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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1 All Species: 32.12
Human Site: T313 Identified Species: 54.36
UniProt: P11387 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.15
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11387 NP_003277.1 765 90726 T313 S Q Y F K A Q T E A R K Q M S
Chimpanzee Pan troglodytes A9Q1D5 601 69739 D225 V V I N C S R D S K I P E P P
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 S315 S Q Y F K A Q S E A R K Q M S
Dog Lupus familis XP_534420 1004 115318 S552 S Q Y F K A Q S E A R K Q M S
Cat Felis silvestris
Mouse Mus musculus Q04750 767 90857 S315 S Q Y F K A Q S E A R K Q M S
Rat Rattus norvegicus Q9WUL0 767 90741 S315 S Q Y F K A Q S E A R K Q M S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509816 781 92543 S329 S Q Y F K A Q S E A R K Q M S
Chicken Gallus gallus
Frog Xenopus laevis P41512 829 98212 S369 S L Y F K E Q S E A R K N M T
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 S536 F N Y F Q A E S E K R K A A S
Honey Bee Apis mellifera XP_396203 973 114099 S539 H A Y F I Q K S E E R K A M T
Nematode Worm Caenorhab. elegans O17966 806 94011 R355 D A Y Q K E Q R E I R K A M T
Sea Urchin Strong. purpuratus XP_793285 879 100968 I457 M T H E E K A I I K D M K K C
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 K463 Y E W H L E E K E K K K Q M S
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 K245 F D Y F Q L Q K E Q K K Q L T
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 54.1 99 74.8 N.A. 97 97 N.A. 90.4 N.A. 75 N.A. N.A. 49.6 53.5 54.8 45.3
Protein Similarity: 100 67.4 99.6 75.8 N.A. 98.6 98.6 N.A. 93.8 N.A. 82.7 N.A. N.A. 62.3 64.4 69.1 58
P-Site Identity: 100 0 93.3 93.3 N.A. 93.3 93.3 N.A. 93.3 N.A. 66.6 N.A. N.A. 46.6 40 46.6 0
P-Site Similarity: 100 20 100 100 N.A. 100 100 N.A. 100 N.A. 80 N.A. N.A. 66.6 60 53.3 20
Percent
Protein Identity: N.A. N.A. N.A. 36.6 46.8 N.A.
Protein Similarity: N.A. N.A. N.A. 53.3 63.9 N.A.
P-Site Identity: N.A. N.A. N.A. 33.3 40 N.A.
P-Site Similarity: N.A. N.A. N.A. 60 66.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 15 0 0 0 50 8 0 0 50 0 0 22 8 0 % A
% Cys: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 8 % C
% Asp: 8 8 0 0 0 0 0 8 0 0 8 0 0 0 0 % D
% Glu: 0 8 0 8 8 22 15 0 86 8 0 0 8 0 0 % E
% Phe: 15 0 0 72 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 8 0 8 8 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 8 0 8 0 0 8 8 8 8 0 0 0 0 % I
% Lys: 0 0 0 0 58 8 8 15 0 29 15 86 8 8 0 % K
% Leu: 0 8 0 0 8 8 0 0 0 0 0 0 0 8 0 % L
% Met: 8 0 0 0 0 0 0 0 0 0 0 8 0 72 0 % M
% Asn: 0 8 0 8 0 0 0 0 0 0 0 0 8 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 8 0 8 8 % P
% Gln: 0 43 0 8 15 8 65 0 0 8 0 0 58 0 0 % Q
% Arg: 0 0 0 0 0 0 8 8 0 0 72 0 0 0 0 % R
% Ser: 50 0 0 0 0 8 0 58 8 0 0 0 0 0 58 % S
% Thr: 0 8 0 0 0 0 0 8 0 0 0 0 0 0 29 % T
% Val: 8 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 8 0 79 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _