M
Site 52
S554
S
I
N
N
V
L
M
S
L
S
G
K
T
P
H
Site 53
S556
N
N
V
L
M
S
L
S
G
K
T
P
H
L
Y
Site 54
T559
L
M
S
L
S
G
K
T
P
H
L
Y
S
H
S
Site 55
Y563
S
G
K
T
P
H
L
Y
S
H
S
I
L
G
L
Site 56
S566
T
P
H
L
Y
S
H
S
I
L
G
L
L
E
R
Site 57
T576
G
L
L
E
R
K
E
T
R
A
G
N
P
I
A
Site 58
S586
G
N
P
I
A
H
I
S
P
H
R
L
L
A
R
Site 59
S598
L
A
R
K
N
M
V
S
T
K
I
Q
D
T
K
Site 60
T599
A
R
K
N
M
V
S
T
K
I
Q
D
T
K
G
Site 61
Y637
S
V
V
L
L
Q
W
Y
Q
P
M
N
K
F
L
Site 62
S678
P
A
V
C
I
G
V
S
P
G
R
P
G
K
S
Site 63
S685
S
P
G
R
P
G
K
S
V
L
F
H
T
V
R
Site 64
T733
D
G
S
V
K
L
V
T
P
E
G
S
P
V
R
Site 65
S737
K
L
V
T
P
E
G
S
P
V
R
G
L
R
T
Site 66
T744
S
P
V
R
G
L
R
T
P
E
I
P
M
T
E
Site 67
T789
L
Q
E
L
R
D
P
T
L
T
F
R
L
L
G
Site 68
T791
E
L
R
D
P
T
L
T
F
R
L
L
G
S
P
Site 69
S803
G
S
P
R
L
E
C
S
G
T
I
S
P
H
C
Site 70
T805
P
R
L
E
C
S
G
T
I
S
P
H
C
N
L
Site 71
S817
C
N
L
L
L
P
G
S
S
N
S
P
A
S
A
Site 72
S818
N
L
L
L
P
G
S
S
N
S
P
A
S
A
S
Site 73
S820
L
L
P
G
S
S
N
S
P
A
S
A
S
R
V
Site 74
S823
G
S
S
N
S
P
A
S
A
S
R
V
A
G
I
Site 75
S825
S
N
S
P
A
S
A
S
R
V
A
G
I
T
G
Legend
Confirmed in mammals
Confirmed in related proteins or other species
Predicted by Kinexus P-Site Prediction algorithm
No data/link available
Link available
Products available
2019 Kinexus Bioinformatics Corporation