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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP3B All Species: 15.76
Human Site: S25 Identified Species: 28.89
UniProt: O95985 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens O95985 NP_003926.1 862 96662 S25 A K I L S R G S L S S H K G L
Chimpanzee Pan troglodytes XP_515006 956 106787 S119 A K I L S R G S L S S H K G L
Rhesus Macaque Macaca mulatta XP_001089027 862 96675 S25 A K I L S R G S L S S H K G L
Dog Lupus familis XP_534771 862 96765 N25 A K I L S R G N M S S H K G L
Cat Felis silvestris
Mouse Mus musculus Q9Z321 862 96931 N25 A K I L S R G N M S S H K G L
Rat Rattus norvegicus NP_001099331 862 96832 N25 A K I L S R G N M S S H K G L
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus NP_001006181 862 96669 N25 A K I L S R G N M S S R K G L
Frog Xenopus laevis NP_001082554 858 96526 N25 A K I L S R G N A S S R K G L
Zebra Danio Brachydanio rerio XP_002662012 861 96385 S25 A K I L S K G S C S T R K G L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster O96651 875 96955 R25 A G I L S N G R C T A K R G T
Honey Bee Apis mellifera XP_625165 851 95935 R25 A N I L S N G R C T S R K G L
Nematode Worm Caenorhab. elegans O61660 759 85420 T25 A A I L S N G T A N R R E G R
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P13099 656 74352
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 90 99.1 95.7 N.A. 95.5 94.6 N.A. N.A. 87.4 86.1 82.3 N.A. 57.2 64.3 30.5 N.A.
Protein Similarity: 100 90.1 99.7 97.5 N.A. 97.8 97.6 N.A. N.A. 93.9 93.2 91 N.A. 70 78.3 46.7 N.A.
P-Site Identity: 100 100 100 86.6 N.A. 86.6 86.6 N.A. N.A. 80 80 73.3 N.A. 40 60 40 N.A.
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. N.A. 93.3 86.6 86.6 N.A. 60 66.6 60 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 20.5 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 35.6 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 0 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 93 8 0 0 0 0 0 0 16 0 8 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 24 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 8 0 0 0 0 93 0 0 0 0 0 0 93 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 47 0 0 0 % H
% Ile: 0 0 93 0 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 70 0 0 0 8 0 0 0 0 0 8 77 0 0 % K
% Leu: 0 0 0 93 0 0 0 0 24 0 0 0 0 0 77 % L
% Met: 0 0 0 0 0 0 0 0 31 0 0 0 0 0 0 % M
% Asn: 0 8 0 0 0 24 0 39 0 8 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 62 0 16 0 0 8 39 8 0 8 % R
% Ser: 0 0 0 0 93 0 0 31 0 70 70 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 8 0 16 8 0 0 0 8 % T
% Val: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _