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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: ARAF All Species: 24.85
Human Site: S535 Identified Species: 45.56
UniProt: P10398 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.25
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P10398 NP_001645.1 606 67585 S535 S P D L S K I S S N C P K A M
Chimpanzee Pan troglodytes XP_001155232 648 73032 Y574 S P D L S K L Y K N C P K A M
Rhesus Macaque Macaca mulatta XP_001094928 606 67485 S535 S P D L S K I S S N C P K A M
Dog Lupus familis XP_548976 597 66626 S526 S P D L S K I S S N C P K A M
Cat Felis silvestris
Mouse Mus musculus P04627 604 67563 F533 S P D L S K I F S N C P K A M
Rat Rattus norvegicus P14056 604 67533 F533 S P D L S K I F S N C P K A M
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516512 799 90020 R714 S P D L S K V R S N C P K A M
Chicken Gallus gallus P05625 647 73106 Y574 S P D L S K L Y K N C P K A M
Frog Xenopus laevis P09560 638 71941 Y565 V P D L S K L Y K N C P K A M
Zebra Danio Brachydanio rerio NP_991306 608 68644 Y537 S P D L S K L Y S N C P K S M
Tiger Blowfish Takifugu rubipres NP_001033077 573 64648 N503 S G S L P Y S N I N N R D Q I
Fruit Fly Dros. melanogaster P11346 782 88616 R697 R P D M S Q V R S D A P Q A L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q07292 813 90389 R711 R P D R S K I R H D T P K S M
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 58.1 97 92.7 N.A. 95.2 95.5 N.A. 47.6 58.1 57.8 67.4 63.5 34.5 N.A. 32.8 N.A.
Protein Similarity: 100 70.8 97.6 94 N.A. 97 97 N.A. 57.7 71.2 70.6 77.8 74.2 48.8 N.A. 48 N.A.
P-Site Identity: 100 80 100 100 N.A. 93.3 93.3 N.A. 86.6 80 73.3 80 20 40 N.A. 53.3 N.A.
P-Site Similarity: 100 86.6 100 100 N.A. 93.3 93.3 N.A. 93.3 86.6 80 93.3 33.3 80 N.A. 66.6 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 8 0 0 77 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 77 0 0 0 0 % C
% Asp: 0 0 93 0 0 0 0 0 0 16 0 0 8 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 16 0 0 0 0 0 0 0 % F
% Gly: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 47 0 8 0 0 0 0 0 8 % I
% Lys: 0 0 0 0 0 85 0 0 24 0 0 0 85 0 0 % K
% Leu: 0 0 0 85 0 0 31 0 0 0 0 0 0 0 8 % L
% Met: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 85 % M
% Asn: 0 0 0 0 0 0 0 8 0 85 8 0 0 0 0 % N
% Pro: 0 93 0 0 8 0 0 0 0 0 0 93 0 0 0 % P
% Gln: 0 0 0 0 0 8 0 0 0 0 0 0 8 8 0 % Q
% Arg: 16 0 0 8 0 0 0 24 0 0 0 8 0 0 0 % R
% Ser: 77 0 8 0 93 0 8 24 62 0 0 0 0 16 0 % S
% Thr: 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 % T
% Val: 8 0 0 0 0 0 16 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 8 0 31 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _