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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: BRAF All Species: 31.82
Human Site: T488 Identified Species: 50
UniProt: P15056 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.29
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P15056 NP_004324.2 766 84437 T488 A V K M L N V T A P T P Q Q L
Chimpanzee Pan troglodytes XP_001155024 767 84561 T488 A V K M L N V T A P T P Q Q L
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_532749 767 85780 T488 A V K M L N V T A P T P Q Q L
Cat Felis silvestris
Mouse Mus musculus P28028 804 88899 T525 A V K M L N V T A P T P Q Q L
Rat Rattus norvegicus P11345 648 72910 R391 P E Q L Q A F R N E V A V L R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001516512 799 90020 T520 A V K M L N V T A P T P Q Q L
Chicken Gallus gallus Q04982 806 89347 T528 A V K M L N V T A P T P Q Q L
Frog Xenopus laevis P09560 638 71941 F381 T P E Q L Q A F R N E V A V L
Zebra Danio Brachydanio rerio NP_991307 777 86403 T498 A V K M L N V T A P T P Q Q L
Tiger Blowfish Takifugu rubipres NP_001032957 778 86510 T499 A V K M L N V T A P T P Q Q L
Fruit Fly Dros. melanogaster P11346 782 88616 K503 A V K T L N V K T P S P A Q L
Honey Bee Apis mellifera XP_396892 715 80060 R451 K N E V A V L R K T R H V N I
Nematode Worm Caenorhab. elegans Q07292 813 90389 V512 A I K K L N V V D P T P S Q M
Sea Urchin Strong. purpuratus XP_781094 750 85072 K471 A V K R L N V K D P T P S Q L
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q05609 821 90288 A511 P P Q N M M R A S N Q I E A A
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.3 N.A. 92.9 N.A. 88.8 50.2 N.A. 85.7 89.8 49.3 83.5 82.9 42.8 50.6 30.7 49.3
Protein Similarity: 100 99.3 N.A. 93.4 N.A. 89.3 62.2 N.A. 86.7 90.9 61.3 87.5 87.1 57.9 65 46.6 62.6
P-Site Identity: 100 100 N.A. 100 N.A. 100 0 N.A. 100 100 13.3 100 100 66.6 0 60 73.3
P-Site Similarity: 100 100 N.A. 100 N.A. 100 13.3 N.A. 100 100 20 100 100 73.3 26.6 73.3 73.3
Percent
Protein Identity: N.A. N.A. N.A. 24 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 41.5 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 0 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 74 0 0 0 7 7 7 7 54 0 0 7 14 7 7 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 14 0 0 0 0 0 0 % D
% Glu: 0 7 14 0 0 0 0 0 0 7 7 0 7 0 0 % E
% Phe: 0 0 0 0 0 0 7 7 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % H
% Ile: 0 7 0 0 0 0 0 0 0 0 0 7 0 0 7 % I
% Lys: 7 0 74 7 0 0 0 14 7 0 0 0 0 0 0 % K
% Leu: 0 0 0 7 80 0 7 0 0 0 0 0 0 7 74 % L
% Met: 0 0 0 54 7 7 0 0 0 0 0 0 0 0 7 % M
% Asn: 0 7 0 7 0 74 0 0 7 14 0 0 0 7 0 % N
% Pro: 14 14 0 0 0 0 0 0 0 74 0 74 0 0 0 % P
% Gln: 0 0 14 7 7 7 0 0 0 0 7 0 54 74 0 % Q
% Arg: 0 0 0 7 0 0 7 14 7 0 7 0 0 0 7 % R
% Ser: 0 0 0 0 0 0 0 0 7 0 7 0 14 0 0 % S
% Thr: 7 0 0 7 0 0 0 54 7 7 67 0 0 0 0 % T
% Val: 0 67 0 7 0 7 74 7 0 0 7 7 14 7 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _