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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKM All Species: 29.7
Human Site: T704 Identified Species: 54.44
UniProt: P08237 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.17
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P08237 NP_000280.1 780 85183 T704 N G R I F A N T P D S G C V L
Chimpanzee Pan troglodytes XP_001150233 780 84936 A703 K G R V F A N A P D S A C V I
Rhesus Macaque Macaca mulatta XP_001096244 851 93251 T775 N G R I F A N T P D S G C V L
Dog Lupus familis XP_544922 751 82459 A674 N G R V F A N A P D S A C V I
Cat Felis silvestris
Mouse Mus musculus P47857 780 85250 T704 N G R I F A N T P D S G C V L
Rat Rattus norvegicus P47858 780 85541 T704 N G R I F A N T P D S G C V L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001511162 864 94802 T785 K G K I F A N T D D S V C L L
Chicken Gallus gallus NP_989554 769 83907 T692 H G R I F A N T A D S A C L L
Frog Xenopus laevis NP_001086921 808 88294 T732 H G R I F A N T P D S A C L L
Zebra Danio Brachydanio rerio NP_001004575 784 85614 T705 H G R I F A N T P D S A C V L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52034 788 86630 K713 A N G V V N C K S P D T A T L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q27483 756 83282 R699 N P V K M G D R D C C V I G L
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P16862 959 104599 F896 A R A A E E N F N A D D K T I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 68.5 90.4 67.6 N.A. 97.8 96.6 N.A. 65.2 81.2 81.4 77.8 N.A. 58.7 N.A. 39.6 N.A.
Protein Similarity: 100 82.8 91.1 81.7 N.A. 99 98.2 N.A. 76.2 90.1 90.3 88.7 N.A. 75.7 N.A. 57.6 N.A.
P-Site Identity: 100 66.6 100 73.3 N.A. 100 100 N.A. 66.6 73.3 80 86.6 N.A. 6.6 N.A. 13.3 N.A.
P-Site Similarity: 100 80 100 86.6 N.A. 100 100 N.A. 80 86.6 93.3 93.3 N.A. 13.3 N.A. 20 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 34.7 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 50.8 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 16 0 8 8 0 77 0 16 8 8 0 39 8 0 0 % A
% Cys: 0 0 0 0 0 0 8 0 0 8 8 0 77 0 0 % C
% Asp: 0 0 0 0 0 0 8 0 16 77 16 8 0 0 0 % D
% Glu: 0 0 0 0 8 8 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 77 0 0 8 0 0 0 0 0 0 0 % F
% Gly: 0 77 8 0 0 8 0 0 0 0 0 31 0 8 0 % G
% His: 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 62 0 0 0 0 0 0 0 0 8 0 24 % I
% Lys: 16 0 8 8 0 0 0 8 0 0 0 0 8 0 0 % K
% Leu: 0 0 0 0 0 0 0 0 0 0 0 0 0 24 77 % L
% Met: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 47 8 0 0 0 8 85 0 8 0 0 0 0 0 0 % N
% Pro: 0 8 0 0 0 0 0 0 62 8 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 8 70 0 0 0 0 8 0 0 0 0 0 0 0 % R
% Ser: 0 0 0 0 0 0 0 0 8 0 77 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 62 0 0 0 8 0 16 0 % T
% Val: 0 0 8 24 8 0 0 0 0 0 0 16 0 54 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _