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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MGMT All Species: 11.52
Human Site: S199 Identified Species: 28.15
UniProt: P16455 Number Species: 9
    Phosphosite Substitution
    Charge Score: 0.33
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P16455 NP_002403 207 21646 S199 L K G A G A T S G S P P A G R
Chimpanzee Pan troglodytes XP_001142169 207 21582 S199 L K G A G A T S G S P P A G R
Rhesus Macaque Macaca mulatta XP_001089785 207 21935 S199 L K R A G A T S G S L P A G R
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus P26187 211 22416 T203 L K S S F E S T S S E P S G R
Rat Rattus norvegicus P24528 209 22225 E201 S W L K P S F E S S S P K P S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001521529 115 12511 P108 S R P T G S G P A P T S P D P
Chicken Gallus gallus XP_421823 258 28080 E249 K E W L L S H E K L Q K E K L
Frog Xenopus laevis
Zebra Danio Brachydanio rerio XP_684479 187 20183 L178 H E R K A V E L N P S Q S G K
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_001191329 183 19555 W174 K G I V L K E W L L K H E G A
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P26188 188 21480 E180 Q L L N N E K E N S L S L S R
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 97.5 92.7 N.A. N.A. 68.7 68.4 N.A. 36.2 42.2 N.A. 42 N.A. N.A. N.A. N.A. 34.7
Protein Similarity: 100 98 94.1 N.A. N.A. 77.2 77 N.A. 40.5 52.3 N.A. 55.5 N.A. N.A. N.A. N.A. 50.2
P-Site Identity: 100 100 86.6 N.A. N.A. 40 13.3 N.A. 6.6 0 N.A. 6.6 N.A. N.A. N.A. N.A. 6.6
P-Site Similarity: 100 100 86.6 N.A. N.A. 66.6 20 N.A. 20 13.3 N.A. 26.6 N.A. N.A. N.A. N.A. 6.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. 25.6 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 37.2 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 13.3 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 30 10 30 0 0 10 0 0 0 30 0 10 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 % D
% Glu: 0 20 0 0 0 20 20 30 0 0 10 0 20 0 0 % E
% Phe: 0 0 0 0 10 0 10 0 0 0 0 0 0 0 0 % F
% Gly: 0 10 20 0 40 0 10 0 30 0 0 0 0 60 0 % G
% His: 10 0 0 0 0 0 10 0 0 0 0 10 0 0 0 % H
% Ile: 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 % I
% Lys: 20 40 0 20 0 10 10 0 10 0 10 10 10 10 10 % K
% Leu: 40 10 20 10 20 0 0 10 10 20 20 0 10 0 10 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 10 10 0 0 0 20 0 0 0 0 0 0 % N
% Pro: 0 0 10 0 10 0 0 10 0 20 20 50 10 10 10 % P
% Gln: 10 0 0 0 0 0 0 0 0 0 10 10 0 0 0 % Q
% Arg: 0 10 20 0 0 0 0 0 0 0 0 0 0 0 50 % R
% Ser: 20 0 10 10 0 30 10 30 20 60 20 20 20 10 10 % S
% Thr: 0 0 0 10 0 0 30 10 0 0 10 0 0 0 0 % T
% Val: 0 0 0 10 0 10 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 10 10 0 0 0 0 10 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _