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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NEFH All Species: 15.45
Human Site: S347 Identified Species: 37.78
UniProt: P12036 Number Species: 9
    Phosphosite Substitution
    Charge Score: 0.11
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P12036 NP_066554.2 1026 112479 S347 D S L E R Q R S E L E D R H Q
Chimpanzee Pan troglodytes XP_515058 1018 111384 S347 D S L E R Q R S E L E D R H Q
Rhesus Macaque Macaca mulatta XP_001109476 915 99597 T274 N N N N K R Q T L S P S P D L
Dog Lupus familis XP_543237 862 97231 E278 K E I R S Q L E C H S D Q N M
Cat Felis silvestris
Mouse Mus musculus P19246 1090 116976 S345 E S L E R Q R S E L E D R H Q
Rat Rattus norvegicus P16884 1072 115359 S343 E S L E R Q R S E L E D R H Q
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001517087 696 75544 I120 K M A L D I E I A A Y R K L L
Chicken Gallus gallus P16053 858 95817 Q275 I R A Q L E C Q S D H N M H Q
Frog Xenopus laevis NP_001081681 913 102801 N331 E S L E R Q L N D I E E R H G
Zebra Danio Brachydanio rerio NP_001104684 849 95616 Q269 G H S T Q N L Q Q V E D W F M
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 97.6 81 38.2 N.A. 76.2 75.2 N.A. 38.4 40.4 35 39.1 N.A. N.A. N.A. N.A. N.A.
Protein Similarity: 100 97.7 83.6 53.1 N.A. 81.7 81.4 N.A. 46.2 54.1 52.5 54.2 N.A. N.A. N.A. N.A. N.A.
P-Site Identity: 100 100 0 13.3 N.A. 93.3 93.3 N.A. 0 13.3 53.3 13.3 N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: 100 100 40 33.3 N.A. 100 100 N.A. 6.6 33.3 86.6 33.3 N.A. N.A. N.A. N.A. N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 20 0 0 0 0 0 10 10 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 10 0 10 0 0 0 0 0 0 % C
% Asp: 20 0 0 0 10 0 0 0 10 10 0 60 0 10 0 % D
% Glu: 30 10 0 50 0 10 10 10 40 0 60 10 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 % F
% Gly: 10 0 0 0 0 0 0 0 0 0 0 0 0 0 10 % G
% His: 0 10 0 0 0 0 0 0 0 10 10 0 0 60 0 % H
% Ile: 10 0 10 0 0 10 0 10 0 10 0 0 0 0 0 % I
% Lys: 20 0 0 0 10 0 0 0 0 0 0 0 10 0 0 % K
% Leu: 0 0 50 10 10 0 30 0 10 40 0 0 0 10 20 % L
% Met: 0 10 0 0 0 0 0 0 0 0 0 0 10 0 20 % M
% Asn: 10 10 10 10 0 10 0 10 0 0 0 10 0 10 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 10 0 10 0 0 % P
% Gln: 0 0 0 10 10 60 10 20 10 0 0 0 10 0 50 % Q
% Arg: 0 10 0 10 50 10 40 0 0 0 0 10 50 0 0 % R
% Ser: 0 50 10 0 10 0 0 40 10 10 10 10 0 0 0 % S
% Thr: 0 0 0 10 0 0 0 10 0 0 0 0 0 0 0 % T
% Val: 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _