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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: POLR2I All Species: 33.03
Human Site: T66 Identified Species: 55.9
UniProt: P36954 Number Species: 13
    Phosphosite Substitution
    Charge Score: 0
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P36954 NP_006224.1 125 14523 T66 T H E V D E L T Q I I A D V S
Chimpanzee Pan troglodytes
Rhesus Macaque Macaca mulatta
Dog Lupus familis XP_867729 162 18139 T66 T H E V D E L T Q I I A D V S
Cat Felis silvestris
Mouse Mus musculus Q9CQZ7 108 12312 L53 L K E V D D V L G G A A A W E
Rat Rattus norvegicus
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_423952 125 14361 T66 T H E V D E L T Q I I A D V S
Frog Xenopus laevis NP_001089293 125 14410 T66 T H E I D E L T Q I I A D V A
Zebra Danio Brachydanio rerio NP_001006013 126 14576 T67 T H E V D E L T Q I I A D V A
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster P36958 129 15079 T70 M H E I D E L T H I V P D V I
Honey Bee Apis mellifera XP_396002 130 15292 T71 M H E I D E L T H I V A D V I
Nematode Worm Caenorhab. elegans NP_505062 167 19316 T108 V H E I D E L T Q I V G D I I
Sea Urchin Strong. purpuratus XP_788078 130 15070 T71 T H E V D E L T Q I V A E V V
Poplar Tree Populus trichocarpa XP_002312337 114 13195 L59 V A E R T Q V L Q D V A A D P
Maize Zea mays NP_001150634 114 12939 V58 S A G E F T Q V L Q D V A G D
Rice Oryza sativa
Thale Cress Arabidopsis thaliana NP_188323 114 13258 L59 V S E R T Q I L T D V A S D P
Baker's Yeast Sacchar. cerevisiae P27999 122 14270 P66 V Q D I G S D P T L P R S D R
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 N.A. N.A. 69.1 N.A. 25.6 N.A. N.A. N.A. 94.4 93.5 92.8 N.A. 73.6 72.3 47.9 79.2
Protein Similarity: 100 N.A. N.A. 70.9 N.A. 40.7 N.A. N.A. N.A. 97.5 98.4 95.2 N.A. 82.9 83.8 61 87.6
P-Site Identity: 100 N.A. N.A. 100 N.A. 26.6 N.A. N.A. N.A. 100 86.6 93.3 N.A. 60 66.6 60 80
P-Site Similarity: 100 N.A. N.A. 100 N.A. 40 N.A. N.A. N.A. 100 100 100 N.A. 73.3 80 80 93.3
Percent
Protein Identity: 51.2 53.6 N.A. 58.4 41.6 N.A.
Protein Similarity: 72.8 71.2 N.A. 74.4 60 N.A.
P-Site Identity: 20 0 N.A. 13.3 0 N.A.
P-Site Similarity: 40 6.6 N.A. 33.3 20 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 15 0 0 0 0 0 0 0 0 8 72 22 0 15 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 8 0 72 8 8 0 0 15 8 0 58 22 8 % D
% Glu: 0 0 86 8 0 65 0 0 0 0 0 0 8 0 8 % E
% Phe: 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 0 0 8 0 8 0 0 0 8 8 0 8 0 8 0 % G
% His: 0 65 0 0 0 0 0 0 15 0 0 0 0 0 0 % H
% Ile: 0 0 0 36 0 0 8 0 0 65 36 0 0 8 22 % I
% Lys: 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 % K
% Leu: 8 0 0 0 0 0 65 22 8 8 0 0 0 0 0 % L
% Met: 15 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % M
% Asn: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % N
% Pro: 0 0 0 0 0 0 0 8 0 0 8 8 0 0 15 % P
% Gln: 0 8 0 0 0 15 8 0 58 8 0 0 0 0 0 % Q
% Arg: 0 0 0 15 0 0 0 0 0 0 0 8 0 0 8 % R
% Ser: 8 8 0 0 0 8 0 0 0 0 0 0 15 0 22 % S
% Thr: 43 0 0 0 15 8 0 65 15 0 0 0 0 0 0 % T
% Val: 29 0 0 43 0 0 15 8 0 0 43 8 0 58 8 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 % W
% Tyr: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _