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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: CHAT All Species: 0.3
Human Site: T109 Identified Species: 0.56
UniProt: P28329 Number Species: 12
    Phosphosite Substitution
    Charge Score: -0.25
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P28329 NP_066266.3 748 82536 T109 P A P G L T K T P I L E K V P
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta XP_001109211 626 70697 G26 S S L I K A S G R F K A H Q D
Dog Lupus familis XP_543902 686 76910 R86 Q T L A T Y L R C M Q H L V P
Cat Felis silvestris
Mouse Mus musculus Q03059 641 71834 Q41 Q T L A T Y L Q C M Q H L V P
Rat Rattus norvegicus P32738 640 71845 Q40 Q T L A T Y L Q C M Q H L V P
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001509108 643 72737 R43 K T L A M Y L R C M R H L V T
Chicken Gallus gallus Q90YJ9 640 72605 Q40 Q T L Q M Y L Q C M K H L V P
Frog Xenopus laevis Q7ZXE1 659 74538 F59 P R L P I P K F E D T I K R Y
Zebra Danio Brachydanio rerio B2ZGJ1 637 71821 M37 L D M Y L T C M G H L V P E D
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P07668 721 81310 P94 T L P K V P V P A L D E T M A
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P32756 627 71299 S27 L P K P P V P S L E A T L D R
Sea Urchin Strong. purpuratus XP_001185550 675 75787 L75 L E R Y L K S L S P L V S K E
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P32796 670 77223 K70 F Q G I T F A K Q Q D L P S L
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. 37.8 77.9 N.A. 74.7 74.7 N.A. 68.4 66 24 57 N.A. 37.5 N.A. 29.2 46.3
Protein Similarity: 100 N.A. 55.7 82.4 N.A. 79.1 79 N.A. 76.5 74 40.6 68.8 N.A. 56.1 N.A. 45.1 62.4
P-Site Identity: 100 N.A. 0 13.3 N.A. 13.3 13.3 N.A. 6.6 13.3 20 20 N.A. 13.3 N.A. 0 13.3
P-Site Similarity: 100 N.A. 6.6 20 N.A. 20 20 N.A. 20 26.6 26.6 20 N.A. 33.3 N.A. 6.6 13.3
Percent
Protein Identity: N.A. N.A. N.A. N.A. 27 N.A.
Protein Similarity: N.A. N.A. N.A. N.A. 44.2 N.A.
P-Site Identity: N.A. N.A. N.A. N.A. 0 N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. 0 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 8 0 31 0 8 8 0 8 0 8 8 0 0 8 % A
% Cys: 0 0 0 0 0 0 8 0 39 0 0 0 0 0 0 % C
% Asp: 0 8 0 0 0 0 0 0 0 8 16 0 0 8 16 % D
% Glu: 0 8 0 0 0 0 0 0 8 8 0 16 0 8 8 % E
% Phe: 8 0 0 0 0 8 0 8 0 8 0 0 0 0 0 % F
% Gly: 0 0 8 8 0 0 0 8 8 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 8 0 39 8 0 0 % H
% Ile: 0 0 0 16 8 0 0 0 0 8 0 8 0 0 0 % I
% Lys: 8 0 8 8 8 8 16 8 0 0 16 0 16 8 0 % K
% Leu: 24 8 54 0 24 0 39 8 8 8 24 8 47 0 8 % L
% Met: 0 0 8 0 16 0 0 8 0 39 0 0 0 8 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 16 8 16 16 8 16 8 8 8 8 0 0 16 0 39 % P
% Gln: 31 8 0 8 0 0 0 24 8 8 24 0 0 8 0 % Q
% Arg: 0 8 8 0 0 0 0 16 8 0 8 0 0 8 8 % R
% Ser: 8 8 0 0 0 0 16 8 8 0 0 0 8 8 0 % S
% Thr: 8 39 0 0 31 16 0 8 0 0 8 8 8 0 8 % T
% Val: 0 0 0 0 8 8 8 0 0 0 0 16 0 47 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 16 0 39 0 0 0 0 0 0 0 0 8 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _