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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: RPS6KA4 All Species: 33.94
Human Site: S423 Identified Species: 49.78
UniProt: O75676 Number Species: 15
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O75676 NP_001006945.1 772 85606 S423 A L G Q G S F S V C R R C R Q
Chimpanzee Pan troglodytes XP_508900 901 98668 S552 A L G Q G S F S V C R R C R Q
Rhesus Macaque Macaca mulatta XP_001114877 772 85557 S423 A L G Q G S F S V C R R C R Q
Dog Lupus familis XP_854926 772 85603 S424 A L G Q G S F S V C R R C R Q
Cat Felis silvestris
Mouse Mus musculus Q9Z2B9 773 85721 S423 A L G Q G S F S V C R R C R Q
Rat Rattus norvegicus Q63531 735 82865 A398 S K P R A T Q A P L H S V V Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506738 716 80894 C360 S F S I C R K C L H K K S N Q
Chicken Gallus gallus Q5F3L1 789 89022 S428 P L G E G S F S I C R K C L H
Frog Xenopus laevis P10665 733 82620 S401 T S S P P V L S V P K T H S K
Zebra Danio Brachydanio rerio Q6PFQ0 740 83378 P405 A P L V N I L P I V Q V H G S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9V3I5 1207 137026 C637 S N G A Y G T C H F V V D S S
Honey Bee Apis mellifera XP_395099 910 102507 S463 A L G D G S F S V C R K C R H
Nematode Worm Caenorhab. elegans Q18846 772 87050 S394 L L G K G A F S V V R R C E R
Sea Urchin Strong. purpuratus XP_786494 918 102561 S397 P I G D G S F S I C R R C T H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q39030 471 53019 E141 V V G I E D F E V L K V V G Q
Baker's Yeast Sacchar. cerevisiae P18961 677 76646 V349 D D F D L L K V I G K G S F G
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 85.4 98.6 96.7 N.A. 95.8 41.3 N.A. 56 64 41 41.9 N.A. 27 45.1 48.1 48.2
Protein Similarity: 100 85.6 98.9 98 N.A. 96.7 60.6 N.A. 71.6 78.4 59 60.3 N.A. 38.9 60.1 64.3 61.8
P-Site Identity: 100 100 100 100 N.A. 100 6.6 N.A. 6.6 60 13.3 6.6 N.A. 6.6 80 60 60
P-Site Similarity: 100 100 100 100 N.A. 100 33.3 N.A. 33.3 80 26.6 20 N.A. 13.3 86.6 80 73.3
Percent
Protein Identity: N.A. N.A. N.A. 20.4 21.2 N.A.
Protein Similarity: N.A. N.A. N.A. 35.6 37.5 N.A.
P-Site Identity: N.A. N.A. N.A. 26.6 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 40 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 44 0 0 7 7 7 0 7 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 7 0 0 13 0 50 0 0 57 0 0 % C
% Asp: 7 7 0 19 0 7 0 0 0 0 0 0 7 0 0 % D
% Glu: 0 0 0 7 7 0 0 7 0 0 0 0 0 7 0 % E
% Phe: 0 7 7 0 0 0 63 0 0 7 0 0 0 7 0 % F
% Gly: 0 0 69 0 57 7 0 0 0 7 0 7 0 13 7 % G
% His: 0 0 0 0 0 0 0 0 7 7 7 0 13 0 19 % H
% Ile: 0 7 0 13 0 7 0 0 25 0 0 0 0 0 0 % I
% Lys: 0 7 0 7 0 0 13 0 0 0 25 19 0 0 7 % K
% Leu: 7 50 7 0 7 7 13 0 7 13 0 0 0 7 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 7 0 0 7 0 0 0 0 0 0 0 0 7 0 % N
% Pro: 13 7 7 7 7 0 0 7 7 7 0 0 0 0 0 % P
% Gln: 0 0 0 32 0 0 7 0 0 0 7 0 0 0 50 % Q
% Arg: 0 0 0 7 0 7 0 0 0 0 57 44 0 38 7 % R
% Ser: 19 7 13 0 0 50 0 63 0 0 0 7 13 13 13 % S
% Thr: 7 0 0 0 0 7 7 0 0 0 0 7 0 7 0 % T
% Val: 7 7 0 7 0 7 0 7 57 13 7 19 13 7 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 7 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _