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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 38.79
Human Site: S527 Identified Species: 60.95
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 S527 K N Y E D E D S L K T L R Y G
Chimpanzee Pan troglodytes XP_516332 1634 184611 S556 K S Y D D A K S L K T L R Y G
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 S542 K S Y D D A E S L K T L R Y G
Dog Lupus familis XP_537646 1532 174610 S527 K N Y D D E D S L K T L R Y G
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 S526 K N Y E D E D S L K T L R Y G
Rat Rattus norvegicus P41516 1526 173202 S525 K N Y E D E D S L K T L R Y G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 S555 K N Y D D Q D S L K T L R Y G
Chicken Gallus gallus O42130 1553 174974 S528 K N Y E D R E S L K S L R Y G
Frog Xenopus laevis NP_001082502 1579 178601 S525 K N Y D D N E S L R T L R Y G
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 S526 K N Y S D P E S L K S L R Y G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 D507 K K Y L T E D D L K T L R Y G
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 D562 K K Y E T E D D F K T L R Y G
Sea Urchin Strong. purpuratus XP_783546 1448 163750 I535 E N A E I N N I I K I L G L Q
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 M520 I L G L K Q N M K Y E N V N S
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 K508 I M G L Q H R K K Y E D T K S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 73.3 73.3 93.3 N.A. 100 100 N.A. 86.6 80 73.3 73.3 N.A. 73.3 N.A. 73.3 26.6
P-Site Similarity: 100 86.6 93.3 100 N.A. 100 100 N.A. 100 93.3 93.3 86.6 N.A. 73.3 N.A. 73.3 46.6
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 0 0 N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 0 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 14 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 34 67 0 47 14 0 0 0 7 0 0 0 % D
% Glu: 7 0 0 40 0 40 27 0 0 0 14 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % F
% Gly: 0 0 14 0 0 0 0 0 0 0 0 0 7 0 80 % G
% His: 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 % H
% Ile: 14 0 0 0 7 0 0 7 7 0 7 0 0 0 0 % I
% Lys: 80 14 0 0 7 0 7 7 14 80 0 0 0 7 0 % K
% Leu: 0 7 0 20 0 0 0 0 74 0 0 87 0 7 0 % L
% Met: 0 7 0 0 0 0 0 7 0 0 0 0 0 0 0 % M
% Asn: 0 60 0 0 0 14 14 0 0 0 0 7 0 7 0 % N
% Pro: 0 0 0 0 0 7 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 7 14 0 0 0 0 0 0 0 0 7 % Q
% Arg: 0 0 0 0 0 7 7 0 0 7 0 0 80 0 0 % R
% Ser: 0 14 0 7 0 0 0 67 0 0 14 0 0 0 14 % S
% Thr: 0 0 0 0 14 0 0 0 0 0 67 0 7 0 0 % T
% Val: 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 80 0 0 0 0 0 0 14 0 0 0 80 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _