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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 15.15
Human Site: S1476 Identified Species: 23.81
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.21
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 S1476 S T S D D S D S N F E K I V S
Chimpanzee Pan troglodytes XP_516332 1634 184611 K1523 K R A P K Q K K V V E A V N S
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 K1509 K R A P K Q K K V V E A V N S
Dog Lupus familis XP_537646 1532 174610 S1477 S T S D D S D S N F E K M I S
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 S1472 S S S D S S D S D F E R A I S
Rat Rattus norvegicus P41516 1526 173202 S1472 S S S D S S D S E F E K A I S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 W1506 G P E S S P G W L K G G E E E
Chicken Gallus gallus O42130 1553 174974 R1498 S D S D F G S R P S K S V A A
Frog Xenopus laevis NP_001082502 1579 178601 S1491 A K K P S S D S E D A A P A P
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 P1485 S D S D S A G P A V A K K Q A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 S1392 S D D D G N A S D D D S P K R
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 K1461 T D F F G A S K K N G K K A A
Sea Urchin Strong. purpuratus XP_783546 1448 163750 A1393 S G D A A L A A R L A A Q T D
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 N1410 V M S R L A D N K E E E S S E
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 E1374 R E D S N K D E D D A I P Q R
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 13.3 13.3 86.6 N.A. 60 66.6 N.A. 0 20 20 26.6 N.A. 20 N.A. 6.6 6.6
P-Site Similarity: 100 26.6 26.6 100 N.A. 86.6 80 N.A. 0 40 26.6 40 N.A. 40 N.A. 26.6 13.3
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 20 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 40 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 7 0 14 7 7 20 14 7 7 0 27 27 14 20 20 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 27 20 47 14 0 47 0 20 20 7 0 0 0 7 % D
% Glu: 0 7 7 0 0 0 0 7 14 7 47 7 7 7 14 % E
% Phe: 0 0 7 7 7 0 0 0 0 27 0 0 0 0 0 % F
% Gly: 7 7 0 0 14 7 14 0 0 0 14 7 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 7 7 20 0 % I
% Lys: 14 7 7 0 14 7 14 20 14 7 7 34 14 7 0 % K
% Leu: 0 0 0 0 7 7 0 0 7 7 0 0 0 0 0 % L
% Met: 0 7 0 0 0 0 0 0 0 0 0 0 7 0 0 % M
% Asn: 0 0 0 0 7 7 0 7 14 7 0 0 0 14 0 % N
% Pro: 0 7 0 20 0 7 0 7 7 0 0 0 20 0 7 % P
% Gln: 0 0 0 0 0 14 0 0 0 0 0 0 7 14 0 % Q
% Arg: 7 14 0 7 0 0 0 7 7 0 0 7 0 0 14 % R
% Ser: 54 14 47 14 34 34 14 40 0 7 0 14 7 7 40 % S
% Thr: 7 14 0 0 0 0 0 0 0 0 0 0 0 7 0 % T
% Val: 7 0 0 0 0 0 0 0 14 20 0 0 20 7 0 % V
% Trp: 0 0 0 0 0 0 0 7 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _