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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1 All Species: 25.76
Human Site: S47 Identified Species: 43.59
UniProt: P11387 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11387 NP_003277.1 765 90726 S47 K E K D R E K S K H S N S E H
Chimpanzee Pan troglodytes A9Q1D5 601 69739 P20 L T L L G E V P R R P A S R G
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 S49 K E K D R E K S K H S N S E H
Dog Lupus familis XP_534420 1004 115318 S283 K E K D R E K S K H S N S E H
Cat Felis silvestris
Mouse Mus musculus Q04750 767 90857 S49 K D K D R E K S K H S N S E H
Rat Rattus norvegicus Q9WUL0 767 90741 S49 K D K D R E K S K H S N S E H
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509816 781 92543 K65 E K D R E K S K H S N S E H K
Chicken Gallus gallus
Frog Xenopus laevis P41512 829 98212 H63 E K K H K D K H K N N D K H R
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 S87 R D K D R H K S S S S S S K H
Honey Bee Apis mellifera XP_396203 973 114099 S177 K E K D K D K S K D K D K Y K
Nematode Worm Caenorhab. elegans O17966 806 94011 A110 K K N N K K K A Q E S S E D D
Sea Urchin Strong. purpuratus XP_793285 879 100968 K167 K D I N S K H K E H S S S K H
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 D185 S M F R K K I D S G M S G G N
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 S39 V A S M N S A S L Q D E A E P
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 54.1 99 74.8 N.A. 97 97 N.A. 90.4 N.A. 75 N.A. N.A. 49.6 53.5 54.8 45.3
Protein Similarity: 100 67.4 99.6 75.8 N.A. 98.6 98.6 N.A. 93.8 N.A. 82.7 N.A. N.A. 62.3 64.4 69.1 58
P-Site Identity: 100 13.3 100 100 N.A. 93.3 93.3 N.A. 0 N.A. 20 N.A. N.A. 53.3 46.6 20 33.3
P-Site Similarity: 100 20 100 100 N.A. 100 100 N.A. 33.3 N.A. 66.6 N.A. N.A. 80 66.6 73.3 73.3
Percent
Protein Identity: N.A. N.A. N.A. 36.6 46.8 N.A.
Protein Similarity: N.A. N.A. N.A. 53.3 63.9 N.A.
P-Site Identity: N.A. N.A. N.A. 0 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 0 0 0 8 8 0 0 0 8 8 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 29 8 50 0 15 0 8 0 8 8 15 0 8 8 % D
% Glu: 15 29 0 0 8 43 0 0 8 8 0 8 15 43 0 % E
% Phe: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 8 0 0 0 0 8 0 0 8 8 8 % G
% His: 0 0 0 8 0 8 8 8 8 43 0 0 0 15 50 % H
% Ile: 0 0 8 0 0 0 8 0 0 0 0 0 0 0 0 % I
% Lys: 58 22 58 0 29 29 65 15 50 0 8 0 15 15 15 % K
% Leu: 8 0 8 8 0 0 0 0 8 0 0 0 0 0 0 % L
% Met: 0 8 0 8 0 0 0 0 0 0 8 0 0 0 0 % M
% Asn: 0 0 8 15 8 0 0 0 0 8 15 36 0 0 8 % N
% Pro: 0 0 0 0 0 0 0 8 0 0 8 0 0 0 8 % P
% Gln: 0 0 0 0 0 0 0 0 8 8 0 0 0 0 0 % Q
% Arg: 8 0 0 15 43 0 0 0 8 8 0 0 0 8 8 % R
% Ser: 8 0 8 0 8 8 8 58 15 15 58 36 58 0 0 % S
% Thr: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % T
% Val: 8 0 0 0 0 0 8 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _