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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 7.27
Human Site: S1365 Identified Species: 11.43
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 S1365 T K T S P K L S N K E L K P Q
Chimpanzee Pan troglodytes XP_516332 1634 184611 N1412 V K A S P I T N D G E D E F V
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 N1398 V K A S P I T N D G E D E F V
Dog Lupus familis XP_537646 1532 174610 A1366 K A S P K R T A K E L K P Q K
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 T1361 A K I P P K N T K K A L K T Q
Rat Rattus norvegicus P41516 1526 173202 T1361 T K M P P K N T K K A L K P Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 R1395 A S R G A I S R T K A D P G E
Chicken Gallus gallus O42130 1553 174974 D1387 P K E K P L P D A V P V R V Q
Frog Xenopus laevis NP_001082502 1579 178601 I1380 S F V I A K P I K K P R A A P
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 S1374 E P S A A P D S D V D S P P P
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 P1281 G K Q I K A E P D A S G D E V
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 F1350 S E E S D V E F D E G I D F D
Sea Urchin Strong. purpuratus XP_783546 1448 163750 M1282 P K D L D I K M E E G S E L S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 E1299 A K E V E E D E M L D L A Q R
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 N1263 T K T E E E E N A P S S T S S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 26.6 26.6 0 N.A. 46.6 60 N.A. 6.6 20 13.3 13.3 N.A. 6.6 N.A. 6.6 6.6
P-Site Similarity: 100 46.6 46.6 33.3 N.A. 53.3 66.6 N.A. 13.3 33.3 20 40 N.A. 13.3 N.A. 40 20
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 20 N.A.
P-Site Similarity: N.A. N.A. N.A. 33.3 33.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 20 7 14 7 20 7 0 7 14 7 20 0 14 7 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 7 0 14 0 14 7 34 0 14 20 14 0 7 % D
% Glu: 7 7 20 7 14 14 20 7 7 20 20 0 20 7 7 % E
% Phe: 0 7 0 0 0 0 0 7 0 0 0 0 0 20 0 % F
% Gly: 7 0 0 7 0 0 0 0 0 14 14 7 0 7 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 7 14 0 27 0 7 0 0 0 7 0 0 0 % I
% Lys: 7 67 0 7 14 27 7 0 27 34 0 7 20 0 7 % K
% Leu: 0 0 0 7 0 7 7 0 0 7 7 27 0 7 0 % L
% Met: 0 0 7 0 0 0 0 7 7 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 14 20 7 0 0 0 0 0 0 % N
% Pro: 14 7 0 20 40 7 14 7 0 7 14 0 20 20 14 % P
% Gln: 0 0 7 0 0 0 0 0 0 0 0 0 0 14 27 % Q
% Arg: 0 0 7 0 0 7 0 7 0 0 0 7 7 0 7 % R
% Ser: 14 7 14 27 0 0 7 14 0 0 14 20 0 7 14 % S
% Thr: 20 0 14 0 0 0 20 14 7 0 0 0 7 7 0 % T
% Val: 14 0 7 7 0 7 0 0 0 14 0 7 0 7 20 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _