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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: MLH1 All Species: 13.94
Human Site: S374 Identified Species: 25.56
UniProt: P40692 Number Species: 12
    Phosphosite Substitution
    Charge Score: 0.08
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P40692 NP_000240.1 756 84601 S374 T S S S T S G S S D K V Y A H
Chimpanzee Pan troglodytes XP_001170274 756 84655 S374 T S S S T S G S S D K V Y A H
Rhesus Macaque Macaca mulatta XP_001087274 583 64794 V224 R S I F G N A V S R E L I E I
Dog Lupus familis XP_534219 757 84567 G374 T P S S T G S G D K V Y A H Q
Cat Felis silvestris
Mouse Mus musculus Q9JK91 760 84662 S374 A S S S T S G S G D K V Y A Y
Rat Rattus norvegicus P97679 757 84522 S374 A S S S T S G S G D K V H A Y
Wallaby Macropus eugenll
Platypus Ornith. anatinus
Chicken Gallus gallus XP_418828 757 84832 S374 S T A A K G T S D K V Y A H Q
Frog Xenopus laevis NP_001090545 750 84121 E371 S T S Q K S S E K V Y A H Q M
Zebra Danio Brachydanio rerio NP_956953 724 80793 P365 A S S S S A D P Q E R V Y A H
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster
Honey Bee Apis mellifera XP_001120100 716 81818 I353 T R L P K A D I T K E V L K E
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus XP_793318 745 83320 V371 R S S S T D Q V Y A H H M V R
Poplar Tree Populus trichocarpa XP_002325965 747 83627 N365 A V E S K L R N S N E A R T F
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae P38920 769 87044 Q374 R N R K S L R Q A Q V V E N S
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 99.5 76 93 N.A. 88.1 87 N.A. N.A. 80 75.1 69.7 N.A. N.A. 50.6 N.A. 60.3
Protein Similarity: 100 99.8 76.5 96.1 N.A. 93.9 93.3 N.A. N.A. 88.1 85.9 81 N.A. N.A. 70.7 N.A. 75.7
P-Site Identity: 100 100 13.3 26.6 N.A. 80 73.3 N.A. N.A. 6.6 13.3 46.6 N.A. N.A. 13.3 N.A. 26.6
P-Site Similarity: 100 100 33.3 26.6 N.A. 86.6 86.6 N.A. N.A. 33.3 33.3 73.3 N.A. N.A. 33.3 N.A. 26.6
Percent
Protein Identity: 34.7 N.A. N.A. N.A. 36 N.A.
Protein Similarity: 56.6 N.A. N.A. N.A. 58.5 N.A.
P-Site Identity: 13.3 N.A. N.A. N.A. 6.6 N.A.
P-Site Similarity: 33.3 N.A. N.A. N.A. 26.6 N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 31 0 8 8 0 16 8 0 8 8 0 16 16 39 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 0 0 8 16 0 16 31 0 0 0 0 0 % D
% Glu: 0 0 8 0 0 0 0 8 0 8 24 0 8 8 8 % E
% Phe: 0 0 0 8 0 0 0 0 0 0 0 0 0 0 8 % F
% Gly: 0 0 0 0 8 16 31 8 16 0 0 0 0 0 0 % G
% His: 0 0 0 0 0 0 0 0 0 0 8 8 16 16 24 % H
% Ile: 0 0 8 0 0 0 0 8 0 0 0 0 8 0 8 % I
% Lys: 0 0 0 8 31 0 0 0 8 24 31 0 0 8 0 % K
% Leu: 0 0 8 0 0 16 0 0 0 0 0 8 8 0 0 % L
% Met: 0 0 0 0 0 0 0 0 0 0 0 0 8 0 8 % M
% Asn: 0 8 0 0 0 8 0 8 0 8 0 0 0 8 0 % N
% Pro: 0 8 0 8 0 0 0 8 0 0 0 0 0 0 0 % P
% Gln: 0 0 0 8 0 0 8 8 8 8 0 0 0 8 16 % Q
% Arg: 24 8 8 0 0 0 16 0 0 8 8 0 8 0 8 % R
% Ser: 16 54 62 62 16 39 16 39 31 0 0 0 0 0 8 % S
% Thr: 31 16 0 0 47 0 8 0 8 0 0 0 0 8 0 % T
% Val: 0 8 0 0 0 0 0 16 0 8 24 54 0 8 0 % V
% Trp: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 0 0 0 0 0 0 8 0 8 16 31 0 16 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _