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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1 All Species: 26.06
Human Site: T295 Identified Species: 44.1
UniProt: P11387 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11387 NP_003277.1 765 90726 T295 N E E K N I I T N L S K C D F
Chimpanzee Pan troglodytes A9Q1D5 601 69739 G207 R G D H P K M G M L K R R I M
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 T297 N E E K N I I T N L S K C D F
Dog Lupus familis XP_534420 1004 115318 T534 N E E K N I I T N L S K C D F
Cat Felis silvestris
Mouse Mus musculus Q04750 767 90857 T297 N D E K N T I T N L S K C D F
Rat Rattus norvegicus Q9WUL0 767 90741 T297 N D E K N T I T N L S K C D F
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509816 781 92543 T311 N E E K S N I T N L S K C D F
Chicken Gallus gallus
Frog Xenopus laevis P41512 829 98212 T351 T D E R N L I T N L S K C D F
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 K518 P R E R E I I K D F R K C N F
Honey Bee Apis mellifera XP_396203 973 114099 V521 E S E R T K I V D L S K C N F
Nematode Worm Caenorhab. elegans O17966 806 94011 H337 V E E R E R I H D L K K C D F
Sea Urchin Strong. purpuratus XP_793285 879 100968 N439 Y T T K D V F N N N F Y R D W
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 Q445 L G K K H V I Q K L D D C D F
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 K227 P L N G I E I K E F S R C D F
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 54.1 99 74.8 N.A. 97 97 N.A. 90.4 N.A. 75 N.A. N.A. 49.6 53.5 54.8 45.3
Protein Similarity: 100 67.4 99.6 75.8 N.A. 98.6 98.6 N.A. 93.8 N.A. 82.7 N.A. N.A. 62.3 64.4 69.1 58
P-Site Identity: 100 6.6 100 100 N.A. 86.6 86.6 N.A. 86.6 N.A. 73.3 N.A. N.A. 40 46.6 53.3 20
P-Site Similarity: 100 26.6 100 100 N.A. 93.3 93.3 N.A. 93.3 N.A. 93.3 N.A. N.A. 60 66.6 66.6 40
Percent
Protein Identity: N.A. N.A. N.A. 36.6 46.8 N.A.
Protein Similarity: N.A. N.A. N.A. 53.3 63.9 N.A.
P-Site Identity: N.A. N.A. N.A. 40 33.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 60 40 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 86 0 0 % C
% Asp: 0 22 8 0 8 0 0 0 22 0 8 8 0 79 0 % D
% Glu: 8 36 72 0 15 8 0 0 8 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 8 0 0 15 8 0 0 0 86 % F
% Gly: 0 15 0 8 0 0 0 8 0 0 0 0 0 0 0 % G
% His: 0 0 0 8 8 0 0 8 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 8 29 86 0 0 0 0 0 0 8 0 % I
% Lys: 0 0 8 58 0 15 0 15 8 0 15 72 0 0 0 % K
% Leu: 8 8 0 0 0 8 0 0 0 79 0 0 0 0 0 % L
% Met: 0 0 0 0 0 0 8 0 8 0 0 0 0 0 8 % M
% Asn: 43 0 8 0 43 8 0 8 58 8 0 0 0 15 0 % N
% Pro: 15 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 % Q
% Arg: 8 8 0 29 0 8 0 0 0 0 8 15 15 0 0 % R
% Ser: 0 8 0 0 8 0 0 0 0 0 65 0 0 0 0 % S
% Thr: 8 8 8 0 8 15 0 50 0 0 0 0 0 0 0 % T
% Val: 8 0 0 0 0 15 0 8 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % W
% Tyr: 8 0 0 0 0 0 0 0 0 0 0 8 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _