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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: GAPDHS All Species: 22.73
Human Site: T225 Identified Species: 50
UniProt: O14556 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0.5
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O14556 NP_055179.1 408 44501 T225 I V S N A S C T T N C L A P L
Chimpanzee Pan troglodytes XP_512590 408 44488 T225 I V S N A S C T T N C L A P L
Rhesus Macaque Macaca mulatta XP_001095332 409 44578 T226 I V S N A S C T T N C L A P L
Dog Lupus familis XP_533693 404 44075 T221 V V S N A S C T T N C L A P L
Cat Felis silvestris
Mouse Mus musculus Q64467 440 47638 T257 I V S N A S C T T N C L A P L
Rat Rattus norvegicus Q9ESV6 432 46689 T249 V V S N A S C T T N C L A P L
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus P00356 333 35685 E170 H D N F G I V E G L M T T V H
Frog Xenopus laevis P51469 333 35679 E170 N D N F G I V E G L M T T V H
Zebra Danio Brachydanio rerio Q5MJ86 335 36089 E172 H D N F G I E E A L M T T V H
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P07487 332 35351 E169 N D N F E I V E G L M T T V H
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P17330 341 36441 E178 N D N F G I I E G L M T T V H
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 98.7 95.3 84.3 N.A. 76.8 79.1 N.A. N.A. 56.8 56.6 64.2 N.A. 57.5 N.A. 57.5 N.A.
Protein Similarity: 100 99.2 97.3 91.1 N.A. 83.8 86.1 N.A. N.A. 67.1 67.6 71.8 N.A. 67.4 N.A. 68.3 N.A.
P-Site Identity: 100 100 100 93.3 N.A. 100 93.3 N.A. N.A. 0 0 0 N.A. 0 N.A. 0 N.A.
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. N.A. 6.6 6.6 6.6 N.A. 6.6 N.A. 6.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 55 0 0 0 10 0 0 0 55 0 0 % A
% Cys: 0 0 0 0 0 0 55 0 0 0 55 0 0 0 0 % C
% Asp: 0 46 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 10 0 10 46 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 46 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 37 0 0 0 37 0 0 0 0 0 0 % G
% His: 19 0 0 0 0 0 0 0 0 0 0 0 0 0 46 % H
% Ile: 37 0 0 0 0 46 10 0 0 0 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 0 0 0 0 0 0 0 0 0 46 0 55 0 0 55 % L
% Met: 0 0 0 0 0 0 0 0 0 0 46 0 0 0 0 % M
% Asn: 28 0 46 55 0 0 0 0 0 55 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 55 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 0 55 0 0 55 0 0 0 0 0 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 55 55 0 0 46 46 0 0 % T
% Val: 19 55 0 0 0 0 28 0 0 0 0 0 0 46 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _