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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NOS2 All Species: 15.15
Human Site: T121 Identified Species: 33.33
UniProt: P35228 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P35228 NP_000616.3 1153 131117 T121 S C L G S I M T P K S L T R G
Chimpanzee Pan troglodytes XP_001148238 1153 131137 T121 S C L G S I M T P K S L T R G
Rhesus Macaque Macaca mulatta XP_001106245 1153 130638 T121 S C L G S I M T P K S L T R G
Dog Lupus familis XP_534695 1431 160627 L339 I C M G S I M L P S Q Q I R R
Cat Felis silvestris
Mouse Mus musculus P29477 1144 130557 N115 S C L G S I M N P K S L T R G
Rat Rattus norvegicus Q06518 1147 130609 N118 L C M G S I M N S K S L T R G
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506887 1152 131150 N129 S C Q G A I M N P K T L V R G
Chicken Gallus gallus Q90703 1136 129631 T118 A C Q G A L M T P K G L V R S
Frog Xenopus laevis NP_001079155 1419 160392 T327 I C M G A V M T P P H Y V R K
Zebra Danio Brachydanio rerio NP_001098407 1079 121801 L119 A R I D R I A L E I T L T G T
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q27571 1349 151696 Q186 P S P K N Q Q Q P R K M S Q D
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.3 97.1 45.7 N.A. 80.6 80.4 N.A. 73.2 69.3 44.8 55.5 N.A. 40.4 N.A. N.A. N.A.
Protein Similarity: 100 99.4 98.2 59 N.A. 88.4 87.8 N.A. 84.6 82 59.2 70.5 N.A. 54.9 N.A. N.A. N.A.
P-Site Identity: 100 100 100 46.6 N.A. 93.3 73.3 N.A. 66.6 53.3 40 20 N.A. 6.6 N.A. N.A. N.A.
P-Site Similarity: 100 100 100 53.3 N.A. 93.3 80 N.A. 80 73.3 60 40 N.A. 40 N.A. N.A. N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 19 0 0 0 28 0 10 0 0 0 0 0 0 0 0 % A
% Cys: 0 82 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 10 0 0 0 0 0 0 0 0 0 0 10 % D
% Glu: 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 0 82 0 0 0 0 0 0 10 0 0 10 55 % G
% His: 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 % H
% Ile: 19 0 10 0 0 73 0 0 0 10 0 0 10 0 0 % I
% Lys: 0 0 0 10 0 0 0 0 0 64 10 0 0 0 10 % K
% Leu: 10 0 37 0 0 10 0 19 0 0 0 73 0 0 0 % L
% Met: 0 0 28 0 0 0 82 0 0 0 0 10 0 0 0 % M
% Asn: 0 0 0 0 10 0 0 28 0 0 0 0 0 0 0 % N
% Pro: 10 0 10 0 0 0 0 0 82 10 0 0 0 0 0 % P
% Gln: 0 0 19 0 0 10 10 10 0 0 10 10 0 10 0 % Q
% Arg: 0 10 0 0 10 0 0 0 0 10 0 0 0 82 10 % R
% Ser: 46 10 0 0 55 0 0 0 10 10 46 0 10 0 10 % S
% Thr: 0 0 0 0 0 0 0 46 0 0 19 0 55 0 10 % T
% Val: 0 0 0 0 0 10 0 0 0 0 0 0 28 0 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 10 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _