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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1 All Species: 28.18
Human Site: S74 Identified Species: 47.69
UniProt: P11387 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11387 NP_003277.1 765 90726 S74 T K H K D G S S E K H K D K H
Chimpanzee Pan troglodytes A9Q1D5 601 69739 H47 A R W E K E K H E D G V K W R
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 S76 T K H K D G S S E K H K D K H
Dog Lupus familis XP_534420 1004 115318 S310 T K H K D G S S E K H K D K H
Cat Felis silvestris
Mouse Mus musculus Q04750 767 90857 S76 T K H K D G S S E K H K D K H
Rat Rattus norvegicus Q9WUL0 767 90741 S76 T K H K D G S S D K H K D K H
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509816 781 92543 S92 T K H K D G S S E K H K D K H
Chicken Gallus gallus
Frog Xenopus laevis P41512 829 98212 G90 E K H R D K N G E K H R D G E
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 S114 S H R S G S S S S H K D K D G
Honey Bee Apis mellifera XP_396203 973 114099 K204 D K D K N I S K D K E K E R K
Nematode Worm Caenorhab. elegans O17966 806 94011 D137 V K P Q I H S D D E L E E E D
Sea Urchin Strong. purpuratus XP_793285 879 100968 P194 V E L K K E K P E V K E E M A
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 T212 K L H Q N G S T V K N E V P N
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 T66 K K T K K I K T E P V Q S S S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 54.1 99 74.8 N.A. 97 97 N.A. 90.4 N.A. 75 N.A. N.A. 49.6 53.5 54.8 45.3
Protein Similarity: 100 67.4 99.6 75.8 N.A. 98.6 98.6 N.A. 93.8 N.A. 82.7 N.A. N.A. 62.3 64.4 69.1 58
P-Site Identity: 100 6.6 100 100 N.A. 100 93.3 N.A. 100 N.A. 46.6 N.A. N.A. 13.3 33.3 13.3 13.3
P-Site Similarity: 100 20 100 100 N.A. 100 100 N.A. 100 N.A. 66.6 N.A. N.A. 20 60 53.3 33.3
Percent
Protein Identity: N.A. N.A. N.A. 36.6 46.8 N.A.
Protein Similarity: N.A. N.A. N.A. 53.3 63.9 N.A.
P-Site Identity: N.A. N.A. N.A. 26.6 20 N.A.
P-Site Similarity: N.A. N.A. N.A. 66.6 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 8 0 8 0 50 0 0 8 22 8 0 8 50 8 8 % D
% Glu: 8 8 0 8 0 15 0 0 65 8 8 22 22 8 8 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 8 50 0 8 0 0 8 0 0 8 8 % G
% His: 0 8 58 0 0 8 0 8 0 8 50 0 0 0 43 % H
% Ile: 0 0 0 0 8 15 0 0 0 0 0 0 0 0 0 % I
% Lys: 15 72 0 65 22 8 22 8 0 65 15 50 15 43 8 % K
% Leu: 0 8 8 0 0 0 0 0 0 0 8 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % M
% Asn: 0 0 0 0 15 0 8 0 0 0 8 0 0 0 8 % N
% Pro: 0 0 8 0 0 0 0 8 0 8 0 0 0 8 0 % P
% Gln: 0 0 0 15 0 0 0 0 0 0 0 8 0 0 0 % Q
% Arg: 0 8 8 8 0 0 0 0 0 0 0 8 0 8 8 % R
% Ser: 8 0 0 8 0 8 72 50 8 0 0 0 8 8 8 % S
% Thr: 43 0 8 0 0 0 0 15 0 0 0 0 0 0 0 % T
% Val: 15 0 0 0 0 0 0 0 8 8 8 8 8 0 0 % V
% Trp: 0 0 8 0 0 0 0 0 0 0 0 0 0 8 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _