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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NCL All Species: 25.15
Human Site: S542 Identified Species: 55.33
UniProt: P19338 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0.1
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P19338 NP_005372.2 710 76614 S542 D A K E A L N S C N K R E I E
Chimpanzee Pan troglodytes XP_516145 1006 108719 Q814 F I K V P Q N Q N G K S K G Y
Rhesus Macaque Macaca mulatta XP_001116949 938 100743 S770 D A K E A L N S C N K R E I E
Dog Lupus familis XP_850477 699 75739 S531 D A K E A L N S C N K R E I E
Cat Felis silvestris
Mouse Mus musculus P09405 707 76705 S543 D A K E A L N S C N K M E I E
Rat Rattus norvegicus P13383 713 77129 S545 D A K E A L N S C N K M E I E
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509329 631 67414 S485 E A K E A L N S L N N V E I E
Chicken Gallus gallus P15771 694 75622 S517 D A K E A L N S C N N T E I E
Frog Xenopus laevis P20397 651 70177 E475 M D S C N N T E I E G R S I R
Zebra Danio Brachydanio rerio
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_797746 400 43419 V297 T C S N A R M V K S R K F A Y
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P27476 414 44517 G311 T E Q P K G F G Y V Q F S N M
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 70.3 74.7 91.6 N.A. 83.9 84.2 N.A. 65.6 63.2 55.6 N.A. N.A. N.A. N.A. N.A. 24
Protein Similarity: 100 70.4 75.4 94.9 N.A. 90.1 90.4 N.A. 73.9 76 68.4 N.A. N.A. N.A. N.A. N.A. 32.8
P-Site Identity: 100 20 100 100 N.A. 93.3 93.3 N.A. 73.3 86.6 13.3 N.A. N.A. N.A. N.A. N.A. 6.6
P-Site Similarity: 100 26.6 100 100 N.A. 93.3 93.3 N.A. 80 86.6 13.3 N.A. N.A. N.A. N.A. N.A. 26.6
Percent
Protein Identity: N.A. N.A. N.A. N.A. 23.1 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 35.4 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 13.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 64 0 0 73 0 0 0 0 0 0 0 0 10 0 % A
% Cys: 0 10 0 10 0 0 0 0 55 0 0 0 0 0 0 % C
% Asp: 55 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % D
% Glu: 10 10 0 64 0 0 0 10 0 10 0 0 64 0 64 % E
% Phe: 10 0 0 0 0 0 10 0 0 0 0 10 10 0 0 % F
% Gly: 0 0 0 0 0 10 0 10 0 10 10 0 0 10 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 10 0 0 0 0 0 0 10 0 0 0 0 73 0 % I
% Lys: 0 0 73 0 10 0 0 0 10 0 55 10 10 0 0 % K
% Leu: 0 0 0 0 0 64 0 0 10 0 0 0 0 0 0 % L
% Met: 10 0 0 0 0 0 10 0 0 0 0 19 0 0 10 % M
% Asn: 0 0 0 10 10 10 73 0 10 64 19 0 0 10 0 % N
% Pro: 0 0 0 10 10 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 10 0 0 10 0 10 0 0 10 0 0 0 0 % Q
% Arg: 0 0 0 0 0 10 0 0 0 0 10 37 0 0 10 % R
% Ser: 0 0 19 0 0 0 0 64 0 10 0 10 19 0 0 % S
% Thr: 19 0 0 0 0 0 10 0 0 0 0 10 0 0 0 % T
% Val: 0 0 0 10 0 0 0 10 0 10 0 10 0 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 10 0 0 0 0 0 19 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _