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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: DTNB All Species: 14.24
Human Site: T424 Identified Species: 28.48
UniProt: O60941 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0.09
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O60941 NP_068707.1 627 71356 T424 G N V T R P P T D L S F N F D
Chimpanzee Pan troglodytes XP_515336 627 71305 T424 G N V T R P P T D L S F N F D
Rhesus Macaque Macaca mulatta XP_001102769 743 83913 Q456 S S S Q P T Q Q R S A P D I S
Dog Lupus familis XP_540111 670 76530 A424 G N M T R P P A D L S F N F D
Cat Felis silvestris
Mouse Mus musculus O70585 700 78337 T424 G N M T R P P T D A S F N F D
Rat Rattus norvegicus P84060 654 73859 T424 G N M T R P P T D A S F N F D
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001515697 743 83772 Q456 S S S Q P G Q Q R G A S D I S
Chicken Gallus gallus XP_419187 742 83445 Q455 S S S Q Q A Q Q R G A A D I S
Frog Xenopus laevis NP_001079191 743 83797 G453 S S S V Q Q R G G S D I T F T
Zebra Danio Brachydanio rerio A2CI97 648 73379 M423 C S E L K S M M E T L N Q H N
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q9Y048 590 65408 S433 S N G R S M N S S M V G D E R
Sea Urchin Strong. purpuratus XP_797247 727 82017 E406 R E L I M Q L E S K N R E I M
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.8 64 89.5 N.A. 84 88.6 N.A. 64 64.4 64.1 21.4 N.A. N.A. N.A. 42.5 37.9
Protein Similarity: 100 100 74.2 91.7 N.A. 86.1 90.5 N.A. 74 73.8 74.6 40.4 N.A. N.A. N.A. 60.1 55.3
P-Site Identity: 100 100 0 86.6 N.A. 86.6 86.6 N.A. 0 0 6.6 0 N.A. N.A. N.A. 6.6 0
P-Site Similarity: 100 100 20 93.3 N.A. 93.3 93.3 N.A. 20 26.6 20 26.6 N.A. N.A. N.A. 26.6 13.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 9 0 9 0 17 25 9 0 0 0 % A
% Cys: 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 42 0 9 0 34 0 42 % D
% Glu: 0 9 9 0 0 0 0 9 9 0 0 0 9 9 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 42 0 50 0 % F
% Gly: 42 0 9 0 0 9 0 9 9 17 0 9 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 % H
% Ile: 0 0 0 9 0 0 0 0 0 0 0 9 0 34 0 % I
% Lys: 0 0 0 0 9 0 0 0 0 9 0 0 0 0 0 % K
% Leu: 0 0 9 9 0 0 9 0 0 25 9 0 0 0 0 % L
% Met: 0 0 25 0 9 9 9 9 0 9 0 0 0 0 9 % M
% Asn: 0 50 0 0 0 0 9 0 0 0 9 9 42 0 9 % N
% Pro: 0 0 0 0 17 42 42 0 0 0 0 9 0 0 0 % P
% Gln: 0 0 0 25 17 17 25 25 0 0 0 0 9 0 0 % Q
% Arg: 9 0 0 9 42 0 9 0 25 0 0 9 0 0 9 % R
% Ser: 42 42 34 0 9 9 0 9 17 17 42 9 0 0 25 % S
% Thr: 0 0 0 42 0 9 0 34 0 9 0 0 9 0 9 % T
% Val: 0 0 17 9 0 0 0 0 0 0 9 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _