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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: H2AFX All Species: 17.88
Human Site: T7 Identified Species: 35.76
UniProt: P16104 Number Species: 11
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P16104 NP_002096.1 143 15145 T7 _ M S G R G K T G G K A R A K
Chimpanzee Pan troglodytes XP_520760 155 16635 Q7 _ M S G R G K Q G G K V R A K
Rhesus Macaque Macaca mulatta XP_001090149 155 16475 Q7 _ M S G R G K Q G G K V R A K
Dog Lupus familis XP_853256 242 25674 T106 S M S G R G K T G G K A R A K
Cat Felis silvestris
Mouse Mus musculus P27661 143 15124 T7 _ M S G R G K T G G K A R A K
Rat Rattus norvegicus P02262 130 14059 Q7 _ M S G R G K Q G G K A R A K
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001512371 142 15010 T7 _ M S G R G K T G G K A R A K
Chicken Gallus gallus P02263 129 13922 Q7 _ M S G R G K Q G G K A R A K
Frog Xenopus laevis Q6GM86 139 14837 A7 _ M S G R G K A V S K T R A K
Zebra Danio Brachydanio rerio Q7ZUY3 142 14983 T7 _ M S G R G K T G G K A R A K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P84051 124 13344 G7 _ M S G R G K G G K V K G K A
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus P16886 126 13432 G7 _ M S G R G K G A G K A R A K
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 79.3 79.3 58.6 N.A. 97.1 85.3 N.A. 93 85.3 84.6 87.4 N.A. 79 N.A. N.A. 81.8
Protein Similarity: 100 81.2 81.9 59 N.A. 99.3 86.7 N.A. 94.4 86.7 90.2 90.2 N.A. 83.2 N.A. N.A. 84.6
P-Site Identity: 100 85.7 85.7 93.3 N.A. 100 92.8 N.A. 100 92.8 71.4 100 N.A. 50 N.A. N.A. 85.7
P-Site Similarity: 100 85.7 85.7 93.3 N.A. 100 92.8 N.A. 100 92.8 71.4 100 N.A. 50 N.A. N.A. 85.7
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 9 9 0 0 67 0 92 9 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 100 0 100 0 17 84 84 0 0 9 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 0 0 0 0 0 0 100 0 0 9 92 9 0 9 92 % K
% Leu: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % L
% Met: 0 100 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 34 0 0 0 0 0 0 0 % Q
% Arg: 0 0 0 0 100 0 0 0 0 0 0 0 92 0 0 % R
% Ser: 9 0 100 0 0 0 0 0 0 9 0 0 0 0 0 % S
% Thr: 0 0 0 0 0 0 0 42 0 0 0 9 0 0 0 % T
% Val: 0 0 0 0 0 0 0 0 9 0 9 17 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 92 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _