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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: EIF5B All Species: 21.52
Human Site: T486 Identified Species: 33.81
UniProt: O60841 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O60841 NP_056988.3 1220 138827 T486 G V P E K E E T P P P V E P E
Chimpanzee Pan troglodytes XP_001159716 1220 138677 T486 G V P E K E E T P P P V E P E
Rhesus Macaque Macaca mulatta XP_001103720 1526 171398 T791 G V P E K E E T P P P V E P E
Dog Lupus familis XP_851262 1220 138726 T486 G V P E K E E T P P P V E P E
Cat Felis silvestris
Mouse Mus musculus Q05D44 1216 137597 T485 G G P E K E E T P P S V E P E
Rat Rattus norvegicus B2GUV7 1216 137668 T485 G V P E K E E T P P S V D A E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001507187 1282 144754 E555 E P A V T D Q E E T T P P V E
Chicken Gallus gallus NP_001033585 1220 137737 L481 L V K E E P P L P A E P E E K
Frog Xenopus laevis
Zebra Danio Brachydanio rerio XP_001923573 1166 130209 K453 K D A M P K K K P V Y S D K R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_651974 1144 127185 G418 I R P N K K K G P K E E A A E
Honey Bee Apis mellifera XP_392101 1028 116618 Q364 A E T S E D E Q D E E K Q S Q
Nematode Worm Caenorhab. elegans NP_497536 1074 120399 A356 P V A A E A S A D A S A S A S
Sea Urchin Strong. purpuratus XP_001199006 1349 151384 K606 K E R Q K Q R K K E R I E Q M
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_177807 1294 142098 I534 K S S R Q K G I D T S V Q G E
Baker's Yeast Sacchar. cerevisiae P39730 1002 112250 E359 D Q N Q G E E E E E G E E E E
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.6 79.2 96 N.A. 91.6 90 N.A. 82.2 84.5 N.A. 64.3 N.A. 50.8 53.6 40.3 43.5
Protein Similarity: 100 99.7 79.7 97.8 N.A. 95.1 94.5 N.A. 87.6 90.5 N.A. 75.4 N.A. 66.7 67.7 61.1 62.7
P-Site Identity: 100 100 100 100 N.A. 86.6 80 N.A. 6.6 26.6 N.A. 6.6 N.A. 26.6 6.6 6.6 13.3
P-Site Similarity: 100 100 100 100 N.A. 86.6 86.6 N.A. 20 40 N.A. 26.6 N.A. 40 33.3 13.3 33.3
Percent
Protein Identity: N.A. N.A. N.A. 38.3 39.1 N.A.
Protein Similarity: N.A. N.A. N.A. 57.9 57.5 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 26.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 20 7 0 7 0 7 0 14 0 7 7 20 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 7 0 0 0 14 0 0 20 0 0 0 14 0 0 % D
% Glu: 7 14 0 47 20 47 54 14 14 20 20 14 54 14 67 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 40 7 0 0 7 0 7 7 0 0 7 0 0 7 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 7 0 0 0 0 0 0 7 0 0 0 7 0 0 0 % I
% Lys: 20 0 7 0 54 20 14 14 7 7 0 7 0 7 7 % K
% Leu: 7 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % L
% Met: 0 0 0 7 0 0 0 0 0 0 0 0 0 0 7 % M
% Asn: 0 0 7 7 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 7 7 47 0 7 7 7 0 60 40 27 14 7 34 0 % P
% Gln: 0 7 0 14 7 7 7 7 0 0 0 0 14 7 7 % Q
% Arg: 0 7 7 7 0 0 7 0 0 0 7 0 0 0 7 % R
% Ser: 0 7 7 7 0 0 7 0 0 0 27 7 7 7 7 % S
% Thr: 0 0 7 0 7 0 0 40 0 14 7 0 0 0 0 % T
% Val: 0 47 0 7 0 0 0 0 0 7 0 47 0 7 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 7 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _