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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
PGK1
All Species:
33.94
Human Site:
S321
Identified Species:
53.33
UniProt:
P00558
Number Species:
14
Phosphosite Substitution
Charge Score:
-0.14
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
P00558
NP_000282.1
417
44615
S321
L
D
C
G
P
E
S
S
K
K
Y
A
E
A
V
Chimpanzee
Pan troglodytes
XP_518531
417
44783
N321
L
D
C
G
P
E
S
N
K
N
H
A
Q
V
V
Rhesus Macaque
Macaca mulatta
XP_001100787
417
44568
S321
L
D
C
G
P
E
S
S
K
K
Y
A
E
A
V
Dog
Lupus familis
XP_849992
417
44444
S321
L
D
C
G
P
E
S
S
K
K
Y
A
E
A
V
Cat
Felis silvestris
Mouse
Mus musculus
P09411
417
44532
S321
L
D
C
G
T
E
S
S
K
K
Y
A
E
A
V
Rat
Rattus norvegicus
P16617
417
44520
S321
L
D
C
G
T
E
S
S
K
K
Y
A
E
A
V
Wallaby
Macropus eugenll
P29408
417
44960
S321
L
D
C
G
P
K
S
S
K
K
Y
V
E
V
V
Platypus
Ornith. anatinus
XP_001507304
570
61329
S474
L
D
C
G
P
E
S
S
K
R
Y
A
E
A
V
Chicken
Gallus gallus
P51903
417
44698
V321
L
D
C
G
P
E
S
V
K
K
F
V
E
V
V
Frog
Xenopus laevis
NP_001086928
417
44564
V321
L
D
C
G
P
E
S
V
K
L
F
V
E
A
V
Zebra Danio
Brachydanio rerio
NP_998552
417
44672
S321
L
D
C
G
P
E
S
S
K
L
Y
A
E
A
V
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
Q01604
415
43843
R319
L
D
V
G
P
K
T
R
E
L
F
A
A
P
I
Honey Bee
Apis mellifera
Nematode Worm
Caenorhab. elegans
P91427
417
44094
S320
L
D
V
G
P
E
S
S
K
I
F
A
A
A
I
Sea Urchin
Strong. purpuratus
Poplar Tree
Populus trichocarpa
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
Baker's Yeast
Sacchar. cerevisiae
P00560
416
44720
R319
L
D
N
G
P
E
S
R
K
L
F
A
A
T
V
Red Bread Mold
Neurospora crassa
P38667
418
45038
V321
L
D
C
G
E
E
S
V
K
L
F
T
Q
A
I
Conservation
Percent
Protein Identity:
100
87.2
99.7
96.4
N.A.
97.8
97.3
91.1
63.5
88.4
85.6
88.2
N.A.
70
N.A.
72.4
N.A.
Protein Similarity:
100
94.7
100
98.8
N.A.
99
98.8
95.6
70.3
96.1
94.2
93
N.A.
82
N.A.
83.6
N.A.
P-Site Identity:
100
66.6
100
100
N.A.
93.3
93.3
80
93.3
73.3
73.3
93.3
N.A.
33.3
N.A.
66.6
N.A.
P-Site Similarity:
100
86.6
100
100
N.A.
93.3
93.3
86.6
100
80
80
93.3
N.A.
66.6
N.A.
80
N.A.
Percent
Protein Identity:
N.A.
N.A.
N.A.
N.A.
65.2
68.6
Protein Similarity:
N.A.
N.A.
N.A.
N.A.
76.9
80.3
P-Site Identity:
N.A.
N.A.
N.A.
N.A.
60
53.3
P-Site Similarity:
N.A.
N.A.
N.A.
N.A.
66.6
73.3
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
0
0
0
0
0
0
0
0
0
0
74
20
67
0
% A
% Cys:
0
0
80
0
0
0
0
0
0
0
0
0
0
0
0
% C
% Asp:
0
100
0
0
0
0
0
0
0
0
0
0
0
0
0
% D
% Glu:
0
0
0
0
7
87
0
0
7
0
0
0
67
0
0
% E
% Phe:
0
0
0
0
0
0
0
0
0
0
40
0
0
0
0
% F
% Gly:
0
0
0
100
0
0
0
0
0
0
0
0
0
0
0
% G
% His:
0
0
0
0
0
0
0
0
0
0
7
0
0
0
0
% H
% Ile:
0
0
0
0
0
0
0
0
0
7
0
0
0
0
20
% I
% Lys:
0
0
0
0
0
14
0
0
94
47
0
0
0
0
0
% K
% Leu:
100
0
0
0
0
0
0
0
0
34
0
0
0
0
0
% L
% Met:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% M
% Asn:
0
0
7
0
0
0
0
7
0
7
0
0
0
0
0
% N
% Pro:
0
0
0
0
80
0
0
0
0
0
0
0
0
7
0
% P
% Gln:
0
0
0
0
0
0
0
0
0
0
0
0
14
0
0
% Q
% Arg:
0
0
0
0
0
0
0
14
0
7
0
0
0
0
0
% R
% Ser:
0
0
0
0
0
0
94
60
0
0
0
0
0
0
0
% S
% Thr:
0
0
0
0
14
0
7
0
0
0
0
7
0
7
0
% T
% Val:
0
0
14
0
0
0
0
20
0
0
0
20
0
20
80
% V
% Trp:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% W
% Tyr:
0
0
0
0
0
0
0
0
0
0
54
0
0
0
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _