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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MGMT All Species: 26.97
Human Site: S14 Identified Species: 65.93
UniProt: P16455 Number Species: 9
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P16455 NP_002403 207 21646 S14 M K R T T L D S P L G K L E L
Chimpanzee Pan troglodytes XP_001142169 207 21582 S14 M K H T T L D S P L G K L E L
Rhesus Macaque Macaca mulatta XP_001089785 207 21935 S14 M K R T V L D S P L G K L E L
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus P26187 211 22416 S14 M K Y S V L D S P L G K M E L
Rat Rattus norvegicus P24528 209 22225 S14 M K Y T V L D S P L G K I E L
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001521529 115 12511
Chicken Gallus gallus XP_421823 258 28080 S88 E K H A V L L S P V G K L E I
Frog Xenopus laevis
Zebra Danio Brachydanio rerio XP_684479 187 20183 S16 L C A V T L T S P V G E I L L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001191329 183 19555 T18 L A T V V L A T P I G H V T I
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P26188 188 21480 T12 L Y Y T F I E T E V T G A F L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 97.5 92.7 N.A. N.A. 68.7 68.4 N.A. 36.2 42.2 N.A. 42 N.A. N.A. N.A. N.A. 34.7
Protein Similarity: 100 98 94.1 N.A. N.A. 77.2 77 N.A. 40.5 52.3 N.A. 55.5 N.A. N.A. N.A. N.A. 50.2
P-Site Identity: 100 93.3 93.3 N.A. N.A. 73.3 80 N.A. 0 53.3 N.A. 40 N.A. N.A. N.A. N.A. 20
P-Site Similarity: 100 93.3 93.3 N.A. N.A. 86.6 86.6 N.A. 0 66.6 N.A. 66.6 N.A. N.A. N.A. N.A. 53.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. 25.6 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 37.2 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 46.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 10 10 10 0 0 10 0 0 0 0 0 10 0 0 % A
% Cys: 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 50 0 0 0 0 0 0 0 0 % D
% Glu: 10 0 0 0 0 0 10 0 10 0 0 10 0 60 0 % E
% Phe: 0 0 0 0 10 0 0 0 0 0 0 0 0 10 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 80 10 0 0 0 % G
% His: 0 0 20 0 0 0 0 0 0 0 0 10 0 0 0 % H
% Ile: 0 0 0 0 0 10 0 0 0 10 0 0 20 0 20 % I
% Lys: 0 60 0 0 0 0 0 0 0 0 0 60 0 0 0 % K
% Leu: 30 0 0 0 0 80 10 0 0 50 0 0 40 10 70 % L
% Met: 50 0 0 0 0 0 0 0 0 0 0 0 10 0 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 80 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 0 20 0 0 0 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 0 0 10 0 0 0 70 0 0 0 0 0 0 0 % S
% Thr: 0 0 10 50 30 0 10 20 0 0 10 0 0 10 0 % T
% Val: 0 0 0 20 50 0 0 0 0 30 0 0 10 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 10 30 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _