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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 44.85
Human Site: T965 Identified Species: 70.48
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 T965 R E Y H T D T T V K F V V K M
Chimpanzee Pan troglodytes XP_516332 1634 184611 T994 K E Y H T D T T V K F V V K M
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 T980 K E Y H T D T T V K F V V K M
Dog Lupus familis XP_537646 1532 174610 T965 R E Y H T D T T V K F V V K M
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 T964 R E Y H T D T T V K F V I K M
Rat Rattus norvegicus P41516 1526 173202 T963 R E Y H T D T T V K F V I K M
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 T993 R E Y H T D T T V K F I V K M
Chicken Gallus gallus O42130 1553 174974 T966 K E Y H T D T T V K F V V K M
Frog Xenopus laevis NP_001082502 1579 178601 T963 K E Y H T D T T V R F L V K M
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 D966 D Y K E Y H T D T T V R F V V
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 T945 R E Y H T D T T V R F V I S F
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 T1000 K E Y H T D T T V K F V V K L
Sea Urchin Strong. purpuratus XP_783546 1448 163750 E951 D N S V E I T E L P I R V W T
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 K954 V K A Y N D E K S V D F D L I
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 W929 G N D K I K P W I K D M E E Q
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 93.3 93.3 100 N.A. 93.3 93.3 N.A. 93.3 93.3 80 6.6 N.A. 73.3 N.A. 86.6 13.3
P-Site Similarity: 100 100 100 100 N.A. 100 100 N.A. 100 100 100 13.3 N.A. 86.6 N.A. 100 20
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 14 0 7 0 0 80 0 7 0 0 14 0 7 0 0 % D
% Glu: 0 74 0 7 7 0 7 7 0 0 0 0 7 7 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 74 7 7 0 7 % F
% Gly: 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 74 0 7 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 7 7 0 0 7 0 7 7 20 0 7 % I
% Lys: 34 7 7 7 0 7 0 7 0 67 0 0 0 67 0 % K
% Leu: 0 0 0 0 0 0 0 0 7 0 0 7 0 7 7 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 60 % M
% Asn: 0 14 0 0 7 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 7 0 0 7 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 % Q
% Arg: 40 0 0 0 0 0 0 0 0 14 0 14 0 0 0 % R
% Ser: 0 0 7 0 0 0 0 0 7 0 0 0 0 7 0 % S
% Thr: 0 0 0 0 74 0 87 74 7 7 0 0 0 0 7 % T
% Val: 7 0 0 7 0 0 0 0 74 7 7 60 60 7 7 % V
% Trp: 0 0 0 0 0 0 0 7 0 0 0 0 0 7 0 % W
% Tyr: 0 7 74 7 7 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _