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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: RFC4 All Species: 12.12
Human Site: S25 Identified Species: 20.51
UniProt: P35249 Number Species: 13
    Phosphosite Substitution
    Charge Score: -0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P35249 NP_002907.1 363 39682 S25 K D R G V A A S A G S S G E N
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_535837 363 39624 A25 D R G I A A T A G S S G E N K
Cat Felis silvestris
Mouse Mus musculus Q99J62 364 39848 T25 K D R G T P A T A G S S G E T
Rat Rattus norvegicus NP_001099339 364 39938 S25 K E R G I S S S A G S S G E T
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001513440 259 28454
Chicken Gallus gallus NP_001006550 359 39305 S26 K E R S A A G S S G E G N K L
Frog Xenopus laevis NP_001082757 363 39992 T25 D K A S A S G T G S T G E S K
Zebra Danio Brachydanio rerio NP_999902 358 39865 G25 P S S S S S S G G E K K Q R A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster NP_573245 353 39536 R25 S Q G T P A A R R K P P A P W
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans P53016 334 37687 T25 T E K Y R P K T L D D I A Y Q
Sea Urchin Strong. purpuratus XP_787339 355 39574 Q25 S T G G S T K Q R Q V P W V E
Poplar Tree Populus trichocarpa XP_002307456 342 38023 V29 D V A H Q D E V V R V L T N T
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_564148 339 37489 V29 D V A H Q E E V V R V L T N T
Baker's Yeast Sacchar. cerevisiae P40348 353 39723 Q25 A E Q S L A Q Q P W V E K Y R
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. N.A. 95.3 N.A. 90.9 91.2 N.A. 61.7 83.1 80.4 74 N.A. 53.7 N.A. 39.1 61.4
Protein Similarity: 100 N.A. N.A. 98 N.A. 95.3 96.4 N.A. 67.4 90.9 90.9 87 N.A. 69.6 N.A. 58.9 76.5
P-Site Identity: 100 N.A. N.A. 13.3 N.A. 73.3 66.6 N.A. 0 33.3 0 0 N.A. 13.3 N.A. 0 6.6
P-Site Similarity: 100 N.A. N.A. 20 N.A. 80 93.3 N.A. 0 53.3 20 13.3 N.A. 13.3 N.A. 20 6.6
Percent
Protein Identity: 51.2 N.A. N.A. 52 50.1 N.A.
Protein Similarity: 68.3 N.A. N.A. 69.7 67.7 N.A.
P-Site Identity: 0 N.A. N.A. 0 6.6 N.A.
P-Site Similarity: 0 N.A. N.A. 0 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 8 0 22 0 22 36 22 8 22 0 0 0 15 0 8 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 29 15 0 0 0 8 0 0 0 8 8 0 0 0 0 % D
% Glu: 0 29 0 0 0 8 15 0 0 8 8 8 15 22 8 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 22 29 0 0 15 8 22 29 0 22 22 0 0 % G
% His: 0 0 0 15 0 0 0 0 0 0 0 0 0 0 0 % H
% Ile: 0 0 0 8 8 0 0 0 0 0 0 8 0 0 0 % I
% Lys: 29 8 8 0 0 0 15 0 0 8 8 8 8 8 15 % K
% Leu: 0 0 0 0 8 0 0 0 8 0 0 15 0 0 8 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 0 0 8 22 8 % N
% Pro: 8 0 0 0 8 15 0 0 8 0 8 15 0 8 0 % P
% Gln: 0 8 8 0 15 0 8 15 0 8 0 0 8 0 8 % Q
% Arg: 0 8 29 0 8 0 0 8 15 15 0 0 0 8 8 % R
% Ser: 15 8 8 29 15 22 15 22 8 15 29 22 0 8 0 % S
% Thr: 8 8 0 8 8 8 8 22 0 0 8 0 15 0 29 % T
% Val: 0 15 0 0 8 0 0 15 15 0 29 0 0 8 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 8 0 0 8 0 8 % W
% Tyr: 0 0 0 8 0 0 0 0 0 0 0 0 0 15 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _