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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ENO4 All Species: 5.76
Human Site: S335 Identified Species: 12.67
UniProt: A6NNW6 Number Species: 10
    Phosphosite Substitution
    Charge Score: -0.1
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens A6NNW6 XP_944914 628 68821 S335 I I E M M P P S P P K A E T K
Chimpanzee Pan troglodytes XP_001145271 552 60831 T280 T L V I Q Q I T G K M S H L G
Rhesus Macaque Macaca mulatta XP_001095040 630 68852 S337 A W R C T L P S P P K A E T K
Dog Lupus familis XP_535025 487 52789 T215 K R G Q Q P I T G K M S H L G
Cat Felis silvestris
Mouse Mus musculus Q8C042 619 67864 P335 N R A M E T L P P P K Q E T K
Rat Rattus norvegicus NP_001127977 602 66248 P330 N R A M E T L P P P K P E T K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512090 642 70002 P352 K L L D P V P P H K T E N K K
Chicken Gallus gallus XP_421780 320 35609 Y48 L Y G E L A N Y F S K F S R A
Frog Xenopus laevis A9UMP7 572 62400 Q300 P P T G L T V Q Q S L D M A L
Zebra Danio Brachydanio rerio Q08BC6 576 62749 I304 L N L L E E I I L M P S S S L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_788156 580 64420 K308 L P L E E G M K Q V T R V Y H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 81.5 93.1 60.3 N.A. 72.4 71.6 N.A. 58.7 25.6 48.5 37.9 N.A. N.A. N.A. N.A. 33.4
Protein Similarity: 100 82.3 95.2 67 N.A. 82.8 81.6 N.A. 73.6 38.6 63.6 54.9 N.A. N.A. N.A. N.A. 50.9
P-Site Identity: 100 0 60 6.6 N.A. 46.6 46.6 N.A. 13.3 6.6 0 0 N.A. N.A. N.A. N.A. 0
P-Site Similarity: 100 26.6 60 20 N.A. 46.6 46.6 N.A. 20 20 6.6 26.6 N.A. N.A. N.A. N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 10 0 19 0 0 10 0 0 0 0 0 19 0 10 10 % A
% Cys: 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 10 0 0 0 0 0 0 0 10 0 0 0 % D
% Glu: 0 0 10 19 37 10 0 0 0 0 0 10 37 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 10 0 0 10 0 0 0 % F
% Gly: 0 0 19 10 0 10 0 0 19 0 0 0 0 0 19 % G
% His: 0 0 0 0 0 0 0 0 10 0 0 0 19 0 10 % H
% Ile: 10 10 0 10 0 0 28 10 0 0 0 0 0 0 0 % I
% Lys: 19 0 0 0 0 0 0 10 0 28 46 0 0 10 46 % K
% Leu: 28 19 28 10 19 10 19 0 10 0 10 0 0 19 19 % L
% Met: 0 0 0 28 10 0 10 0 0 10 19 0 10 0 0 % M
% Asn: 19 10 0 0 0 0 10 0 0 0 0 0 10 0 0 % N
% Pro: 10 19 0 0 10 19 28 28 37 37 10 10 0 0 0 % P
% Gln: 0 0 0 10 19 10 0 10 19 0 0 10 0 0 0 % Q
% Arg: 0 28 10 0 0 0 0 0 0 0 0 10 0 10 0 % R
% Ser: 0 0 0 0 0 0 0 19 0 19 0 28 19 10 0 % S
% Thr: 10 0 10 0 10 28 0 19 0 0 19 0 0 37 0 % T
% Val: 0 0 10 0 0 10 10 0 0 10 0 0 10 0 0 % V
% Trp: 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 10 0 0 0 0 0 10 0 0 0 0 0 10 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _