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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKM All Species: 10.3
Human Site: S400 Identified Species: 18.89
UniProt: P08237 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P08237 NP_000280.1 780 85183 S400 P P V S K S G S H T V A V M N
Chimpanzee Pan troglodytes XP_001150233 780 84936 F400 P P K E K S N F S L A I L N V
Rhesus Macaque Macaca mulatta XP_001096244 851 93251 S471 P P V S K S G S H T V A V M N
Dog Lupus familis XP_544922 751 82459 M385 V G A P A A G M N A A V R S A
Cat Felis silvestris
Mouse Mus musculus P47857 780 85250 L400 P P V S K G G L H T V A V M N
Rat Rattus norvegicus P47858 780 85541 L400 P P V S K G G L H T V A V M N
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511162 864 94802 S480 P D A E L P T S N F N V A V L
Chicken Gallus gallus NP_989554 769 83907 H388 P P S T K S G H T L A V L N V
Frog Xenopus laevis NP_001086921 808 88294 Y429 P P S A K S G Y N L A V L N V
Zebra Danio Brachydanio rerio NP_001004575 784 85614 I402 P P D K K S N I N V A I L N V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52034 788 86630 A402 P P K E N F D A D G K G I E G
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q27483 756 83282 T403 S T T M P S R T K T F A V V H
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P16862 959 104599 R586 P K L P K D K R L K I A I V N
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 68.5 90.4 67.6 N.A. 97.8 96.6 N.A. 65.2 81.2 81.4 77.8 N.A. 58.7 N.A. 39.6 N.A.
Protein Similarity: 100 82.8 91.1 81.7 N.A. 99 98.2 N.A. 76.2 90.1 90.3 88.7 N.A. 75.7 N.A. 57.6 N.A.
P-Site Identity: 100 26.6 100 6.6 N.A. 86.6 86.6 N.A. 13.3 33.3 33.3 26.6 N.A. 13.3 N.A. 26.6 N.A.
P-Site Similarity: 100 33.3 100 20 N.A. 86.6 86.6 N.A. 26.6 46.6 53.3 40 N.A. 26.6 N.A. 46.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 34.7 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 50.8 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 26.6 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 53.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 16 8 8 8 0 8 0 8 39 47 8 0 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 8 8 0 0 8 8 0 8 0 0 0 0 0 0 % D
% Glu: 0 0 0 24 0 0 0 0 0 0 0 0 0 8 0 % E
% Phe: 0 0 0 0 0 8 0 8 0 8 8 0 0 0 0 % F
% Gly: 0 8 0 0 0 16 54 0 0 8 0 8 0 0 8 % G
% His: 0 0 0 0 0 0 0 8 31 0 0 0 0 0 8 % H
% Ile: 0 0 0 0 0 0 0 8 0 0 8 16 16 0 0 % I
% Lys: 0 8 16 8 70 0 8 0 8 8 8 0 0 0 0 % K
% Leu: 0 0 8 0 8 0 0 16 8 24 0 0 31 0 8 % L
% Met: 0 0 0 8 0 0 0 8 0 0 0 0 0 31 0 % M
% Asn: 0 0 0 0 8 0 16 0 31 0 8 0 0 31 39 % N
% Pro: 85 70 0 16 8 8 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 0 0 8 8 0 0 0 0 8 0 0 % R
% Ser: 8 0 16 31 0 54 0 24 8 0 0 0 0 8 0 % S
% Thr: 0 8 8 8 0 0 8 8 8 39 0 0 0 0 0 % T
% Val: 8 0 31 0 0 0 0 0 0 8 31 31 39 24 31 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _