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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TYMS All Species: 42.73
Human Site: Y33 Identified Species: 62.67
UniProt: P04818 Number Species: 15
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P04818 NP_001062.1 313 35716 Y33 P P H G E L Q Y L G Q I Q H I
Chimpanzee Pan troglodytes XP_001150437 313 35656 Y33 P P H G E L Q Y L G Q I E H I
Rhesus Macaque Macaca mulatta XP_001089274 313 35614 Y33 P P H G E L Q Y L G Q I E H I
Dog Lupus familis XP_533309 325 36767 Y34 P P H G E L Q Y L G Q V E H I
Cat Felis silvestris
Mouse Mus musculus P07607 307 34940 Y27 P R H G E L Q Y L R Q V E H I
Rat Rattus norvegicus P45352 307 34999 Y27 P Q H G E L Q Y L R Q V E H I
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001508361 313 35234 Y33 P P H G E L Q Y L G Q V R H I
Chicken Gallus gallus XP_419147 298 34062 Q21 G E P Q Y L Q Q V R H I L Q H
Frog Xenopus laevis NP_001096659 309 35145 Y29 E N R D E L Q Y L D Q I K Y I
Zebra Danio Brachydanio rerio NP_571835 319 36295 Y39 L F C D E R G Y L S L V E F I
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster O76511 321 36638 Y39 V N R D E M H Y L D L L R H I
Honey Bee Apis mellifera XP_624369 305 35528 Y24 K K H E E Y Q Y L N L I E K I
Nematode Worm Caenorhab. elegans NP_491532 312 35434 Y31 L N Q D E Y K Y L K Q V E Q I
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays O81395 521 58947 Y241 D R H E E Y Q Y L N L V E D I
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q05763 565 63190 Y285 D R H E E Y L Y L N L V K E I
Baker's Yeast Sacchar. cerevisiae P06785 304 35029 D22 D L C K R I I D E G E F R P D
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99 97.7 84.6 N.A. 88.8 88.5 N.A. 82.4 81.1 79.5 74.9 N.A. 62.9 65.5 62.6 N.A.
Protein Similarity: 100 100 99 89.5 N.A. 93.6 93.2 N.A. 91 87.8 87.5 84.9 N.A. 76 77.9 73.8 N.A.
P-Site Identity: 100 93.3 93.3 86.6 N.A. 73.3 73.3 N.A. 86.6 20 53.3 26.6 N.A. 33.3 46.6 33.3 N.A.
P-Site Similarity: 100 100 100 100 N.A. 86.6 86.6 N.A. 100 26.6 66.6 40 N.A. 53.3 53.3 53.3 N.A.
Percent
Protein Identity: N.A. 39.7 N.A. 37.1 60 N.A.
Protein Similarity: N.A. 48.1 N.A. 44.4 73.8 N.A.
P-Site Identity: N.A. 40 N.A. 33.3 6.6 N.A.
P-Site Similarity: N.A. 53.3 N.A. 46.6 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 13 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 19 0 0 25 0 0 0 7 0 13 0 0 0 7 7 % D
% Glu: 7 7 0 19 88 0 0 0 7 0 7 0 57 7 0 % E
% Phe: 0 7 0 0 0 0 0 0 0 0 0 7 0 7 0 % F
% Gly: 7 0 0 44 0 0 7 0 0 38 0 0 0 0 0 % G
% His: 0 0 63 0 0 0 7 0 0 0 7 0 0 50 7 % H
% Ile: 0 0 0 0 0 7 7 0 0 0 0 38 0 0 88 % I
% Lys: 7 7 0 7 0 0 7 0 0 7 0 0 13 7 0 % K
% Leu: 13 7 0 0 0 57 7 0 88 0 32 7 7 0 0 % L
% Met: 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 19 0 0 0 0 0 0 0 19 0 0 0 0 0 % N
% Pro: 44 32 7 0 0 0 0 0 0 0 0 0 0 7 0 % P
% Gln: 0 7 7 7 0 0 69 7 0 0 57 0 7 13 0 % Q
% Arg: 0 19 13 0 7 7 0 0 0 19 0 0 19 0 0 % R
% Ser: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % T
% Val: 7 0 0 0 0 0 0 0 7 0 0 50 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 7 25 0 88 0 0 0 0 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _