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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: U2AF2 All Species: 6.67
Human Site: S28 Identified Species: 13.33
UniProt: P26368 Number Species: 11
    Phosphosite Substitution
    Charge Score: -0.09
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P26368 NP_001012496.1 475 53501 S28 E N R H R K R S H S R S R S R
Chimpanzee Pan troglodytes XP_530073 713 78555 R145 P S R S G G G R S R S S L I G
Rhesus Macaque Macaca mulatta XP_001091568 467 51268 C24 A R G T R R E C A R D D P G V
Dog Lupus familis
Cat Felis silvestris
Mouse Mus musculus P26369 475 53499 S28 E N R H R K R S H S R S R S R
Rat Rattus norvegicus Q63285 419 46487
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus
Frog Xenopus laevis NP_001080595 456 51328 K26 D K E N R H R K R S R S R S K
Zebra Danio Brachydanio rerio NP_991252 475 53612 H31 E R D K E N R H R R R S P S R
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q24562 416 46636
Honey Bee Apis mellifera XP_623055 432 48801 D17 D R Q D R E K D R D R D R D R
Nematode Worm Caenorhab. elegans P90978 496 55412 R63 N D R K K R K R S R S R D R D
Sea Urchin Strong. purpuratus XP_001189425 444 50005 S20 E R D R R S R S K D N K R S R
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana Q8L716 589 66297 D134 H R R S R D R D R R R S R D R
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 53.4 87.5 N.A. N.A. 99.7 20 N.A. N.A. N.A. 92 86.3 N.A. 66.7 66.7 51.4 62.9
Protein Similarity: 100 57.2 89.2 N.A. N.A. 99.7 37.6 N.A. N.A. N.A. 94.7 92.2 N.A. 77 77.8 62.5 75.5
P-Site Identity: 100 13.3 6.6 N.A. N.A. 100 0 N.A. N.A. N.A. 46.6 40 N.A. 0 26.6 6.6 46.6
P-Site Similarity: 100 20 13.3 N.A. N.A. 100 0 N.A. N.A. N.A. 66.6 40 N.A. 0 53.3 33.3 46.6
Percent
Protein Identity: N.A. N.A. N.A. 37.6 N.A. N.A.
Protein Similarity: N.A. N.A. N.A. 50.7 N.A. N.A.
P-Site Identity: N.A. N.A. N.A. 46.6 N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. 46.6 N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 9 0 0 0 0 0 0 0 9 0 0 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 % C
% Asp: 17 9 17 9 0 9 0 17 0 17 9 17 9 17 9 % D
% Glu: 34 0 9 0 9 9 9 0 0 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 9 0 9 9 9 0 0 0 0 0 0 9 9 % G
% His: 9 0 0 17 0 9 0 9 17 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 % I
% Lys: 0 9 0 17 9 17 17 9 9 0 0 9 0 0 9 % K
% Leu: 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 9 17 0 9 0 9 0 0 0 0 9 0 0 0 0 % N
% Pro: 9 0 0 0 0 0 0 0 0 0 0 0 17 0 0 % P
% Gln: 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 % Q
% Arg: 0 42 42 9 59 17 50 17 34 42 50 9 50 9 50 % R
% Ser: 0 9 0 17 0 9 0 25 17 25 17 50 0 42 0 % S
% Thr: 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 % T
% Val: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _