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Search by protein name, UniProt number, IPI number, or 15 AA P-site sequence.
Updated: 2017 Aug. 1
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Warning
– Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite
Conservation Score
Human Protein:
RFC4
All Species:
10
Human Site:
S28
Identified Species:
16.92
UniProt:
P35249
Number Species:
13
Phosphosite Substitution
Charge Score:
0.08
Phosphosite
Sequences
Species
Species
Scientific Name
UniProt ID
NCBI Ref Seq ID
AA#
Mr(Da)
P-Site
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4
5
6
7
Human
Homo sapiens
P35249
NP_002907.1
363
39682
S28
G
V
A
A
S
A
G
S
S
G
E
N
K
K
A
Chimpanzee
Pan troglodytes
Rhesus Macaque
Macaca mulatta
Dog
Lupus familis
XP_535837
363
39624
S28
I
A
A
T
A
G
S
S
G
E
N
K
K
T
K
Cat
Felis silvestris
Mouse
Mus musculus
Q99J62
364
39848
S28
G
T
P
A
T
A
G
S
S
G
E
T
K
K
V
Rat
Rattus norvegicus
NP_001099339
364
39938
S28
G
I
S
S
S
A
G
S
S
G
E
T
K
K
V
Wallaby
Macropus eugenll
Platypus
Ornith. anatinus
XP_001513440
259
28454
Chicken
Gallus gallus
NP_001006550
359
39305
E29
S
A
A
G
S
S
G
E
G
N
K
L
K
P
V
Frog
Xenopus laevis
NP_001082757
363
39992
T28
S
A
S
G
T
G
S
T
G
E
S
K
K
Q
K
Zebra Danio
Brachydanio rerio
NP_999902
358
39865
K28
S
S
S
S
G
G
E
K
K
Q
R
A
V
P
W
Tiger Blowfish
Takifugu rubipres
Fruit Fly
Dros. melanogaster
NP_573245
353
39536
P28
T
P
A
A
R
R
K
P
P
A
P
W
V
E
K
Honey Bee
Apis mellifera
Nematode Worm
Caenorhab. elegans
P53016
334
37687
D28
Y
R
P
K
T
L
D
D
I
A
Y
Q
D
E
V
Sea Urchin
Strong. purpuratus
XP_787339
355
39574
V28
G
S
T
K
Q
R
Q
V
P
W
V
E
K
Y
R
Poplar Tree
Populus trichocarpa
XP_002307456
342
38023
V32
H
Q
D
E
V
V
R
V
L
T
N
T
L
E
T
Maize
Zea mays
Rice
Oryza sativa
Thale Cress
Arabidopsis thaliana
NP_564148
339
37489
V32
H
Q
E
E
V
V
R
V
L
T
N
T
L
Q
T
Baker's Yeast
Sacchar. cerevisiae
P40348
353
39723
V28
S
L
A
Q
Q
P
W
V
E
K
Y
R
P
K
N
Red Bread Mold
Neurospora crassa
Conservation
Percent
Protein Identity:
100
N.A.
N.A.
95.3
N.A.
90.9
91.2
N.A.
61.7
83.1
80.4
74
N.A.
53.7
N.A.
39.1
61.4
Protein Similarity:
100
N.A.
N.A.
98
N.A.
95.3
96.4
N.A.
67.4
90.9
90.9
87
N.A.
69.6
N.A.
58.9
76.5
P-Site Identity:
100
N.A.
N.A.
20
N.A.
66.6
66.6
N.A.
0
26.6
6.6
0
N.A.
13.3
N.A.
0
13.3
P-Site Similarity:
100
N.A.
N.A.
26.6
N.A.
73.3
86.6
N.A.
0
40
33.3
13.3
N.A.
20
N.A.
13.3
13.3
Percent
Protein Identity:
51.2
N.A.
N.A.
52
50.1
N.A.
Protein Similarity:
68.3
N.A.
N.A.
69.7
67.7
N.A.
P-Site Identity:
0
N.A.
N.A.
0
13.3
N.A.
P-Site Similarity:
6.6
N.A.
N.A.
6.6
20
N.A.
Phosphosite
Consensus
Position
-7
-6
-5
-4
-3
-4
-5
0
+1
+2
+3
+4
+5
+6
+7
% Ala:
0
22
36
22
8
22
0
0
0
15
0
8
0
0
8
% A
% Cys:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% C
% Asp:
0
0
8
0
0
0
8
8
0
0
0
0
8
0
0
% D
% Glu:
0
0
8
15
0
0
8
8
8
15
22
8
0
22
0
% E
% Phe:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% F
% Gly:
29
0
0
15
8
22
29
0
22
22
0
0
0
0
0
% G
% His:
15
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% H
% Ile:
8
8
0
0
0
0
0
0
8
0
0
0
0
0
0
% I
% Lys:
0
0
0
15
0
0
8
8
8
8
8
15
50
29
22
% K
% Leu:
0
8
0
0
0
8
0
0
15
0
0
8
15
0
0
% L
% Met:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% M
% Asn:
0
0
0
0
0
0
0
0
0
8
22
8
0
0
8
% N
% Pro:
0
8
15
0
0
8
0
8
15
0
8
0
8
15
0
% P
% Gln:
0
15
0
8
15
0
8
0
0
8
0
8
0
15
0
% Q
% Arg:
0
8
0
0
8
15
15
0
0
0
8
8
0
0
8
% R
% Ser:
29
15
22
15
22
8
15
29
22
0
8
0
0
0
0
% S
% Thr:
8
8
8
8
22
0
0
8
0
15
0
29
0
8
15
% T
% Val:
0
8
0
0
15
15
0
29
0
0
8
0
15
0
29
% V
% Trp:
0
0
0
0
0
0
8
0
0
8
0
8
0
0
8
% W
% Tyr:
8
0
0
0
0
0
0
0
0
0
15
0
0
8
0
% Y
% Spaces:
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
% _