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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 9.09
Human Site: S1374 Identified Species: 14.29
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 S1374 K E L K P Q K S V V S D L E A
Chimpanzee Pan troglodytes XP_516332 1634 184611 S1421 G E D E F V P S D G L D K D E
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 S1407 G E D E F V P S D G L D K D E
Dog Lupus familis XP_537646 1532 174610 A1375 E L K P Q K S A P P V M D L D
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 S1370 K A L K T Q G S S M S V V D L
Rat Rattus norvegicus P41516 1526 173202 S1370 K A L K P Q K S S T S V D L E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 Q1404 K A D P G E P Q G K V A K E E
Chicken Gallus gallus O42130 1553 174974 V1396 V P V R V Q N V A A E S A S Q
Frog Xenopus laevis NP_001082502 1579 178601 K1389 K P R A A P K K A E K A K K T
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 V1383 V D S P P P P V V K K A P T K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 E1290 A S G D E V D E F D A M V E G
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 D1359 E G I D F D S D D D G V E R E
Sea Urchin Strong. purpuratus XP_783546 1448 163750 L1291 E G S E L S G L M E D D K D S
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 A1308 L D L A Q R L A Q Y N F G S A
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 S1272 P S S T S S S S I F D I K K E
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 20 20 0 N.A. 40 53.3 N.A. 13.3 6.6 13.3 13.3 N.A. 6.6 N.A. 0 6.6
P-Site Similarity: 100 33.3 33.3 20 N.A. 60 53.3 N.A. 20 20 20 20 N.A. 20 N.A. 13.3 40
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 13.3 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 40 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 20 0 14 7 0 0 14 14 7 7 20 7 0 14 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 14 20 14 0 7 7 7 20 14 14 27 14 27 7 % D
% Glu: 20 20 0 20 7 7 0 7 0 14 7 0 7 20 40 % E
% Phe: 0 0 0 0 20 0 0 0 7 7 0 7 0 0 0 % F
% Gly: 14 14 7 0 7 0 14 0 7 14 7 0 7 0 7 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 7 0 0 0 0 0 7 0 0 7 0 0 0 % I
% Lys: 34 0 7 20 0 7 20 7 0 14 14 0 40 14 7 % K
% Leu: 7 7 27 0 7 0 7 7 0 0 14 0 7 14 7 % L
% Met: 0 0 0 0 0 0 0 0 7 7 0 14 0 0 0 % M
% Asn: 0 0 0 0 0 0 7 0 0 0 7 0 0 0 0 % N
% Pro: 7 14 0 20 20 14 27 0 7 7 0 0 7 0 0 % P
% Gln: 0 0 0 0 14 27 0 7 7 0 0 0 0 0 7 % Q
% Arg: 0 0 7 7 0 7 0 0 0 0 0 0 0 7 0 % R
% Ser: 0 14 20 0 7 14 20 40 14 0 20 7 0 14 7 % S
% Thr: 0 0 0 7 7 0 0 0 0 7 0 0 0 7 7 % T
% Val: 14 0 7 0 7 20 0 14 14 7 14 20 14 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 7 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _