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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PGK1 All Species: 31.21
Human Site: S203 Identified Species: 49.05
UniProt: P00558 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P00558 NP_000282.1 417 44615 S203 Y F A K A L E S P E R P F L A
Chimpanzee Pan troglodytes XP_518531 417 44783 N203 Y F A K A L E N P V R P F L A
Rhesus Macaque Macaca mulatta XP_001100787 417 44568 S203 Y F A K A L E S P E R P F L A
Dog Lupus familis XP_849992 417 44444 C203 Y F A K A L E C P E R P F L A
Cat Felis silvestris
Mouse Mus musculus P09411 417 44532 S203 Y F A K A L E S P E R P F L A
Rat Rattus norvegicus P16617 417 44520 S203 Y F A K A L E S P E R P F L A
Wallaby Macropus eugenll P29408 417 44960 S203 Y F A K A L D S P E R P F L A
Platypus Ornith. anatinus XP_001507304 570 61329 S356 Y F A K A L E S P V R P F L A
Chicken Gallus gallus P51903 417 44698 S203 Y F A K A L E S P E R P F L A
Frog Xenopus laevis NP_001086928 417 44564 N203 Y F A K A L E N P E R P F L A
Zebra Danio Brachydanio rerio NP_998552 417 44672 K203 Y F A M A L E K P Q R P F L A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q01604 415 43843 K201 Y F S Q A L D K P P N P F L A
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P91427 417 44094 N202 Y F S K A L D N P A R P F L A
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P00560 416 44720 N201 Y F G K A L E N P T R P F L A
Red Bread Mold Neurospora crassa P38667 418 45038 S203 Y F A K V L E S P Q R P F L S
Conservation
Percent
Protein Identity: 100 87.2 99.7 96.4 N.A. 97.8 97.3 91.1 63.5 88.4 85.6 88.2 N.A. 70 N.A. 72.4 N.A.
Protein Similarity: 100 94.7 100 98.8 N.A. 99 98.8 95.6 70.3 96.1 94.2 93 N.A. 82 N.A. 83.6 N.A.
P-Site Identity: 100 86.6 100 93.3 N.A. 100 100 93.3 93.3 100 93.3 80 N.A. 60 N.A. 73.3 N.A.
P-Site Similarity: 100 93.3 100 93.3 N.A. 100 100 100 93.3 100 100 86.6 N.A. 80 N.A. 93.3 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 65.2 68.6
Protein Similarity: N.A. N.A. N.A. N.A. 76.9 80.3
P-Site Identity: N.A. N.A. N.A. N.A. 80 80
P-Site Similarity: N.A. N.A. N.A. N.A. 86.6 93.3
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 80 0 94 0 0 0 0 7 0 0 0 0 94 % A
% Cys: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 20 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 80 0 0 54 0 0 0 0 0 % E
% Phe: 0 100 0 0 0 0 0 0 0 0 0 0 100 0 0 % F
% Gly: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 0 0 87 0 0 0 14 0 0 0 0 0 0 0 % K
% Leu: 0 0 0 0 0 100 0 0 0 0 0 0 0 100 0 % L
% Met: 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 27 0 0 7 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 100 7 0 100 0 0 0 % P
% Gln: 0 0 0 7 0 0 0 0 0 14 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 0 0 0 0 94 0 0 0 0 % R
% Ser: 0 0 14 0 0 0 0 54 0 0 0 0 0 0 7 % S
% Thr: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % T
% Val: 0 0 0 0 7 0 0 0 0 14 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 100 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _