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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: TOP2A All Species: 42.73
Human Site: Y686 Identified Species: 67.14
UniProt: P11388 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P11388 NP_001058.2 1531 174385 Y686 G L P E D Y L Y G Q T T T Y L
Chimpanzee Pan troglodytes XP_516332 1634 184611 Y715 G L P E Q F L Y G T A T K H L
Rhesus Macaque Macaca mulatta XP_001092092 1620 182490 Y701 G L P E Q F L Y G T A T K H L
Dog Lupus familis XP_537646 1532 174610 Y686 G L P E D Y L Y G Q T T T Y L
Cat Felis silvestris
Mouse Mus musculus Q01320 1528 172859 Y685 G L P E D Y L Y G Q S T S Y L
Rat Rattus norvegicus P41516 1526 173202 Y684 G L P E D Y L Y G Q T T M Y L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001519680 1746 195650 Y714 G L P E D Y L Y G K A T N Y L
Chicken Gallus gallus O42130 1553 174974 Y687 G L P E D Y L Y G K D T N Y L
Frog Xenopus laevis NP_001082502 1579 178601 Y684 G L P E E Y L Y G K S T K Y L
Zebra Danio Brachydanio rerio NP_001003834 1574 177254 Y685 N L P E E Y L Y G Q E T K S L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P15348 1447 164377 Y666 G L P E R Y L Y T K G T K S I
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q23670 1520 172316 Y721 G L A E E Y L Y N K D T R F V
Sea Urchin Strong. purpuratus XP_783546 1448 163750 S692 E D R K V W L S E W L E E R R
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P30182 1473 164089 L675 S Y V P G N H L D Q R Q P K V
Baker's Yeast Sacchar. cerevisiae P06786 1428 164196 L663 Q Y E P G T V L D P T L K E I
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 67.2 67.5 93.6 N.A. 89 88.7 N.A. 71.3 76.3 70.7 67.9 N.A. 51.2 N.A. 53 58.9
Protein Similarity: 100 78.6 78.4 96.4 N.A. 93.6 93.2 N.A. 79.1 85 81.8 78.8 N.A. 66.8 N.A. 70.4 73.4
P-Site Identity: 100 60 60 100 N.A. 86.6 93.3 N.A. 80 80 73.3 66.6 N.A. 53.3 N.A. 46.6 6.6
P-Site Similarity: 100 73.3 73.3 100 N.A. 100 93.3 N.A. 86.6 86.6 93.3 73.3 N.A. 66.6 N.A. 73.3 20
Percent
Protein Identity: N.A. N.A. N.A. 45 42.2 N.A.
Protein Similarity: N.A. N.A. N.A. 61.4 60.4 N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 6.6 N.A.
P-Site Similarity: N.A. N.A. N.A. 13.3 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 0 0 0 0 20 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 7 0 0 40 0 0 0 14 0 14 0 0 0 0 % D
% Glu: 7 0 7 80 20 0 0 0 7 0 7 7 7 7 0 % E
% Phe: 0 0 0 0 0 14 0 0 0 0 0 0 0 7 0 % F
% Gly: 74 0 0 0 14 0 0 0 67 0 7 0 0 0 0 % G
% His: 0 0 0 0 0 0 7 0 0 0 0 0 0 14 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14 % I
% Lys: 0 0 0 7 0 0 0 0 0 34 0 0 40 7 0 % K
% Leu: 0 80 0 0 0 0 87 14 0 0 7 7 0 0 67 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 7 0 0 % M
% Asn: 7 0 0 0 0 7 0 0 7 0 0 0 14 0 0 % N
% Pro: 0 0 74 14 0 0 0 0 0 7 0 0 7 0 0 % P
% Gln: 7 0 0 0 14 0 0 0 0 40 0 7 0 0 0 % Q
% Arg: 0 0 7 0 7 0 0 0 0 0 7 0 7 7 7 % R
% Ser: 7 0 0 0 0 0 0 7 0 0 14 0 7 14 0 % S
% Thr: 0 0 0 0 0 7 0 0 7 14 27 80 14 0 0 % T
% Val: 0 0 7 0 7 0 7 0 0 0 0 0 0 0 14 % V
% Trp: 0 0 0 0 0 7 0 0 0 7 0 0 0 0 0 % W
% Tyr: 0 14 0 0 0 67 0 80 0 0 0 0 0 47 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _