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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: PFKL All Species: 4.55
Human Site: Y633 Identified Species: 8.33
UniProt: P17858 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P17858 NP_002617.3 780 85018 Y633 R N E K C H D Y Y T T E F L Y
Chimpanzee Pan troglodytes XP_001150233 780 84936 Y633 R N E K C H D Y Y T T E F L Y
Rhesus Macaque Macaca mulatta XP_001096244 851 93251 N705 R N E K C N E N Y T T D F I F
Dog Lupus familis XP_544922 751 82459 H604 R N E K C H E H Y T T E F L Y
Cat Felis silvestris
Mouse Mus musculus P12382 780 85282 H633 R N E K C H E H Y T T E F L Y
Rat Rattus norvegicus P30835 780 85320 H633 R N E K C H E H Y T T E F L Y
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001514123 751 82475 H604 R N E K C H E H Y T T E F L Y
Chicken Gallus gallus XP_001232621 780 85127 H633 R N E K C H E H Y T T E F L Y
Frog Xenopus laevis NP_001091319 786 86623 N636 R N E N S N E N Y T T D F I Y
Zebra Danio Brachydanio rerio XP_698635 780 84600 H633 R N E K C H E H Y T T D F I H
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P52034 788 86630 E642 L R N E K A S E N Y S T D F I
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans Q27483 756 83282 K605 R Q V E T N E K D L E K L A I
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P16862 959 104599 G811 F E K A E G R G R F G K L I L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.6 63.8 90.1 N.A. 94 94.2 N.A. 86.6 86.7 68.4 77.5 N.A. 55.5 N.A. 40.1 N.A.
Protein Similarity: 100 99.6 77.3 93 N.A. 97.1 97.3 N.A. 91.9 93.8 83.7 89.6 N.A. 73 N.A. 58.2 N.A.
P-Site Identity: 100 100 60 86.6 N.A. 86.6 86.6 N.A. 86.6 86.6 53.3 66.6 N.A. 0 N.A. 6.6 N.A.
P-Site Similarity: 100 100 93.3 100 N.A. 100 100 N.A. 100 100 80 100 N.A. 13.3 N.A. 33.3 N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. 36.1 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 50.5 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 8 0 8 0 0 0 0 0 0 0 8 0 % A
% Cys: 0 0 0 0 70 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 0 16 0 8 0 0 24 8 0 0 % D
% Glu: 0 8 77 16 8 0 70 8 0 0 8 54 0 0 0 % E
% Phe: 8 0 0 0 0 0 0 0 0 8 0 0 77 8 8 % F
% Gly: 0 0 0 0 0 8 0 8 0 0 8 0 0 0 0 % G
% His: 0 0 0 0 0 62 0 47 0 0 0 0 0 0 8 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 31 16 % I
% Lys: 0 0 8 70 8 0 0 8 0 0 0 16 0 0 0 % K
% Leu: 8 0 0 0 0 0 0 0 0 8 0 0 16 54 8 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 77 8 8 0 24 0 16 8 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 85 8 0 0 0 0 8 0 8 0 0 0 0 0 0 % R
% Ser: 0 0 0 0 8 0 8 0 0 0 8 0 0 0 0 % S
% Thr: 0 0 0 0 8 0 0 0 0 77 77 8 0 0 0 % T
% Val: 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 16 77 8 0 0 0 0 62 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _