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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: RPS6KB1 All Species: 24.55
Human Site: S53 Identified Species: 38.57
UniProt: P23443 Number Species: 14
    Phosphosite Substitution
    Charge Score: 0.14
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P23443 NP_003152.1 525 59140 S53 E G G Q L N E S M D H G G V G
Chimpanzee Pan troglodytes XP_523815 451 50966 E36 D I D L D Q P E D A G S E D E
Rhesus Macaque Macaca mulatta XP_001109701 525 59511 S53 E G G Q L N E S M D H G G V G
Dog Lupus familis XP_537702 525 59107 S53 E G G Q L N E S M D H G G V G
Cat Felis silvestris
Mouse Mus musculus Q8BSK8 525 59199 S53 E G G Q L N E S M D H G G V E
Rat Rattus norvegicus P67999 525 59113 S53 E G G Q L N E S M D H G G V G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509850 482 54026 V36 F E I S E T S V N R G P E K I
Chicken Gallus gallus P18652 752 84421 S51 V E K D L V D S T D K G E G V
Frog Xenopus laevis P10666 629 71268 E38 G R H T S K D E V V V K E I P
Zebra Danio Brachydanio rerio Q6PFQ0 740 83378 S37 D E P M E E E S Y T H C D E G
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q9V3I5 1207 137026 A218 S S T T P S Y A M P T S N S T
Honey Bee Apis mellifera XP_395876 456 51514 P39 L H P H T S T P V S L S S E G
Nematode Worm Caenorhab. elegans Q21734 784 88102 P66 S S I H I I Q P P P N N G F R
Sea Urchin Strong. purpuratus XP_781234 487 53968 Y39 E G A E G D S Y L A D G A Y E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q39030 471 53019 S42 D N L E L Q F S D V F G P M P
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 85.9 94.2 99.8 N.A. 99.2 99.6 N.A. 90.2 31.9 36.8 32.2 N.A. 20.8 54.6 31.2 59
Protein Similarity: 100 85.9 95.6 100 N.A. 99.4 99.6 N.A. 90.8 46.6 52.7 46.6 N.A. 31.3 68 43.6 70.8
P-Site Identity: 100 0 100 100 N.A. 93.3 100 N.A. 0 26.6 0 26.6 N.A. 6.6 6.6 6.6 20
P-Site Similarity: 100 6.6 100 100 N.A. 93.3 100 N.A. 0 33.3 20 33.3 N.A. 20 20 26.6 40
Percent
Protein Identity: N.A. N.A. N.A. 36.3 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 55 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 20 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 40 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 7 0 0 0 0 7 0 14 0 0 7 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 7 0 0 0 % C
% Asp: 20 0 7 7 7 7 14 0 14 40 7 0 7 7 0 % D
% Glu: 40 20 0 14 14 7 40 14 0 0 0 0 27 14 20 % E
% Phe: 7 0 0 0 0 0 7 0 0 0 7 0 0 7 0 % F
% Gly: 7 40 34 0 7 0 0 0 0 0 14 54 40 7 40 % G
% His: 0 7 7 14 0 0 0 0 0 0 40 0 0 0 0 % H
% Ile: 0 7 14 0 7 7 0 0 0 0 0 0 0 7 7 % I
% Lys: 0 0 7 0 0 7 0 0 0 0 7 7 0 7 0 % K
% Leu: 7 0 7 7 47 0 0 0 7 0 7 0 0 0 0 % L
% Met: 0 0 0 7 0 0 0 0 40 0 0 0 0 7 0 % M
% Asn: 0 7 0 0 0 34 0 0 7 0 7 7 7 0 0 % N
% Pro: 0 0 14 0 7 0 7 14 7 14 0 7 7 0 14 % P
% Gln: 0 0 0 34 0 14 7 0 0 0 0 0 0 0 0 % Q
% Arg: 0 7 0 0 0 0 0 0 0 7 0 0 0 0 7 % R
% Ser: 14 14 0 7 7 14 14 54 0 7 0 20 7 7 0 % S
% Thr: 0 0 7 14 7 7 7 0 7 7 7 0 0 0 7 % T
% Val: 7 0 0 0 0 7 0 7 14 14 7 0 0 34 7 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 7 7 7 0 0 0 0 7 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _