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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP1 All Species: 38.79
Human Site: Y231 Identified Species: 65.64
UniProt: P11387 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11387 NP_003277.1 765 90726 Y231 G P V F A P P Y E P L P E N V
Chimpanzee Pan troglodytes A9Q1D5 601 69739 F145 F T E I H R Y F V D K A A A R
Rhesus Macaque Macaca mulatta XP_001088021 767 90935 Y233 G P V F A P P Y E P L P E S V
Dog Lupus familis XP_534420 1004 115318 Y470 G P V F A P P Y E P L P E S V
Cat Felis silvestris
Mouse Mus musculus Q04750 767 90857 Y233 G P V F A P P Y E P L P E S V
Rat Rattus norvegicus Q9WUL0 767 90741 Y233 G P V F A P P Y E P L P E G V
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509816 781 92543 Y247 G P V F A P P Y E P L P E N V
Chicken Gallus gallus
Frog Xenopus laevis P41512 829 98212 Y287 G P V F A P P Y E P V P D N V
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P30189 972 111670 Y454 G P V F A P R Y E R V P R N V
Honey Bee Apis mellifera XP_396203 973 114099 Y457 G P V F A P P Y E P L P P D V
Nematode Worm Caenorhab. elegans O17966 806 94011 Y273 G P L F A P P Y I P L P S H V
Sea Urchin Strong. purpuratus XP_793285 879 100968 H375 R G A K E T P H P E G V K W M
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30181 916 102780 P383 H N G V I F P P P Y K P H G I
Baker's Yeast Sacchar. cerevisiae P04786 769 89977 H165 P Y Q P L P S H I K L Y Y D G
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 54.1 99 74.8 N.A. 97 97 N.A. 90.4 N.A. 75 N.A. N.A. 49.6 53.5 54.8 45.3
Protein Similarity: 100 67.4 99.6 75.8 N.A. 98.6 98.6 N.A. 93.8 N.A. 82.7 N.A. N.A. 62.3 64.4 69.1 58
P-Site Identity: 100 0 93.3 93.3 N.A. 93.3 93.3 N.A. 100 N.A. 86.6 N.A. N.A. 73.3 86.6 73.3 6.6
P-Site Similarity: 100 6.6 100 100 N.A. 100 93.3 N.A. 100 N.A. 100 N.A. N.A. 80 93.3 86.6 26.6
Percent
Protein Identity: N.A. N.A. N.A. 36.6 46.8 N.A.
Protein Similarity: N.A. N.A. N.A. 53.3 63.9 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. 20 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 8 0 72 0 0 0 0 0 0 8 8 8 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 8 0 0 8 15 0 % D
% Glu: 0 0 8 0 8 0 0 0 65 8 0 0 43 0 0 % E
% Phe: 8 0 0 72 0 8 0 8 0 0 0 0 0 0 0 % F
% Gly: 72 8 8 0 0 0 0 0 0 0 8 0 0 15 8 % G
% His: 8 0 0 0 8 0 0 15 0 0 0 0 8 8 0 % H
% Ile: 0 0 0 8 8 0 0 0 15 0 0 0 0 0 8 % I
% Lys: 0 0 0 8 0 0 0 0 0 8 15 0 8 0 0 % K
% Leu: 0 0 8 0 8 0 0 0 0 0 65 0 0 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 % M
% Asn: 0 8 0 0 0 0 0 0 0 0 0 0 0 29 0 % N
% Pro: 8 72 0 8 0 79 79 8 15 65 0 79 8 0 0 % P
% Gln: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 8 0 0 0 0 8 8 0 0 8 0 0 8 0 8 % R
% Ser: 0 0 0 0 0 0 8 0 0 0 0 0 8 22 0 % S
% Thr: 0 8 0 0 0 8 0 0 0 0 0 0 0 0 0 % T
% Val: 0 0 65 8 0 0 0 0 8 0 15 8 0 0 72 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % W
% Tyr: 0 8 0 0 0 0 8 72 0 8 0 8 8 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _