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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PDE5A All Species: 15.45
Human Site: T399 Identified Species: 26.15
UniProt: O76074 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.23
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O76074 NP_001074.2 875 100013 T399 E K S S D T L T R E H D A N K
Chimpanzee Pan troglodytes XP_001150967 875 99910 T399 E K S S D T L T R E H D A N K
Rhesus Macaque Macaca mulatta XP_001099652 875 100002 T399 E K S S D M L T R E H D T N K
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus Q8CG03 865 98403 T389 G K P S D P L T R E Q D A N K
Rat Rattus norvegicus O54735 833 94538 P379 Y V K N T M E P L N I P D V T
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512580 863 98139 K388 E D S A D A L K R D Y D T N K
Chicken Gallus gallus P52731 862 99990 F370 N A P A D E Y F T F Q K G P V
Frog Xenopus laevis NP_001088271 859 97399 K380 Q E S A N A V K R E Y D V S K
Zebra Danio Brachydanio rerio XP_001923466 856 97075 N384 A G S T D F P N R E C D V N K
Tiger Blowfish Takifugu rubipres Q1KKS3 903 102001 S409 K C S A D A E S S F K D S M E
Fruit Fly Dros. melanogaster Q9VJ79 1451 160911 R657 S E E E A T S R T S P Y E S R
Honey Bee Apis mellifera XP_394107 1016 115998 P436 S M Q E S R K P L S R R E A T
Nematode Worm Caenorhab. elegans P91119 710 81117 A305 K G I A G Y V A S T G E G L N
Sea Urchin Strong. purpuratus NP_001029121 949 108476 S386 H S I A Y Q S S F D A P L N D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.6 99 N.A. N.A. 92.4 88.9 N.A. 87.8 25.1 75.1 71.1 38.1 31.2 37.5 26.6 39
Protein Similarity: 100 99.7 99.4 N.A. N.A. 94.5 91 N.A. 91.6 44.2 85 83.1 58.1 41.3 54.6 44.2 55.9
P-Site Identity: 100 100 86.6 N.A. N.A. 73.3 0 N.A. 53.3 6.6 33.3 46.6 20 6.6 0 0 6.6
P-Site Similarity: 100 100 86.6 N.A. N.A. 73.3 6.6 N.A. 73.3 13.3 80 53.3 53.3 26.6 0 26.6 26.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 8 0 43 8 22 0 8 0 0 8 0 22 8 0 % A
% Cys: 0 8 0 0 0 0 0 0 0 0 8 0 0 0 0 % C
% Asp: 0 8 0 0 58 0 0 0 0 15 0 58 8 0 8 % D
% Glu: 29 15 8 15 0 8 15 0 0 43 0 8 15 0 8 % E
% Phe: 0 0 0 0 0 8 0 8 8 15 0 0 0 0 0 % F
% Gly: 8 15 0 0 8 0 0 0 0 0 8 0 15 0 0 % G
% His: 8 0 0 0 0 0 0 0 0 0 22 0 0 0 0 % H
% Ile: 0 0 15 0 0 0 0 0 0 0 8 0 0 0 0 % I
% Lys: 15 29 8 0 0 0 8 15 0 0 8 8 0 0 50 % K
% Leu: 0 0 0 0 0 0 36 0 15 0 0 0 8 8 0 % L
% Met: 0 8 0 0 0 15 0 0 0 0 0 0 0 8 0 % M
% Asn: 8 0 0 8 8 0 0 8 0 8 0 0 0 50 8 % N
% Pro: 0 0 15 0 0 8 8 15 0 0 8 15 0 8 0 % P
% Gln: 8 0 8 0 0 8 0 0 0 0 15 0 0 0 0 % Q
% Arg: 0 0 0 0 0 8 0 8 50 0 8 8 0 0 8 % R
% Ser: 15 8 50 29 8 0 15 15 15 15 0 0 8 15 0 % S
% Thr: 0 0 0 8 8 22 0 29 15 8 0 0 15 0 15 % T
% Val: 0 8 0 0 0 0 15 0 0 0 0 0 15 8 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 0 0 0 8 8 8 0 0 0 15 8 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _