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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: RPS6KB1 All Species: 26.36
Human Site: S427 Identified Species: 41.43
UniProt: P23443 Number Species: 14
    Phosphosite Substitution
    Charge Score: -0.07
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P23443 NP_003152.1 525 59140 S427 E S V K E K F S F E P K I R S
Chimpanzee Pan troglodytes XP_523815 451 50966 N356 H P F F R H I N W E E L L A R
Rhesus Macaque Macaca mulatta XP_001109701 525 59511 S427 E S V K E K F S F E P K I R S
Dog Lupus familis XP_537702 525 59107 S427 E S V K E K F S F E P K I R S
Cat Felis silvestris
Mouse Mus musculus Q8BSK8 525 59199 S427 E S V K E K F S F E P K I R S
Rat Rattus norvegicus P67999 525 59113 S427 E S V K E K F S F E P K I R S
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509850 482 54026 S384 E S V K E K F S F E P K I R S
Chicken Gallus gallus P18652 752 84421 Q414 D S K V K P A Q P P L H S V V
Frog Xenopus laevis P10666 629 71268 S395 E E D A K K T S S P P V L S V
Zebra Danio Brachydanio rerio Q6PFQ0 740 83378 S417 H G S S A Q F S D V Y E L K E
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9V3I5 1207 137026 V1080 P P P G D N V V A R I P K R T
Honey Bee Apis mellifera XP_395876 456 51514 F360 S E D D V S Q F D K K F T T S
Nematode Worm Caenorhab. elegans Q21734 784 88102 K437 E E R K L I A K S V R S V P T
Sea Urchin Strong. purpuratus XP_781234 487 53968 L390 E S A N Q I F L G F T Y V A P
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q39030 471 53019 P375 K G L L Q K E P E R R L G S G
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 85.9 94.2 99.8 N.A. 99.2 99.6 N.A. 90.2 31.9 36.8 32.2 N.A. 20.8 54.6 31.2 59
Protein Similarity: 100 85.9 95.6 100 N.A. 99.4 99.6 N.A. 90.8 46.6 52.7 46.6 N.A. 31.3 68 43.6 70.8
P-Site Identity: 100 6.6 100 100 N.A. 100 100 N.A. 100 6.6 26.6 13.3 N.A. 6.6 6.6 13.3 20
P-Site Similarity: 100 26.6 100 100 N.A. 100 100 N.A. 100 20 40 40 N.A. 20 13.3 26.6 33.3
Percent
Protein Identity: N.A. N.A. N.A. 36.3 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 55 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 6.6 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 26.6 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 7 7 0 14 0 7 0 0 0 0 14 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 7 0 14 7 7 0 0 0 14 0 0 0 0 0 0 % D
% Glu: 60 20 0 0 40 0 7 0 7 47 7 7 0 0 7 % E
% Phe: 0 0 7 7 0 0 54 7 40 7 0 7 0 0 0 % F
% Gly: 0 14 0 7 0 0 0 0 7 0 0 0 7 0 7 % G
% His: 14 0 0 0 0 7 0 0 0 0 0 7 0 0 0 % H
% Ile: 0 0 0 0 0 14 7 0 0 0 7 0 40 0 0 % I
% Lys: 7 0 7 47 14 54 0 7 0 7 7 40 7 7 0 % K
% Leu: 0 0 7 7 7 0 0 7 0 0 7 14 20 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 7 0 7 0 7 0 0 0 0 0 0 0 % N
% Pro: 7 14 7 0 0 7 0 7 7 14 47 7 0 7 7 % P
% Gln: 0 0 0 0 14 7 7 7 0 0 0 0 0 0 0 % Q
% Arg: 0 0 7 0 7 0 0 0 0 14 14 0 0 47 7 % R
% Ser: 7 54 7 7 0 7 0 54 14 0 0 7 7 14 47 % S
% Thr: 0 0 0 0 0 0 7 0 0 0 7 0 7 7 14 % T
% Val: 0 0 40 7 7 0 7 7 0 14 0 7 14 7 14 % V
% Trp: 0 0 0 0 0 0 0 0 7 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 7 7 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _