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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: POLR2A All Species: 30
Human Site: S229 Identified Species: 66
UniProt: P24928 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P24928 NP_000928.1 1970 217176 S229 H E I F K R I S D E E C F V L
Chimpanzee Pan troglodytes XP_511300 1913 211215 S229 H E I F K R I S D E E C F V L
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_858599 1896 209574 S231 H E I F K R I S D E E C F V L
Cat Felis silvestris
Mouse Mus musculus P08775 1970 217158 S229 H E I F K R I S D E E C F V L
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus Q5ZL98 1390 155685
Frog Xenopus laevis
Zebra Danio Brachydanio rerio XP_682682 1972 217631 A226 H E I F K R I A D E E D I I L
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P04052 1887 209150 T221 W E I L K H I T D E E C F I L
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P16356 1852 203961 T223 L E V F Q Q I T D E D I L V I
Sea Urchin Strong. purpuratus XP_001176260 1921 212544 S229 H E I F K R I S D E E S I A L
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana P18616 1840 204671 S227 L S V L K R I S D A D C Q L L
Baker's Yeast Sacchar. cerevisiae P04050 1733 191593 S215 L N I F K H I S V K D F T S L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 96.7 N.A. 95.9 N.A. 99.9 N.A. N.A. N.A. 26.2 N.A. 94.9 N.A. 71.1 N.A. 67.4 77.6
Protein Similarity: 100 96.8 N.A. 96 N.A. 99.9 N.A. N.A. N.A. 40.8 N.A. 97.8 N.A. 84.1 N.A. 81 87.2
P-Site Identity: 100 100 N.A. 100 N.A. 100 N.A. N.A. N.A. 0 N.A. 73.3 N.A. 66.6 N.A. 40 80
P-Site Similarity: 100 100 N.A. 100 N.A. 100 N.A. N.A. N.A. 0 N.A. 86.6 N.A. 80 N.A. 80 80
Percent
Protein Identity: N.A. N.A. N.A. 57.4 50.1 N.A.
Protein Similarity: N.A. N.A. N.A. 71.9 64.8 N.A.
P-Site Identity: N.A. N.A. N.A. 46.6 40 N.A.
P-Site Similarity: N.A. N.A. N.A. 66.6 53.3 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 0 0 0 0 10 0 10 0 0 0 10 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 55 0 0 0 % C
% Asp: 0 0 0 0 0 0 0 0 82 0 28 10 0 0 0 % D
% Glu: 0 73 0 0 0 0 0 0 0 73 64 0 0 0 0 % E
% Phe: 0 0 0 73 0 0 0 0 0 0 0 10 46 0 0 % F
% Gly: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % G
% His: 55 0 0 0 0 19 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 73 0 0 0 91 0 0 0 0 10 19 19 10 % I
% Lys: 0 0 0 0 82 0 0 0 0 10 0 0 0 0 0 % K
% Leu: 28 0 0 19 0 0 0 0 0 0 0 0 10 10 82 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 0 10 10 0 0 0 0 0 0 10 0 0 % Q
% Arg: 0 0 0 0 0 64 0 0 0 0 0 0 0 0 0 % R
% Ser: 0 10 0 0 0 0 0 64 0 0 0 10 0 10 0 % S
% Thr: 0 0 0 0 0 0 0 19 0 0 0 0 10 0 0 % T
% Val: 0 0 19 0 0 0 0 0 10 0 0 0 0 46 0 % V
% Trp: 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _