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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: DGKI All Species: 26.06
Human Site: S333 Identified Species: 47.78
UniProt: O75912 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O75912 NP_004708.1 1065 116997 S333 K V K K P Q N S L K A S N R K
Chimpanzee Pan troglodytes XP_001147695 1066 117064 S334 K V K K P Q N S L K A S N R K
Rhesus Macaque Macaca mulatta XP_001107030 1066 117055 S334 K V K K P Q N S L K A S N R K
Dog Lupus familis XP_539825 1017 113427 S293 K V K K P Q N S L K A S N R K
Cat Felis silvestris
Mouse Mus musculus Q80UP3 929 104029 S266 S K K K K R A S F K R R S S K
Rat Rattus norvegicus O08560 929 103991 S266 S K K K K R A S F K R R S S K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513139 955 107141 F274 G T K V L Q M F M W F L N P R
Chicken Gallus gallus XP_416357 1074 118933 S327 K V K K P Q N S L K T S T R R
Frog Xenopus laevis
Zebra Danio Brachydanio rerio XP_001922445 1086 121319 S322 R V R R P Q S S L K S S K K K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q09103 1457 160124 F748 K L P T K G N F K S S I R V S
Honey Bee Apis mellifera XP_396522 837 92900 K174 Q G K L W V V K P I P T P T V
Nematode Worm Caenorhab. elegans Q10024 937 105180 K273 N C F S L L A K L N L N C K T
Sea Urchin Strong. purpuratus XP_796061 815 92455 V152 K C S S C K I V V H T A C M Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.7 99.4 86.4 N.A. 55.5 56.6 N.A. 75.4 80.7 N.A. 56.6 N.A. 31.6 34.1 34 43.3
Protein Similarity: 100 99.9 99.6 89.1 N.A. 69.3 69.3 N.A. 80.9 86.4 N.A. 69.1 N.A. 44.5 49.4 49.6 55.9
P-Site Identity: 100 100 100 100 N.A. 33.3 33.3 N.A. 20 80 N.A. 53.3 N.A. 13.3 6.6 6.6 6.6
P-Site Similarity: 100 100 100 100 N.A. 46.6 46.6 N.A. 33.3 86.6 N.A. 93.3 N.A. 26.6 20 20 33.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 24 0 0 0 31 8 0 0 0 % A
% Cys: 0 16 0 0 8 0 0 0 0 0 0 0 16 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 8 0 0 0 0 16 16 0 8 0 0 0 0 % F
% Gly: 8 8 0 0 0 8 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 8 0 0 8 0 8 0 0 0 % I
% Lys: 54 16 70 54 24 8 0 16 8 62 0 0 8 16 54 % K
% Leu: 0 8 0 8 16 8 0 0 54 0 8 8 0 0 0 % L
% Met: 0 0 0 0 0 0 8 0 8 0 0 0 0 8 0 % M
% Asn: 8 0 0 0 0 0 47 0 0 8 0 8 39 0 0 % N
% Pro: 0 0 8 0 47 0 0 0 8 0 8 0 8 8 0 % P
% Gln: 8 0 0 0 0 54 0 0 0 0 0 0 0 0 8 % Q
% Arg: 8 0 8 8 0 16 0 0 0 0 16 16 8 39 16 % R
% Ser: 16 0 8 16 0 0 8 62 0 8 16 47 16 16 8 % S
% Thr: 0 8 0 8 0 0 0 0 0 0 16 8 8 8 8 % T
% Val: 0 47 0 8 0 8 8 8 8 0 0 0 0 8 8 % V
% Trp: 0 0 0 0 8 0 0 0 0 8 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _